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prevalence of borrelia burgdorferi s.i. in ixodes ricinus ticks from styria (austria) and species identification by pcr-rflp analysis.a total of 1163 i. ricinus ticks were collected in 3 different regions (15 localities) in styria (austria) in june 1997 and examined for the presence of spirochetes by dark field microscopy. the mean infection rate was 20.8%. among 310 adults, 24.2% were positive and among 853 nymphs, 19.6% were positive. all 15 collection areas were shown to harbour infected nymphs with a positivity rate ranging from 5.8% (3/52) to 32.1% (18/56). isolation attempts in bskii medium resulted in 29 isolates. speci ...19989987185
species identification of borrelia burgdorferi sensu lato from tick and human isolates in styria (austria) by pcr-rflp analysis.seventy-one isolates of borrelia burgdorferi sensu lato (b.b.s.l.) derived from ixodes ricinus ticks (50 strains) and patients (21 strains) were characterised by pcr-rlfp analysis. in four cases the human isolates were obtained from the cerebrospinal fluid (csf) of patients with clinical symptoms of neuroborreliosis and in 17 cases from skin biopsies of patients with dermatological manifestation of lyme borreliosis. ixodes ricinus isolates originated from 14 localities in three regions (mur vall ...199910666817
prevalence of borrelia burgdorferi sensu lato in the tick ixodes ricinus in the styrian mountains of austria.a total of 691 ixodes ricinus (22 male, 39 female, 501 nymphs and 129 larvae), the tick vector of lyme borreliosis, were collected by flagging from vegetation in 11 areas at altitudes between 789 m and 1350 m above sea level in mixed woodland with pasture land (cattle) in the province of styria in austria. the ticks were individually examined for presence of borrelia burgdorferi sensu lato by dark-field microscopy and 107 of them by real-time pcr. attempts to cultivate borreliae were made in bsk ...200617160607
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