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molecular characterization of three new avian infectious bronchitis virus (ibv) strains isolated in quebec.three unrecognized field isolates of infectious bronchitis virus (ibv) were recovered from commercial broiler chickens vaccinated with live mass viral strain (h120). these isolates were identified by immunofluorescence using monoclonal antibodies produced against reference serotypes: mass, conn, and ark. rt-pcrs were performed on viral rnas to amplify s1 gene using a specific set of primers s1oligo3' and s1oligo5'. restriction polymorphism (rflp) of pcr products was determined by the use of haei ...200212206312
types of avian infectious bronchitis strains isolated in quebec.between 1976 and 1980, 24 isolates of infectious bronchitis virus were obtained from quebec flocks. the serological classification of these isolates was demonstrated by cross neutralization tests using antisera to 13 different reference virus strains. of the 24 isolates, ten were identified as connecticut, six holland and one se-17 types. seven strains did not react with any of the specific antisera.19826284325
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