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flock-prevalence of border disease virus infection in basque dairy-sheep estimated by bulk-tank milk analysis.bulk-tank milk (btm) samples from 154 sheep flocks were used to estimate bdv prevalence in the basque country in spain using an elisa and a rt-pcr test. the proportion of antibody-positive flocks was 68% but varied significantly between provinces and was 93% in araba and 54-55% in bizkaia and gipuzkoa. most elisa-positive flocks had very low antibody inhibition percentage (aip) indicating high seroprevalence and recent bdv exposure. however, only 9% flocks were pcr-positive suggesting few infect ...200616979308
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