| isolation of avian pneumovirus from an outbreak of respiratory illness in minnesota turkeys. | antibodies to avian pneumovirus (apv) were first detected in minnesota turkeys in 1997. virus isolation was attempted on 32 samples (28 tracheal swabs, 4 pools of trachea and turbinates) that were positive for apv by reverse transcriptase polymerase chain reaction (rt-pcr). the cell cultures used were chicken embryo fibroblast (cef), vero cells, and qt-35 cells. five virus isolates were obtained from these samples, and the identity of the isolates was confirmed by rt-pcr. four isolates were obta ... | 2000 | 10730950 |
| avian pneumovirus infection in minnesota turkeys: experimental reproduction of the disease. | avian pneumovirus (apv) is an emerging viral respiratory disease agent of turkeys in minnesota. clinical signs of apv infection include open mouth breathing, ocular and nasal discharge, and swelling of infraorbital sinuses. the virus spreads rapidly among flocks of susceptible turkeys and is associated with increased mortality rates. a flock of 11-wk-old turkeys experienced a respiratory problem characterized by coughing, sneezing, swollen sinuses, and nasal discharge. the reverse transcriptase- ... | 2000 | 10737667 |
| experimental and field evaluation of a live vaccine against avian pneumovirus. | the attenuation of an avian pneumovirus (apv) isolate (apv/mn/turkey/1-a/97) by 63 serial passages in cell culture (seven in chicken embryo fibroblasts and 56 in vero cells) and its evaluation as a live attenuated vaccine in turkey poults is described. the birds were vaccinated with two different doses of attenuated virus (10(4.5) median tissue culture infectious dose (tcid(50))/ml and 10(2.5) tcid(50) /ml) at 2 weeks of age, and were challenged 2 weeks later with virulent apv. no clinical signs ... | 2002 | 12396339 |