| further studies on human enteric coronaviruses. | comparisons were made between human enteric coronaviruses and the enteric coronaviruses of pigs and calves by negative staining. examination of human intestinal organ culture fluids at various time intervals after inoculation with the human enteric coronavirus showed increasing numbers of particles in the fluids. thin sections of the columnar epithelial cells of these explants showed a number of features consistent with the replication of known human and animal coronaviruses. virus particles fou ... | 1977 | 196571 |
| enteric coronavirus-like particles in sheep. | | 1978 | 687304 |
| a study of naturally occurring feline coronavirus infections in kittens. | feline coronavirus is a common infection in cats, as indicated by the high prevalence of antibodies against the virus, especially in multicat households. approximately 5 to 12 per cent of seropositive cats develop classical feline infectious peritonitis. a survey of kittens born into households of seropositive cats demonstrated the existence of healthy coronavirus carriers. seronegative animals did not appear to excrete virus. no specific antibody titre could be linked to carrier status and some ... | 1992 | 1313617 |
| genomic organization and expression of the 3' end of the canine and feline enteric coronaviruses. | the genomic organization at the 3' end of canine coronavirus (ccv) and feline enteric coronavirus (fecv) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (fipv) and transmissible gastroenteritis virus (tgev) of swine. comparison of the latter two has previously revealed an extra open reading frame (orf) at the 3' end of the fipv genome, lacking in tgev, which is currently designated orf 6b. both ccv and fecv possess 6b-related orfs at the 3' ends of ... | 1992 | 1329312 |
| differential in vitro inhibition of feline enteric coronavirus and feline infectious peritonitis virus by actinomycin d. | the growth of feline enteric coronavirus strain 79-1683 in whole feline embryo cells was inhibited by the presence of 1 microgram/ml of actinomycin d in the culture fluid. no virus-specific mrnas could be detected in such cultures and yields of infectious virus were depressed by > 99%. by contrast, the antigenically related feline infectious peritonitis virus strain 79-1146 was unaffected by the presence of actinomycin d, indicating a fundamental difference between the two feline coronavirus str ... | 1992 | 1335030 |
| agents associated with neonatal diarrhoea in ethiopian dairy calves. | faeces samples collected from diarrhoeic dairy calves in the first 8 weeks of life were examined for the presence of 5 enteropathogens. the majority of the 108 diarrhoea cases occurred in the first 5 weeks of life and a commercial elisa kit detected bovine enteric coronavirus (bec) in 38.9%, serogroup a rotavirus (rv) in 16.7% and k99 (f5) fimbrial adhesin-positive escherichia coli (k99 etec) in 11.1 per cent. concurrent infections of these enteropathogens were detected in 14.8% of samples (30.8 ... | 1992 | 1339036 |
| bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of s1, s2 and he. | forty-four monoclonal antibodies (mabs) to the g110 isolate of bovine enteric coronavirus were used for the characterization of the peplomer proteins s and he. fourteen of these mabs reacted with he and the remaining 30 with the products of the s gene, s1 (19 mabs), s2 (six mabs) and gp200 (five mabs). s1 and he were found to carry major neutralization determinants, and s1 appeared to elicit the production of the mabs displaying the highest neutralizing activity. the topography of the epitopes w ... | 1992 | 1378480 |
| monoclonal antibody analysis of neutralization and antibody-dependent enhancement of feline infectious peritonitis virus. | fifty-four monoclonal antibodies (mabs) to feline infectious peritonitis virus (fipv) were characterized according to protein specificity, immunoglobulin subclass, virus neutralization, reactivity with different coronaviruses, and ability to induce antibody-dependent enhancement (ade) of fipv infection in vitro. the mabs were found to be specific for one of three structural proteins of fipv. a total of 47 mabs were specific for the 205-kda spike protein (s), 3 mabs were specific for the 45-kda n ... | 1992 | 1383568 |
| radioactive and enzymatic cloned cdna probes for bovine enteric coronavirus detection by molecular hybridization. | genomic rna of f15 strain bovine enteric coronavirus (becv) was cloned in e. coli. three clones (174, 160, pg78), selected in the cdna library, including a large portion of the nucleocapsid (n), matrix (m) and peplomeric (s) protein genes, were used as probes for a slot blot hybridization assay. two probe labelling techniques were compared, radiolabelling with 32p and enzymatic labelling through covalent linkage to peroxidase and chemiluminescence detection. the radioactive probe 174 detected as ... | 1992 | 1642553 |
| perspectives on the epizootiology of feline enteric coronavirus and the pathogenesis of feline infectious peritonitis. | this review presents some current thoughts regarding the epizootiology of the feline coronaviruses; feline infectious peritonitis virus (fipv) and feline coronavirus (fecv) with primary emphasis on the pathogenesis of these viruses in nature. although the mechanism(s) whereby fipv causes disease are still incompletely understood, there have been significant contributions to the literature over the past decade which provide a framework upon which plausible explanations can be postulated. two conc ... | 1991 | 1654668 |
| serologic evidence of coronavirus infection in new york and new england dairy cattle with winter dysentery. | acute and convalescent sera were collected from 8 dairy herds with classic clinical features of winter dysentery. an enzyme-linked immunosorbent assay was used to measure coronavirus antibody titers, employing calf diarrhea coronavirus as antigen. twenty-two of the 35 animals tested (63%) showed a greater than or equal to 4-fold seroconversion. adult cattle in all 8 herds seroconverted. these findings complement previously reported immunoperoxidase and electron microscopic evidence, suggesting a ... | 1991 | 1662077 |
| four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal half of spike glycoprotein s. | four major antigenic sites have been delineated on the spike protein (s) of the porcine enteric coronavirus transmissible gastroenteritis virus (tgev) in previous topological studies using monoclonal antibodies (mabs). correlation of these sites with the physical structure of the protein was achieved by use of different approaches. recombinant pex plasmids directing the synthesis of various fused s polypeptides were constructed. a hybrid protein containing nine s-specific residues (363 to 371) w ... | 1990 | 1693663 |
| characterization of monoclonal antibodies against feline infectious peritonitis virus type ii and antigenic relationship between feline, porcine, and canine coronaviruses. | seven monoclonal antibodies (mabs) with neutralizing activity against feline infectious peritonitis virus (fipv) strain 79-1149 (type ii) were prepared. when the polypeptide specificity recognized by these monoclonal antibodies (mabs) was investigated by western immunoblotting, all of the mabs reacted with peplomer glycoprotein (s) of the virus. by competitive binding assay these mabs were found to recognize at least 3 different epitopes. the reactivity of these mabs with 6 viruses classified as ... | 1991 | 1706593 |
