| [stratification of a hyperendemic city in hemorrhagic dengue]. | any effort to control dengue hemorrhagic fever (dhf) faces a number of challenges. among these are the great environmental heterogeneity of homes and neighborhoods in urban centers where the primary dengue vector, aedes aegypti, breeds, as well as shortages of resources and of personnel trained in mosquito control. adequate epidemiological surveillance could serve as a basis to begin to stratify urban communities and identify the areas in them where control efforts should be focused. in this stu ... | 2000 | 11141964 |
| detection of dengue viruses in field-caught aedes aegypti (diptera: culicidae) in maracay, aragua state, venezuela by type-specific polymerase chain reaction. | virological surveillance of dengue viruses in aedes aegypti populations constitutes a powerful tool for early prediction of dengue outbreaks. we have standardized a protocol for viral rna extraction from individual and pools of mosquitoes that permits a sensitive detection of dengue virus without rna degradation or pcr inhibition when we apply a semi-nested rt-pcr. the limit of detection for each dengue serotype was 0.1 pfu. in a prospective field study conducted from november 2000 to december 2 ... | 2005 | 15639750 |