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[mucosal leishmaniasis by contiguity with a skin lesion: another case report from tunisia].three clinical forms of cutaneous leishmaniasis can be found in tunisia, i.e. the sporadic form due to leishmania infantum in the north, the zoonotic epidemic form due to leishmania major in the center and southwest, and the chronic cutaneous form due to leishmania tropica in the south. unlike cutaneous forms, mucosal involvement is uncommon. the purpose of this report is to describe another case of mucosal leishmaniasis due to contiguity with a skin lesion in a 54-years-old woman. the patient r ...200819639835
identification of tunisian leishmania spp. by pcr amplification of cysteine proteinase b (cpb) genes and phylogenetic analysis.discrimination of the old world leishmania parasites is important for diagnosis and epidemiological studies of leishmaniasis. we have developed pcr assays that allow the discrimination between leishmania major, leishmania tropica and leishmania infantum tunisian species. the identification was performed by a simple pcr targeting cysteine protease b (cpb) gene copies. these pcr can be a routine molecular biology tools for discrimination of leishmania spp. from different geographical origins and d ...201323228525
activity of olive leaf extracts against the promastigote stage of leishmania species and their correlation with the antioxidant activity.leishmaniasis is one of the neglected tropical diseases in terms of drug discovery and development. furthermore, the chemotherapy used to treat this disease has been proved to be highly toxic and to present resistance issues. as consequent, the need for novel leishmanicidal molecules has notably increased in the recent years. in the present work an attempt was made to evaluate the antioxidant and leishmanicidal activities besides presence of compounds in leaf extracts of 5 different tunisian oli ...201424662269
natural infection of north african gundi (ctenodactylus gundi) by leishmania tropica in the focus of cutaneous leishmaniasis, southeast tunisia.north african gundis (ctenodactylus gundi) were trapped in the leishmania (l.) tropica focus of cutaneous leishmaniasis, situated in southeast tunisia and evaluated for leishmania infection by real-time kinetoplast dna polymerase chain reaction (pcr). species identification was performed by internal transcribed spacer one (its1)-pcr-restriction fragment length polymorphism (rflp) and high-resolution melting (hrm) analysis of the 7sl rna gene. real-time pcr on blood was positive in 6 of 13 (46.2% ...022665601
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