| sequence and analysis of becv f15 matrix protein. | clones from the bovine enteric coronavirus (f15) cdna library were cloned in pbr322 and sequenced by the method of sanger and coulson. this led to the identification of a sequence of 1,300 bases which contained a single open reading frame of 690 bases yielding a protein having properties of the matrix protein (m). it was comprised of 230 amino acids with a molecular weight of 26,376 da. it was hydrophobic and had a net charge of +8 at neutral ph. analysis of its secondary structure could not est ... | 1990 | 1706882 |
| enteric coronavirus tgev: mapping of four major antigenic determinants in the amino half of peplomer protein e 2. | | 1990 | 1715657 |
| topological and functional analysis of epitopes on the s(e2) and he(e3) glycoproteins of bovine enteric coronavirus. | monoclonal antibodies (mabs) were selected which reacted with bovine enteric coronavirus s and he. mabs to s were used to identify 2 cleavage products of s, s/gp105 and s/gp90. monoclonals to s/gp105 and he neutralised the virus; only mabs to the latter inhibited haemagglutination and acetyl-esterase activity. topological distribution of epitopes was studied on these 3 glycoproteins by means of competition binding experiments. two independent epitopes were characterised on he, 4 on s/gp105, and ... | 1990 | 1715659 |
| characterization and location of the structural polypeptides of turkey enteric coronavirus using monoclonal antibodies and enzymatic treatments. | | 1990 | 1715664 |
| differentiation of transmissible gastroenteritis virus from porcine respiratory coronavirus and other antigenically related coronaviruses by using cdna probes specific for the 5' region of the s glycoprotein gene. | two cdna clones prepared from the virulent miller strain of transmissible gastroenteritis virus (tgev) were identified, and their nucleotide sequences were determined. the clones were nonoverlapping and located in the 5' region of the s glycoprotein gene. their nucleotide and predicted amino acid sequences were compared with published sequences of the attenuated purdue strain of tgev and feline infectious peritonitis virus (fipv). tgev clone pe21 contained 381 bp of the s glycoprotein gene and h ... | 1991 | 1847152 |
| sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus. | the 3' end of the turkey coronavirus (tcv) genome and the gene encoding the nucleocapsid protein (n) were cloned and sequenced. the gene encoding the membrane protein (m) was obtained by cloning a polymerase chain reaction (pcr)-amplified fragment obtained using bovine coronavirus (bcv)-specific primers. furthermore, five tcv dna fragments, obtained by pcr on rna from clinical specimens and corresponding to either the n terminus of the m protein or the complete m protein were also cloned and seq ... | 1991 | 1856695 |
| serum antibodies to human enteric coronavirus-like particles in australia, south africa, indonesia, niue, and papua new guinea. | relatively high levels of antibody to human enteric coronavirus-like particles were detected in the sera from rural aborigines in australia. levels were generally much lower in the sera from urban aborigines, and extremely low to not detectable in the sera from europeans. antibody to coronavirus-like particles was also detected in the sera from rural blacks from south africa, in the sera from indonesia and niue, and also possibly in the sera from rural villagers from papua new guinea, but in the ... | 1990 | 1980397 |
| nucleotide sequence of the glycoprotein s gene of bovine enteric coronavirus and comparison with the s proteins of two mouse hepatitis virus strains. | the gene encoding the spike glycoprotein (s) of bovine enteric coronavirus (becv) was cloned and its complete sequence of 4092 nucleotides was determined. this sequence contained a single long open reading frame with a coding capacity of 1363 amino acids (mr 150,747). the predicted protein had 19 n-glycosylation sites. a signal sequence comprising 17 amino acids was observed starting from the first methionine residue. a potential peptidase cleavage site was located between amino acids 763 and 76 ... | 1990 | 2155300 |
| antigenic and genomic relationships among turkey and bovine enteric coronaviruses. | antigenic and genomic relationships among tissue culture-adapted turkey enteric coronavirus (tcv) isolates, three strains of avian infectious bronchitis virus (ibv), and mammalian coronaviruses were investigated. immunoblotting and immunoprecipitation experiments using polyclonal antisera showed that the four major structural proteins of tcv cross-reacted with the four homologous proteins of bovine enteric coronavirus (bcv), the n and m proteins of mouse hepatitis virus serotype 3, and the n pro ... | 1990 | 2159566 |
| experimental infection of young rabbits with a rabbit enteric coronavirus. | the clinical signs and lesions caused by the rabbit enteric coronavirus (recv) were studied in young rabbits orally inoculated with a suspension containing recv particles. the inoculated animals were observed daily for evidence of diarrhea. fecal samples and specimens from the small intestine and from the gut associated lymphoid tissue (galt) were collected from 2 h to 29 days postinoculation (pi) and processed for immune electron microscopy (iem) and light microscopy. coronavirus particles were ... | 1990 | 2174299 |
| nucleotide sequence of the bovine enteric coronavirus becv f15 mrna 5 and mrna 6 unique regions. | | 1990 | 2320429 |
| detection of bovine enteric coronavirus in clinical specimens by hybridization with cdna probes. | molecular hybridization, previously optimized for purified bovine coronavirus (bcv), was adapted for detection of virus in clinical specimens. for this purpose, the accuracy of the existing tests had to be improved and suitable means for removal of extraneous molecules had to be established. six radioactive probes were used to obtain adequate detection signals. these probes, containing the complete n and e1 gene sequences and other sequences, hybridized to about 1/4 of the total length of the vi ... | 1990 | 2366761 |
| antigenic and polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. | twenty-nine hybridoma cells lines, producing monoclonal antibodies (mabs) to the minnesota strain of turkey enteric coronavirus (tcv), have been established by fusion of sp2/0 myeloma cells with spleen cells from balb/c mice immunized with purified preparations of the egg-adapted or tissue culture-adapted virus. the hybridomas produced mainly igg2a or igg1 antibodies. western immunoblotting experiments with purified virus, and immunoprecipitation tests with [35s]methionine-labelled infected cell ... | 1989 | 2472464 |
