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abundance, parity, and japanese encephalitis virus infection of mosquitoes (diptera:culicidae) in sepang district, malaysia.a 2-yr study of japanese encephalitis (je) virus in sepang district, selangor, malaysia, was carried out to identify the mosquito vectors and to determine their seasonal abundance, parity, and infection rates. in total, 81,889 mosquitoes belonging to 9 genera and > 50 species were identified from cdc trap collections augmented with dry ice during 1992 and 1993. culex tritaeniorhynchus giles and culex gelidus giles were the most abundant species, and both increased in numbers with increases in ra ...19979151487
repellent properties of celery, apium graveolens l., compared with commercial repellents, against mosquitoes under laboratory and field conditions.in our search for new bioactive products against mosquito vectors, we reported the slightly larvicidal and adulticidal potency, but remarkable repellency of apium graveolens both in laboratory and field conditions. repellency of the ethanolic preparation of hexane-extracted a. graveolens was, therefore, investigated and compared with those of 15 commercial mosquito repellents including the most widely used, deet. hexane-extracted a. graveolens showed a significant degree of repellency in a dose- ...200516262746
laboratory colonization of aedes lineatopennis.aedes lineatopennis, a species member of the subgenus neomelaniconion, could be colonized for more than 10 successive generations from 30 egg batches [totally 2,075 (34-98) eggs] of wild-caught females. the oviposited eggs needed to be incubated in a moisture chamber for at least 7 days to complete embryonation and, following immersion in 0.25-2% hay-fermented water, 61-66% of them hatched after hatching stimulation. larvae were easily reared in 0.25-1% hay-fermented water, with suspended powder ...200516438203
nonspecific patterns of vector, host and avian malaria parasite associations in a central african rainforest.malaria parasites use vertebrate hosts for asexual multiplication and culicidae mosquitoes for sexual and asexual development, yet the literature on avian malaria remains biased towards examining the asexual stages of the life cycle in birds. to fully understand parasite evolution and mechanism of malaria transmission, knowledge of all three components of the vector-host-parasite system is essential. little is known about avian parasite-vector associations in african rainforests where numerous s ...201021134011
emergence of genotype i of japanese encephalitis virus as the dominant genotype in asia.japanese encephalitis virus (jev), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis worldwide. previous phylogenetic studies based on the envelope protein indicated that there are four genotypes, and surveillance data suggest that genotype i is gradually replacing genotype iii as the dominant strain. here we report an evolutionary analysis based on 98 full-length genome sequences of jev, including 67 new samples isolated from humans, pigs, mosquitoes, midges. ...201121697481
mosquito communities and disease risk influenced by land use change and seasonality in the australian tropics.anthropogenic land use changes have contributed considerably to the rise of emerging and re-emerging mosquito-borne diseases. these diseases appear to be increasing as a result of the novel juxtapositions of habitats and species that can result in new interchanges of vectors, diseases and hosts. we studied whether the mosquito community structure varied between habitats and seasons and whether known disease vectors displayed habitat preferences in tropical australia.201627388293
comparison of genotypes i and iii in japanese encephalitis virus reveals distinct differences in their genetic and host diversity.japanese encephalitis (je) is an arthropod-borne disease associated with the majority of viral encephalitis cases in the asia-pacific region. the causative agent, japanese encephalitis virus (jev), has been phylogenetically divided into five genotypes. recent surveillance data indicate that genotype i (gi) is gradually replacing genotype iii (giii) as the dominant genotype. to investigate the mechanism behind the genotype shift and the potential consequences in terms of vaccine efficacy, human c ...201425056890
insights into the evolutionary history of japanese encephalitis virus (jev) based on whole-genome sequences comprising the five genotypes.japanese encephalitis virus (jev) is the etiological agent of japanese encephalitis (je), one of the most serious viral encephalitis worldwide. five genotypes have been classified based on phylogenetic analysis of the viral envelope gene or the complete genome. previous studies based on four genotypes have reported that in evolutionary terms, genotype 1 jev is the most recent lineage. however, until now, no systematic phylogenetic analysis was reported based on whole genomic sequence of all five ...201525884184
