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comparative studies on the stenogamous and eurygamous behavior of eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand.establishment of laboratory colony is essential for mosquito-borne-disease research. mating behavior of stenogamous anopheles peditaeniatus and seven eurygamous species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (=an. lesteri), anopheles pursati and anopheles sinensis), were investigated and compared in this study. the self-mating success of adult mosquitoes in different size cages at two density resting surface (drs) values, 3.6 and 7. ...201627023618
unprecedented genomic diversity of rna viruses in arthropods reveals the ancestry of negative-sense rna viruses.although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. through rna sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense rna viruses, a number of which are also present as endogenous genomic copies. with this greatly enriched diversity we revealed that arthropods contain ...201525633976
genetic compatibility between anopheles lesteri from korea and anopheles paraliae from thailand.to assess differentiation and relationships between anopheles lesteri and anopheles paraliae we established three and five iso-female lines of an. lesteri from korea and an. paraliae from thailand, respectively. these isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing dna sequences of ribosomal dna second internal transcribed spacer (its2) and mitochondrial dna cytochrome c oxidase subunit i (coi) and subunit ii (coii). resul ...023778670
mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids - a review.constant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. one of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. the voltage-gated sodium channel (nav) is the target of pyrethroids and ddt insecticides, used to control insects of medical, agricultur ...201425292318
scanning electron microscopy of antennal sensilla of the eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand.antennal sensilla were first investigated in the eight medically and veterinary important anopheles mosquito species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (= anopheles lesteri), anopheles peditaeniatus, anopheles pursati, and anopheles sinensis) of the hyrcanus group in thailand, using scanning electron microscopy (sem). four types of sensilla, including sensilla chaetica (large and small), sensilla trichodea (sharp- and blunt-tipp ...201727752768
scanning electron microscopy of anopheles hyrcanus group (diptera: culicidae) eggs in thailand and an ultrastructural key for species identification.the eggs of anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae, anopheles peditaeniatus, anopheles pursati, and anopheles sinensis are described with the aid of scanning electron micrographs. comparisons of the egg structure among the eight species showed that the eggs differed with respect to the following characteristics: the deck-complete (an. argyropus, an. nigerrimus, an. paraliae, an. peditaeniatus, and an. sinensis); variable (complete, s ...201424337511
dna barcoding for the identification of eight species members of the thai hyrcanus group and investigation of their stenogamous behavior.eight species members of the thai hyrcanus group were identified based on the intact morphology and molecular analysis (coi barcoding, 658 bp) of f1-progenies. five iso-female lines of each species were pooled in order to establish stock colonies. a stenogamous colony of each species was investigated by making 200 and 300 newly emerged adult females and males co-habit in a 30 cm cubic cage for one week. after ovipositon, the spermathecae of females were examined for sperms. the results revealed ...201324161242
metaphase karyotypes of anopheles paraliae (diptera:culicidae) in thailand and evidence to support five cytological races.sixteen isoline colonies of anopheles paraliae were established from wild-caught females collected from cow-baited traps at 4 locations in thailand. they showed 3 types of x (x1, x2, x3) and 5 types of y (y1, y2, y3, y4, y5) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were designated as forms a (x3, y1), b (x1, x2, x3, y2), c (x3, y3), d (x1, x2, x3, y4) and e (x3, y5). form a was found in songkhla province, form b was o ...201323959489
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