| detection of turkey enteric coronavirus by enzyme-linked immunosorbent assay and differentiation from other coronaviruses. | a double-antibody elisa for the detection of coronaviruses in intestinal contents from turkey poults with diarrhea was developed. antibodies were raised in rabbits and guinea pigs against a minnesota isolate of turkey enteric coronavirus (tcv) propagated in embryonating turkey eggs and were purified by density-gradient centrifugation. the specificity of antisera was confirmed by hemagglutination-inhibition and immunoelectron microscopy. absorption of anti-tcv hyperimmune sera with egg extracts o ... | 1989 | 2541641 |
| comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis. | a coronavirus which was isolated from a cheetah (acinonyx jubatus) that succumbed to feline infectious peritonitis was characterized in vitro. the virus was determined to be highly cell-associated with crandell feline kidney (crfk) cells and was routinely maintained as a persistent infection (crfk 83-4497). the cheetah coronavirus was compared with other members of the feline coronavirus group including the feline enteric coronavirus (fecv) 79-1683 and the feline infectious peritonitis viruses ( ... | 1989 | 2546331 |
| ultrastructure and protein a-gold immunolabelling of hrt-18 cells infected with turkey enteric coronavirus. | the minnesota strain of turkey enteric coronavirus (tcv) was propagated in hrt-18 cells, a cell line derived from human rectum adenocarcinoma. a productive non-cytopathic infection was established, without a previous adaptation, in these cells as shown by the specific hemagglutinating activity in cell culture supernatants. a post-embedding immunochemical technique, using specific antiserum directed against the original egg-adapted virus and colloidal-gold-labelled protein a as the electron-dense ... | 1989 | 2548321 |
| characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: establishment of an efficient elisa for antigen detection in feces. | monoclonal antibodies to bovine enteric coronavirus (bec) were produced. additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. the antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (elisa) and immunoblotting. neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40,000. only one out of 908 hybridoma colonies tested secreted antibodies wit ... | 1989 | 2549679 |
| isolation and trypsin-enhanced propagation of turkey enteric (bluecomb) coronaviruses in a continuous human rectal adenocarcinoma cell line. | turkey enteric coronavirus (tcv) from intestinal contents of diarrheal poults was isolated and serially propagated in hrt-18 cells, an established cell line derived from a human rectal adenocarcinoma. in these cells, tcv induced cytopathic changes, including polykaryocytosis, which depended on trypsin in the medium and incubation at 41 c. viral antigens could be demonstrated in the cytoplasm by immunofluorescence, and extracellular virus was detected by an elisa and negative electron microscopy. ... | 1989 | 2551201 |
| identification and location of the structural glycoproteins of a tissue culture-adapted turkey enteric coronavirus. | the minnesota strain of turkey enteric coronavirus (tcv) was grown on a human rectal tumor (hrt-18) cell line in the presence of radiolabeled amino acids and glucosamine to analyse virion structural proteins. in addition to the 52,000 unglycosylated nucleocapsid protein, three major glycoprotein species were found to be associated with the viral envelope. a predominant glycosylated protein with a molecular weight of 22-24,000 represented the transmembrane matrix protein. larger glycoproteins wit ... | 1989 | 2673155 |
| synthesis and processing of the bovine enteric coronavirus haemagglutinin protein. | the haemagglutinin molecule on the bovine enteric coronavirus has been identified as a glycoprotein of 140k composed of disulphide-linked subunits of 65k. in this study, we have shown the subunits to be identical by demonstrating an unambiguous amino-terminal amino acid sequence. the unglycosylated subunit was found to have an mr of 42.5k and to undergo rapid disulphide linkage and glycosylation. glycosylation was found to be of the asparagine-linked type and some of the oligosaccharides underwe ... | 1989 | 2732694 |
| intracellular synthesis and processing of the structural glycoproteins of turkey enteric coronavirus. | pulse labeling of cells with [35s]methionine or [3h]glucosamine at different times after infection, followed by sds-page and western immunoblotting analysis using rabbit anti-tcv hyperimmune serum, was used to resolve and identify tcv-induced intracellular proteins. the viral structural proteins (gp 200, gp 140/gp 66, gp 100/gp 120, p 52, and gp 24/p 20) were detected in radiolabeled cell extracts by 9 to 12 hours post-infection, as well as two possible non-structural proteins with apparent mol. ... | 1989 | 2774975 |
| diagnosis of porcine and bovine enteric coronavirus infections using cloned cdna probes. | molecular clones representing the first 2,000 bases from the 3' end of the porcine transmissible gastroenteritis coronavirus genome and the first 2,160 bases from the 3' end of the bovine enteric coronavirus genome were used in dot blot hybridization assays to detect viral rna from cell culture and from fecal specimens. in each case, the cloned dna represents approximately 10% of the genome. the cloned sequence for each virus encompasses the 3' noncoding region, the nucleocapsid protein gene, an ... | 1987 | 2821059 |
| enteric coronavirus tgev: partial sequence of the genomic rna, its organization and expression. | the sequence of the 3'-most 8300 nucleotides of the genome rna of the purdue-115 strain of the transmissible gastroenteritis virus tgev, a porcine coronavirus, was determined from cdna clones. the available sequence corresponds to the part of the genome (total length greater than 20 kb) expressed through subgenomic mrnas. the 5 subgenomic and the genomic rna species detected in tgev-infected cells form a 3'-coterminal 'nested' structure, a unique feature of coronaviridae. the transcription initi ... | 1987 | 2825819 |
| nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. | cdna clones mapping within the first 2601 bases of the 3' end of the tgev genome were sequenced completely or in part by the method of maxam and gilbert and open reading frames were examined. one reading frame yielding a protein having properties of the matrix (m) protein was identified. it is positioned at the immediate 5' side of the nucleocapsid (n) gene but is separated by an intergenic region of 12 bases. the deduced m protein is comprised of 262 amino acids, has a molecular weight of 29,54 ... | 1987 | 2829520 |
| viral agents associated with outbreaks of diarrhea in turkey flocks in quebec. | the relative importance of various enteric viruses associated with diarrhea of turkey poults was investigated by an evaluation of specimens received since 1982. specimens originated from one to eight week old turkey poults, with mild to severe diarrhea, from 114 flocks in 42 commercial operations located in southern quebec. the acute phase of enteritis occurred usually in poults between two and four weeks of age. clarified intestinal contents were examined by direct electron microscopy and enzym ... | 1988 | 2832044 |
