| application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. | the rapid, indirect enzyme-labeled antibody (ela) microplate test has been developed as a diagnostic and surveillance tool to aid in the control of animal disease. the test has been applied to viral (hog cholera), parasitic (trichinosis), and bacterial (brucellosis) diseases of animals. a correlation of greater than 95% was observed between the hog cholera ela test and the serum neutralization test for hog cholera in greater than 2,000 field samples obtained during the 1976 epizootic in new jers ... | 1977 | 409789 |
| effects of stage of gestation and breed on bovine responses to vaccination with brucella abortus strain 19. | eighty-eight cattle were injected sc with 2.5 x 10(8) viable cells of brucella abortus strain 19. all but 1 heifer became seropositive on the basis of the results of 7 brucellosis tests, and the proportion positive decreased with time. the proportion of cattle that were seropositive during a 20- to 67-week period after vaccination was as follows, in decreasing order: hemolysis-in-gel, 59%; buffered-acid plate antigen, 39%; elisa, 16%; card, 10%; rivanol, 8%; cold complement-fixation, 7%; and aut ... | 1991 | 1769876 |
| vaccination of cattle against brucellosis using either a reduced dose of strain 19 or one or two doses of 45/20 vaccine. | three groups, each of 14 mature jersey heifers, were vaccinated. they were mated about 2 months later and those that became pregnant were challenged at about 6.5 months of pregnancy by the conjunctival application of virulent brucella abortus. group 1 heifers received 2 doses of b. abortus 45/20 vaccine 2 months apart. only 5 of the 14 heifers became pregnant, and of these 5 only one resisted challenge. group 2 heifers received only one dose of 45/20 vaccine, 5 of the 10 challenged resisted infe ... | 1983 | 6626063 |
| vaccination of heifers with a reduced dose of brucella abortus strain 19 vaccine before first mating. | ten jersey heifers aged 14 to 23 months were vaccinated with 2.25 x 10(8) cells of living brucella abortus strain 19 vaccine. they and 10 similar non-vaccinated heifers were subsequently mated and when about 6 months pregnant were challenged by the conjunctival application of a virulent culture of b. abortus. the serological responses to vaccination was much less than is usually seen following vaccination with the normal dose of strain 19, especially when the indirect haemolysis test was used. a ... | 1981 | 7340776 |