Publications

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cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil.anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ...200717278086
[seroprevalence of anaplasma marginale in cattle from center-south region of paraná state, brazil by a competitive elisa test with recombinant msp5-pr1 protein].anaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the center-southern region of the paraná state, including ponta grossa, guarapuava and laranjeiras do sul municipalities. a survey of antibodies igg class against anaplasma marginale was performed through a competitive immune absorbent assay (celisa-pr1). from the 223 sera examined, 130 (58.74%) reacted to celisa-pr1 test, suggesting an region of enzootic instability, with a significant percenta ...200919500456
seroprevalence of anaplasma marginale in cattle in parana state, brazil, by msp-5 competitive elisa. 19989668503
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