| identification of the structural proteins of turkey enteric coronavirus. | coronaviruses in the intestinal contents of turkey poults from quebec flocks with outbreaks of enteritis were detected by electron microscopy. five isolates were serially propagated in embryonic turkey eggs by inoculation of clarified intestinal contents into the amniotic cavity. viral particles contained in the intestinal contents of infected embryos were purified by differential and isopycnic centrifugation in sucrose gradients. intact virions present in fractions of densities 1.18 to 1.20 g/m ... | 1988 | 2835946 |
| biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah. | an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ... | 1988 | 2849387 |
| comparison of serologic assays for measurement of antibody response to coronavirus in cats. | serologic virus neutralization tests, indirect immunofluorescence tests, and elisa, using tissue culture-adapted feline infectious peritonitis virus (fipv) or feline enteric coronavirus (fecv) were compared for their ability to distinguish specific virus exposure in cats. sera of specific-pathogen-free cats inoculated with virulent or modified fipv or fecv were used to compare the sensitivity and specificity of the homologous assays to a heterologous assay that measures antibody reactivity with ... | 1988 | 2851952 |
| human enteric coronaviruses: antigenic relatedness to human coronavirus oc43 and possible etiologic role in viral gastroenteritis. | in a group of infants with acute nonbacterial gastroenteritis, examination of paired sera for antibody to human coronavirus (hcv) oc43 and neonatal calf diarrhea coronavirus showed a peculiar pattern of serological response, restricted only to hcv oc43 surface antigens, in a significantly higher proportion than among age-matched controls. in another group of infants and young children with acute nonbacterial gastroenteritis, fecal excretion of coronavirus-like particles was detected by electron ... | 1985 | 2985710 |
| an enteric coronavirus of the rabbit: detection by immunoelectron microscopy and identification of structural polypeptides. | the immunoelectron microscopy (iem) technique has been used for the detection of a rabbit enteric coronavirus (recv). immune serum was prepared in guinea pigs; the viral antigen used for the immunization procedure was obtained from the caecum of a sick rabbit, concentrated by centrifugation and purified on percoll gradient. in order to identify the viral particles used in the immunization procedure, the protein pattern of the particles was determined by electrophoresis and compared with the patt ... | 1985 | 2986579 |
| studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. | an immunofluorescence test on smears of nasal epithelial cells was used to detect coronavirus infection in the respiratory tract of calves. thirteen gnotobiotic calves were infected with coronavirus isolates derived from faeces or respiratory material: virus was detected in faeces and nasal swabs from all animals. in 115 calves from a field survey, there was a significant association between coronavirus excretion from both respiratory and enteric routes in calves with diarrhoea. in a further 12 ... | 1985 | 2990390 |
| chronic shedding of bovine enteric coronavirus antigen-antibody complexes by clinically normal cows. | using an elisa for the detection of virus-specific immune complexes, ten cows were found to be shedding bovine enteric coronavirus. the shedding patterns from five of these animals were followed for a period of 12 weeks, and all were found to be chronically shedding virus. despite the presence of both faecal and serum antibody the infection was not cleared; therefore, the role of cell-mediated immunity (cmi) was investigated by immunosuppressing the chronically shedding cows with dexamethasone. ... | 1985 | 2991425 |
| isolation and propagation of a human enteric coronavirus. | coronavirus-like particles were found by electron microscopy in stools from infants with necrotizing enterocolitis. stool samples from these infants as well as control specimens were passaged in cultures of human fetal intestinal organs. two samples yielded virus-like particles and these have now been passaged 14 times (hec 14). gradient-purified hec 14 strains had typical coronavirus morphology on electron microscopy and contained five major proteins with molecular sizes ranging from 190 to 23 ... | 1985 | 2992091 |
| intraepithelial leukocytes contain a unique subpopulation of nk-like cytotoxic cells active in the defense of gut epithelium to enteric murine coronavirus. | initially the intraepithelial leukocytes (iel) of specific pathogen free (spf) mice were compared with those of mice held without isolation and were found to differ markedly in total number and distribution of cell surface antigens. the iel from spf mice expressed significantly less thy-1, lyt-1, and lyt-2 antigens than their conventional counterparts. the local cell-mediated immune response of mucosal lymphocytes to an enteric murine coronavirus (mhv-y) was studied in inbred strains of naive sp ... | 1986 | 3005395 |
| isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. | a porcine respiratory, non-enteric virus which is related to the coronavirus transmissible gastroenteritis virus (tgev) has been isolated in pigs and in cell culture. the isolate was designated tlm 83. it has become very widespread and enzootic among the swine population in belgium and in other swine raising countries. it causes an infection of the lungs and appears to spread by aerogenic route. it does not replicate in the enteric tract. the experimental infection in conventional and gnotobioti ... | 1986 | 3018993 |
| sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes. | the 3' end of the 20-kb genome of the mebus strain of bovine enteric coronavirus (bcv) was copied into cdna and cloned into the psti site of the puc9 vector. four clones from the 3' end of the genome were sequenced either completely or in part to determine the sequence of the first 2451 bases. within this sequence were identified, in order, a 3'-noncoding region of 291 bases, the gene for a 448-amino acid nucleocapsid protein (n) having a molecular weight of 49,379, and the gene for a 230-amino ... | 1987 | 3029965 |
| shedding of enteric coronavirus in adult cattle. | electron microscopic examination of fecal specimens from adult dairy cows indicated seasonal coronavirus shedding. fifty-two cows from a 300 cow herd were monitored for shedding of coronavirus. approximately 50% to 60% of the cows monitored shed coronavirus during the winter months (november to march) of the first year of the study. during 2 subsequent years of monitoring the same cows, 20% to 30% of the cows shed coronavirus during the winter months. virus shedding was not detected during the s ... | 1987 | 3032023 |