entomo-virological study of a suspected japanese encephalitis outbreak in muzaffarpur district, bihar, india. 023760390
molecular detection and genotyping of japanese encephalitis virus in mosquitoes during a 2010 outbreak in the republic of korea.japanese encephalitis virus (jev), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis. to reduce the impact of japanese encephalitis among children in the republic of korea (rok), the government established a mandatory vaccination program in 1967. through the efforts of this program only 0-7 (mean 2.1) cases of japanese encephalitis were reported annually in the rok during the period of 1984-2009. however, in 2010 there was an outbreak of 26 confirmed cases of j ...201323390520
assessment of angelica sinensis (oliv.) diels as a repellent for personal protection against mosquitoes under laboratory and field conditions in northern thailand.angelica sinensis (oliv.) hexane extract (ahe) has been reported as a proven and impressive repellent against laboratory-reared female aedes aegypti mosquitoes. with the aim of promoting products of plant origin as a viable alternative to conventional synthetic substances, this study was designed to transform ahe-based repellents for exploitable commercial production by enhancing their efficacy and assessing their physical and biological stability as well as repellency against mosquitoes under l ...201627357395
mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
analyzing mosquito (diptera: culicidae) diversity in pakistan by dna barcoding.although they are important disease vectors mosquito biodiversity in pakistan is poorly known. recent epidemics of dengue fever have revealed the need for more detailed understanding of the diversity and distributions of mosquito species in this region. dna barcoding improves the accuracy of mosquito inventories because morphological differences between many species are subtle, leading to misidentifications.201424827460
making mosquito taxonomy useful: a stable classification of tribe aedini that balances utility with current knowledge of evolutionary relationships.the tribe aedini (family culicidae) contains approximately one-quarter of the known species of mosquitoes, including vectors of deadly or debilitating disease agents. this tribe contains the genus aedes, which is one of the three most familiar genera of mosquitoes. during the past decade, aedini has been the focus of a series of extensive morphology-based phylogenetic studies published by reinert, harbach, and kitching (rh&k). those authors created 74 new, elevated or resurrected genera from wha ...201526226613
population genetics of two key mosquito vectors of rift valley fever virus reveals new insights into the changing disease outbreak patterns in kenya.rift valley fever (rvf) outbreaks in kenya have increased in frequency and range to include northeastern kenya where viruses are increasingly being isolated from known (aedes mcintoshi) and newly-associated (ae. ochraceus) vectors. the factors contributing to these changing outbreak patterns are unclear and the population genetic structure of key vectors and/or specific virus-vector associations, in particular, are under-studied. by conducting mitochondrial and nuclear dna analyses on >220 kenya ...201425474018
the transmission potential of rift valley fever virus among livestock in the netherlands: a modelling study.rift valley fever virus (rvfv) is a zoonotic vector-borne infection and causes a potentially severe disease. many mammals are susceptible to infection including important livestock species. although currently confined to africa and the near-east, this disease causes concern in countries in temperate climates where both hosts and potential vectors are present, such as the netherlands. currently, an assessment of the probability of an outbreak occurring in this country is missing. to evaluate the ...201323876054
geographic distribution of wolbachial infections in mosquitoes from thailand.members of the genus wolbachia are inherited intracellular bacterial endosymbionts that infect a diverse range of arthropods. here i report the results of a survey of these endosymbionts in different mosquito species from six geographic regions of northern, northeastern, western, central, eastern and southern thailand. using gene amplification assays with wsp and groe gene primers, wolbachiae were detected in 999 mosquitoes representing 28 species of 1622 specimens collected representing 74 spec ...201323660513
scanning electron microscopy of aedes lineatopennis (diptera: culicidae) eggs.the eggs of aedes lineatopennis (ludlow) are described by means of a scanning electron microscopy. the ae. lineatopennis egg has a rather boat-shaped, fragmented micropylar collar, and membrane-like wall enclosing many tubercles of exochorionic sculpture.200111580052
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