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subtype assignment and phylogenetic analysis of hiv type 1 strains in patients from swaziland.aim of this study was to assess hiv-1 subtype distribution and prevalence of transmitted mutations related to antiretroviral drugs in swaziland. according to the who guidelines, 47 plasma samples from naive patients stored at hiv/aids national reference laboratory in mbabane between 2002 and 2003, before the introduction of antiretroviral therapy in the country, were studied. hiv-1 rna was extracted from the plasma samples, rt and protease regions of pol gene were amplified and sequenced. the mu ...200818257688
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