| comparative properties of feline coronaviruses in vitro. | two feline coronaviruses were characterized to determine their biological properties in vitro and their antigenic relatedness to a previously recognized feline infectious peritonitis virus and canine coronavirus. the viruses, designated wsu 79-1146 and wsu 79-1683, were shown to have comparable growth curves with the prototype feline infectious peritonitis virus. treatment of the feline infectious peritonitis virus strains with 0.25% trypsin indicated that they were relatively resistant to prote ... | 1987 | 3038290 |
| biotinylated and radioactive cdna probes in the detection by hybridization of bovine enteric coronavirus. | cdna, synthesized on bovine coronavirus (bcv) genomic rna templates, could be used to detect very small quantities (i.e. 1 pg) of viral rna by hybridization with either radioisotopic-labelled or biotinylated recombinant plasmids. virus was optimally attached to nitrocellulose membranes when spotted in 1 x ssc, whereas 20 x ssc was superior for viral rna. denaturation and rna fixation of both rna, still encapsidated in virus particles and isolated genomic rna, was achieved by baking of the blots ... | 1988 | 3221884 |
| identification of viral antigens that induce antibody responses on exposure to coronaviruses. | various techniques were used to look for protective, non-cross-reactive antibodies in the sera of cats exposed to virulent feline infectious peritonitis virus (fipv). antibodies reactive with feline enteric coronavirus (fecv) from fipv-exposed cats were adsorbed by several passages over an fecv-sepharose column. in an elisa against fecv and fipv, the activity against both viruses was removed at the same rate; thus, no fipv-specific antibodies could be identified. by gel electrophoresis-derived e ... | 1988 | 3223654 |
| characteristics of australian human enteric coronavirus-like particles: comparison with human respiratory coronavirus 229e and duodenal brush border vesicles. | the polypeptide profiles of highly purified coronavirus-like particles (cvlps) proved to be very different from that of human respiratory coronavirus 229e and showed the particles not to be coronaviruses. differences in polypeptide profiles and morphology between the cvlps and duodenal brush border vesicles suggested that the cvlps were also not such vesicles. although they shared some basic overall similarity, the polypeptide profiles of three different but possibly antigenically identical cvlp ... | 1987 | 3426398 |
| antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins. | the antigenic relationships among seven feline coronavirus isolates were investigated by using a panel of 26 monoclonal antibodies (mabs). the mabs were categorized into five immunoreactive groups which were used to delineate two antigenic types of feline coronaviruses. one antigenic type included the more virulent feline infectious peritonitis virus (fipv) isolates (fipv-ucd-1, fipv-ucd-4, fipv-tn406, fipv-df2, and fipv-79-1146), whereas the second antigenic type was composed of the avirulent i ... | 1987 | 3599183 |
| functional differences in the peplomer glycoproteins of feline coronavirus isolates. | the feline coronaviruses can be divided into two distinct antigenic groups on the basis of antigenic differences found on the peplomer (e2) glycoprotein of the virus. because the e2 glycoprotein is responsible for many of the biological functions of coronaviruses, experiments were done to determine whether there were any e2 functional differences between these two antigenic groups. the avirulent feline infectious peritonitis virus (fipv) isolate fipv-ucd-2, which has one antigenic type of e2, wa ... | 1987 | 3599185 |
| antigenic and biological diversity of feline coronaviruses: feline infectious peritonitis and feline enteritis virus. | antigenically related feline coronaviruses cause two distinct disease manifestations in infected cats. the diseases are feline infectious peritonitis (fip), in which the virus is widely disseminated, and feline enteric coronavirus (fecv), a mild disease in which the virus is usually limited to the villi. these two viruses were found to differ in their growth in cell culture. fipv grows to higher titer, forms larger plaques and switches off host cell protein synthesis more effectively than fecv. ... | 1987 | 3619653 |
| [research on coronaviruses in water. i. adsorption and elution of the coronavirus on glass powder]. | the purpose of the research was to determine under what conditions enteric coronaviruses from water can be optimally concentrated by acid-ph adsorption on glass-powder followed by alkaline-ph elution. the 14c labelled bovine enteric coronavirus was grown on hrt18 cell monolayers and purified. virus adsorption was studied in relation to ph: optimal adsorption yield--between 50 to 75% (mean value: 62%)--was obtained at ph less than or equal to 3.5. elution was made comparatively with a 0.05 m glyc ... | 1986 | 3727400 |
| antibody prevalence to human enteric coronavirus-like particles and indications of antigenic differences between particles from different areas. brief report. | antibody to human enteric coronavirus-like particles was detected in australian aborigines, but at lower levels or not at all in europeans. there were indications of antigenic differences between particles from different areas. support was provided for consideration of such particles as infectious agents. | 1986 | 3947243 |
| bovine-murine hybridoma that secretes bovine monoclonal antibody of defined specificity. | spleen cells from a calf hyperimmunized with bovine enteric coronavirus were fused with nonproducer mouse plasmacytoma cells. stable hybridoma lines secreting bovine immunoglobulins were obtained. one line secreted monoclonal bovine immunoglobulin g2, which reacted specifically with bovine enteric coronavirus in an enzyme-linked immunosorbent assay, inhibited virus hemagglutination, and precipitated a structural polypeptide with a molecular weight of 26,000 daltons. | 1985 | 3994108 |
| oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus oc43. | virion rnas from the bovine enteric coronavirus and the human respiratory coronavirus oc43 were compared by one dimensional gel electrophoresis and by oligonucleotide fingerprinting. for each virus, approximately 55 per cent of the rna migrated as a 6.8 md species, 10 per cent as a 0.68 md species, and 15 per cent as heterogeneous small molecular weight rna. a sequence homology of greater than 96 per cent was observed between the 6.8 md species from the two viruses. the 0.68 md rna is apparently ... | 1985 | 4038068 |
| coronaviruses and gastroenteritis: evidence of antigenic relatedness between human enteric coronavirus strains and human coronavirus oc43. | a peculiar pattern of antibody response to human coronavirus (hcv) oc43 and neonatal calf diarrhea coronavirus (ncdcv) was observed in a significantly higher proportion of children with gastroenteritis (ge) than controls. it was directed only to oc43 surface antigens and appeared to be quite different form that observed in oc43 respiratory infections, in which an antibody response to both oc43 and ncdcv surface and internal antigens was found. subsequently, fecal excretion of cv-like particles w ... | 1984 | 6083436 |
| pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. | two feline coronavirus isolates were characterized by their disease-causing potential in cats. the 79-1683 feline coronavirus isolate caused an inapparent-to-mild enteritis when given oronasally to specific-pathogen-free kittens and was not a cause of feline infectious peritonitis (fip). target tissues for the virus were the mature apical epithelium of the small intestine, mesenteric lymph nodes, tonsils, thymus, and (to a lesser extent) the lungs. inoculated kittens shed high numbers of virus i ... | 1984 | 6084432 |
| coronavirus infection of the bovine respiratory tract. | two viruses, morphologically resembling coronaviruses and antigenically indistinguishable from bovine enteric coronavirus, were isolated in bovine tracheal organ cultures from the lungs and trachea of young calves with respiratory disease. intranasal and intratracheal inoculation of these viruses into neonatal calves resulted in a predominantly upper respiratory tract infection, which was associated with the development of mild respiratory symptoms. | 1984 | 6093337 |
| utilization of monoclonal antibodies for antigenic characterization of coronaviruses. | hybrid cells secreting monoclonal antibodies against bovine enteric coronavirus (becv strain g110) were obtained by fusion between sp2/o myeloma cells and splenocytes of mice hyperimmunized with purified virus. specificity of 8 from the 12 monoclonal antibodies was established to be anti-gp105 by immunochemical staining of viral polypeptides and by immunoprecipitation of radioactive-labelled viral proteins. the reactivity of three becv isolates (strain g110, f15 and ncdcv) with the different mon ... | 1983 | 6203462 |
| antigenic and polypeptide structure of bovine enteric coronavirus as defined by monoclonal antibodies. | | 1984 | 6204516 |
| experimental infection of pigs with a new porcine enteric coronavirus, cv 777. | cesarean-derived colostrum-deprived and conventionally reared pigs were orally inoculated with the coronavirus-like agent, cv 777, isolate from an outbreak of epizootic diarrhea in swine of all ages. viral particles detected by electron microscopy in the feces and intestinal contents of inoculated pigs had the typical coronavirus morphology. the present studies provided further evidence that this coronavirus-like agent is different from the two known porcine coronaviruses, transmissible gastroen ... | 1980 | 6245603 |
| an enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. | an enteric coronavirus that is antigenically closely related to feline infectious peritonitis virus (fipv) is ubiquitous in the cat population. this virus has been designated feline enteric coronavirus to differentiate it from fipv. the virus is shed in the feces by many seropositive cats; in catteries it is a cause of inapparent to mildly severe enteritis in kittens 6 to 12 weeks of age. the virus may produce a more severe enteritis in young specific-pathogen-free kittens. feline enteric corona ... | 1981 | 6267960 |
| [adaptation of an isolate of bovine enteritic coronavirus to serial growth in cell culture and characterization of the virus (author's transl)]. | coronaviruses are known to behave as pathogens in a variety of animal species. their multiplication in the differentiated enterocytes of the intestinal tract induces a severe diarrheic syndrome which may lead to death. to study these viruses and to progress in the development of prophylactic and diagnostic methods, it is essential to succeed in adapting the agents to serial growth in cell culture. in spite of several attempts adaptation to primary cell cultures could be obtained in the past only ... | 1981 | 6284011 |
| infection of a calf with the enteric coronavirus strain paris. | a tissue-culture-grown enteric coronavirus infected the whole of the gastrointestinal tract and caused enteritis and diarrhoea in a 10-day-old gnotobiotic friesian calf. diarrhoea occurred 2 days after inoculation and excretion of virus in faeces and rectal swabs increased until necropsy at 3 days. virus growth, as detected by indirect immunofluorescence, virus isolation, and thin section electron microscopy, was most extensive in the epithelium of the colon and rectum at 3 days after inoculatio ... | 1982 | 6293417 |
| comparison of different antigen preparations as substrates for use in passive hemagglutination and enzyme-linked immunosorbent assays for detection of antibody against bovine enteric coronavirus. | purified coronavirus, detergent extracts of purified coronavirus, and virus-infected madin-darby bovine kidney cells were evaluated as antigen substrates in enzyme-linked immunosorbent assay (elisa) and passive hemagglutination systems. only detergent-extracted and -unextracted, purified viruses were reactive as antigen substrates in elisa, whereas all three antigen preparations could be used for sensitization of erythrocytes in the passive hemagglutination assay. the passive hemagglutination sy ... | 1983 | 6309897 |
| monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus. | monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. similar levels of sensitivity were found for all combinations tested. a sensitive, highly specific, and reproducible assay for the detection of bovine enteric coronavirus was developed, using a mixture of two of these monoclonal antibodies reactive with antigenic c ... | 1984 | 6325490 |
| coronavirus replication in the intestinal and respiratory tracts during infection of calves. | coronaviruses have been isolated from cases of neonatal calf diarrhoea in many parts of the world and were revealed in the respiratory tract recently, during a study of calf pneumonia. in experiments on gnotobiotic calves an enteric coronavirus replicated in the nasal epithelium and a respiratory isolate was shed in faeces. the enteric coronavirus prevented subsequent infection with either homologous enteric or the respiratory isolate. a dual tropism for this agent was emphasized by the detectio ... | 1983 | 6329061 |
| plaque assay, polypeptide composition and immunochemistry of feline infectious peritonitis virus and feline enteric coronavirus isolates. | | 1984 | 6331106 |
| antigenic relatedness of human enteric coronavirus strains to human coronavirus oc43: a preliminary report. | | 1984 | 6491374 |
| passive immunity to transmissible gastroenteritis virus: intramammary viral inoculation of sows. | sows were injected intramammarily with live-attenuated tge virus, an enteric coronavirus--one sow during pregnancy and three sows during lactation. all sows were tge antibody seronegative prior to inoculation except for one naturally infected sow inoculated during lactation. the animal injected during pregnancy had primarily igg tge antibodies in milk from all glands. by contrast, sows injected during lactation had iga and igm initially, and later iga and igg tge antibodies in milk from injected ... | 1983 | 6575719 |
| [a cell line particularly susceptible to bovine enteric coronavirus replication: hrt 18 cells]. | bovine enteric corona virus (bec) multiplies spontaneously with very high efficiency in hrt 18 cells; this line established from human rectum adenocarcinoma presents some properties of the differentiated brush border cell of the intestinal villi. titers obtained are very high (5 x 10(7) tcid 50/ml virus strain f15). the virus produced had the characteristic of bec: shape, spikes, density 1.19, hemadsorption and hemagglutination of rat erythrocytes. human and dog enteritic corona viruses replicat ... | 1980 | 6768468 |
| [isolation of cell hybrids secreting specific antibodies against bovine enteric coronavirus (author's transl)]. | twelve hybrid cell lines secreting monoclonal immunoglobulins (igg) specific of bovine enteric coronavirus (bec strain g110) have been obtained following fusion of non secreting variant of the myeloma p3x63ag8, sp2o, which splenic lymphocytes of mice immunized against this virus. antibodies thus obtained have been studied by indirect immunofluorescence and seroneutralization with cells infected by two french strains g110 and f15 and an american strain of coronavirus: ncdcv. immunochemical studie ... | 1982 | 6807513 |
| coronavirus-like particles as aetiological agents of acute non-bacterial gastroenteritis in humans. | the morphology of coronavirus-like particles found in faecal extracts of neonates and infants with acute gastroenteritis show similarities with the structure of human coronaviruses. these coronavirus-like particles can be detected in faeces of sick neonates and infants in higher frequency than in healthy children of the same age, their association with this clinical syndrome is discussed. the significance of enteric coronavirus-like particles in acute gastroenteritis of adults is questionable si ... | 1983 | 6873473 |
| bovine enteric coronavirus structure as studied by a freeze-drying technique. | a strain of bovine coronavirus (f15) was studied by electron microscopy using a freeze-drying technique. purified coronavirus preparations show three different categories of image: (i) 'blackberry-like' virions, (ii) virions with a smooth depression at their surface, and (iii) apparently broken particles showing very clearly the areas of spike insertion in the virus membrane. virus projections resemble 'mushrooms' with the 'stalk' inserted at the virus membrane. a model of the virion structure i ... | 1982 | 6890987 |
| in vivo morphogenesis of a new porcine enteric coronavirus, cv 777. | | 1981 | 7247728 |
| plaque assay for titration of bovine enteric coronavirus. | the plaquing ability of two isolates of bovine enteric coronavirus (becv) was studied in hrt18 (human rectal adenocarcinoma) cell monolayers. both isolates were able to induce plaque formation within 2 to 3 days; plaques appeared as round opalescent areas which remained colourless after neutral red or crystal violet staining. a good correlation was found between the titres as determined either by counting the plaques that were visible to the naked eye before and after neutral red staining, or by ... | 1981 | 7310384 |
| hemagglutination and structural polypeptides of a new coronavirus associated with diarrhea in infant mice. | the hemagglutination (ha) and receptor destroying enzyme (rde) activities of a newly isolated mouse enteric coronavirus (designated as dvim) are described. dvim agglutinates mouse or rat red blood cells (rbc) at 4 degrees c. at 37 degrees c the agglutination was rapidly reversed. the optimal ph for ha and for rde activities using mouse red cells were shown to be 6.5 and 7.3 respectively. hemagglutination by dvim was not inhibited by pretreatment of rbcs with vibrio cholerae filtrate or by pretre ... | 1980 | 7436741 |
| site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity. | the pathogenicity of neutralization-resistant mutants of the enteric coronavirus transmissible gastroenteritis virus (tgev) was examined in the newborn piglet. the parental virus (purdue-115 strain), as well as several mutants selected using monoclonal antibodies (mabs) directed to antigenic sites a and b, caused an acute enteritis with 100% mortality. by contrast, most of the site d (mab 40.1) mutants exhibited a strongly reduced enteropathogenicity, leading to the survival of animals inoculate ... | 1995 | 7561760 |
| enhancement and neutralization of feline infectious peritonitis virus infection in feline macrophages by neutralizing monoclonal antibodies recognizing different epitopes. | the interaction between the enhancing and neutralizing activities of three monoclonal antibodies (mabs) (5-6-2, 6-4-2 and 7-4-1) to the spike protein of feline infectious peritonitis virus (fipv) strain 79-1146 was determined using feline macrophages. at a high mab concentration, all of the three mabs completely inhibited the fipv infection at 37 c. however, two of them (6-4-2 and 7-4-1) enhanced fipv infection when either the mab concentration or reaction temperature was lowered. these mabs als ... | 1993 | 7694052 |
| serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides and e. coli fusion protein. | cats exposed to feline infectious peritonitis virus (fipv) or feline enteric coronavirus (fecv) cannot be differentiated by serological analysis. three synthetic peptides and an e. coli recombinant fusion protein generated from fipv 79-1146 spike gene sequence were produced. coronavirus positive cat sera reacted to peptide aa 950-990 but were non-reactive to aa137-151 and aa 150-180 peptides as demonstrated by elisa. amino acid sequence 97-222 expressed as a galk fusion protein in e. coli was te ... | 1995 | 7794112 |
| detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction. | feline coronavirus infections in cell cultures and in fresh and fixed feline tissues were detected using a polymerase chain reaction (pcr) test. cell cultures were inoculated with feline infectious peritonitis virus (fipv), feline enteric coronavirus (fecv) or sham inoculum. the tissue samples of liver, kidney and spleen were taken from specific-pathogen-free (spf) cats that were inoculated intranasally with 10(3) tcid50 of fipv 79-1146 (n = 10), fipv ucd1 (n = 3) or sham inoculum (n = 3), from ... | 1994 | 7839586 |
| biological and genetic characterization of a hemagglutinating coronavirus isolated from a diarrhoeic child. | the coronavirus strain hecv-4408 was isolated from diarrhea fluid of a 6-year-old child with acute diarrhea and propagated in human rectal tumor (hrt-18) cells. electron microscopy revealed coronavirus particles in the diarrhea fluid sample and the infected hrt-18 cell cultures. this virus possessed hemagglutinating and acetylesterase activities and caused cytopathic effects in hrt-18 cells but not in mdbk, gbk and fe cells. one of four s-specific monoclonal antibodies reacted in western blots w ... | 1994 | 7852955 |
| contribution of antibody-secreting cells induced in mucosal lymphoid tissues of pigs inoculated with respiratory or enteric strains of coronavirus to immunity against enteric coronavirus challenge. | two antigenically related porcine coronaviruses, transmissible gastroenteritis virus (tgev) which infects primarily the intestinal tract and causes severe diarrhea, and porcine respiratory coronavirus (prcv) which infects the respiratory tract and causes subclinical or mild respiratory infections, presented a unique opportunity to study the interrelationship of gut-(galt) and bronchus-associated lymphoid tissues (balt) and their contribution to protective immunity against tgev infection. pigs we ... | 1994 | 8144965 |
| genomic organization and expression of the 3' end of the canine and feline enteric coronaviruses. | the genomic organization at the 3' end of canine coronavirus (ccv) and feline enteric coronavirus (fecv) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (fipv) and transmissible gastroenteritis virus (tgev) of swine. comparison of the latter two has previously revealed an extra open reading frame (orf) at the 3' end of the fipv genome, lacking in tgev, now designated orf 6b. both ccv and fecv possess 6b-related orfs. the ccv orf 6b is colinear with ... | 1993 | 8209715 |
| cloning and sequence analysis of the spike gene from several feline coronaviruses. | the dna sequence encoding the spike gene from the df2 strain of type ii feline infectious peritonitis virus (fipv), a temperature sensitive fipv virus (ts-df2) and an isolate of feline enteric coronavirus (fecv 1683) were determined. comparison of the published wsu 1146 and df2 fipv s genes showed that the viruses shared a high degree of homology (99.6%). likewise, the s gene of the virulent df2 fipv virus was closely conserved to that isolated from the vaccine virus strain, ts-df2 fipv. in cont ... | 1993 | 8209725 |
| transmissible gastroenteritis virus and porcine respiratory coronavirus: molecular characterization of the s gene using cdna probes and nucleotide sequence analysis. | two transmissible gastroenteritis virus (tgev, miller strain) cdna clones were identified and their nucleotide sequences determined. the clones were non-overlapping and were located in the 5' region of the s glycoprotein gene. the tgev clone pe21 contained 381 bp of the s glycoprotein gene and had > 98% nucleotide and amino acid sequence homology with the purdue (p115) strain of tgev and over 87% sequence homology with feline infectious peritonitis virus (fipv). the tgev clone, pd24, contained 2 ... | 1993 | 8209764 |
| characterization of the nonstructural and spike proteins of the human respiratory coronavirus oc43: comparison with bovine enteric coronavirus. | the nucleotide sequence of the region between the spike (s) and the membrane (m) protein genes, and sequences of the s and ns2 genes of the oc43 strain of human coronavirus (hcv-oc43) were determined. the ns2 gene comprises an open reading frame (orf) encoding a putative nonstructural (ns) protein of 279 amino acids with a predicted molecular mass of 32-kda. the s gene comprises an orf encoding a protein of 1353 amino acid residues, with a predicted molecular weight of 149,918. sequence comparis ... | 1993 | 8209772 |
| characterization of monoclonal antibodies to bovine enteric coronavirus and antigenic variability among quebec isolates. | twenty monoclonal antibodies (mabs) were prepared against the mebus strain of bovine enteric coronavirus, 14 of them reacting with the peplomeric s (gp 100) glycoprotein. competition binding assays allowed the definition of at least 4 distinct antigenic domains for the s glycoprotein, designated as a, b, c, and d; epitopes associated to neutralizing activity being located in sites a, b, and c. one mab directed to the hemagglutinin he (gp 140/gp 65) glycoprotein inhibited the hemagglutinating act ... | 1993 | 8347084 |
| detection of feline infectious peritonitis virus infection in cell cultures and peripheral blood mononuclear leukocytes of experimentally infected cats using a biotinylated cdna probe. | a dot blot hybridization assay, using a biotinylated cdna probe, was able to detect feline infectious peritonitis virus (fipv) rna in felis catus whole fetus (fcwf-4) cells infected with the fipv isolates df2, 79-1146, ucd1, and ucd2. the probe cross-hybridized in the dot blot assay with nucleic acid of a closely related feline coronavirus, feline enteric coronavirus (fevc)-79-1683. to construct the probe, a 2.5 kilobase cdna, prepared from fipv-df2 genomic rna, was molecularly cloned. the recom ... | 1993 | 8384740 |
| porcine respiratory coronavirus: molecular features and virus-host interactions. | since 1984, a previously unrecognized respiratory coronavirus, causing a mostly unapparent infection, has rapidly and massively spread within the swine population in europe, and few years later, a virus with similar characteristics has been identified in the usa. the agent, designated prcv, appears to be derived from the porcine enteric coronavirus tgev. the aim of the present article is to review comprehensively the state of the knowledge about this new virus and its infection. the review inclu ... | 1993 | 8393722 |
| seroprevalence of infectious disease agents in free-ranging florida panthers (felis concolor coryi). | serum samples obtained from 38 free-ranging florida panthers (felis concolor coryi) in southern florida, march 1978 through february 1991, were tested for antibodies against eight bacterial, parasitic, and viral disease agents. sera were positive for antibodies against feline panleukopenia virus (fpv) (78%), feline calicivirus (56%), feline immunodeficiency virus/puma lentivirus (37%), feline enteric coronavirus/feline infectious peritonitis virus (19%), and toxoplasma gondii (9%). all samples w ... | 1993 | 8445789 |
| biphasic immune responses of cats under controlled infection with a feline enteric coronavirus-79-1683 strain. | kittens inoculated orally with 10(2) pfu of feline enteric coronavirus developed no antibody to the virus despite the repeated challenges. however, they developed antibody for a long period with 5 x 10(3)-1.6 x 10(5) (mean 3 x 10(4)) and with 2.5 x 10(3)-2 x 10(4) (mean 6 x 10(3)) immunoperoxidase antibody titer when they were challenged with 10(5) and 10(3) pfu of virus following previous challenges, respectively. viremia was found when kittens were inoculated with 10(5) pfu of virus, but not w ... | 1995 | 8519920 |
| protection of cats from infectious peritonitis by vaccination with a recombinant raccoon poxvirus expressing the nucleocapsid gene of feline infectious peritonitis virus. | feline infectious peritonitis virus (fipv) is a coronavirus that induces an often fatal, systemic infection in cats. various vaccines designed to prevent fipv infection have been shown to exacerbate the disease, probably due to immune enhancement mediated by virus-specific immunoglobulins against the outer envelope (s) protein. an effective vaccine would be one that induces cell-mediated immunity without disease enhancing antibodies. in this report, we describe the use of a recombinant raccoon p ... | 1995 | 8830483 |
| cloning and expression of fecv spike gene in vaccinia virus. immunization with fecv s causes early death after fipv challenge. | the spike gene of the feline enteric coronavirus (fecv), strain fecv-1683, was pcr amplified from total rna extracted from fecv-infected cells and its sequence determined. a primary translation product of 1454 amino acids is predicted from the nucleotide sequence, containing a n-terminal signal sequence, a c-terminal transmembrane region and 33 potential n-glycosylation sites. the sequence shares 92% homology with the previously published feline infectious peritonitis virus, strain wsu-1146; how ... | 1995 | 8830486 |