Publications

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endophytic bacteria in coffea arabica l.eighty-seven culturable endophytic bacterial isolates in 19 genera were obtained from coffee plants collected in colombia (n = 67), hawaii (n = 17), and mexico (n = 3). both gram positive and gram negative bacteria were isolated, with a greater percentage (68%) being gram negative. tissues yielding bacterial endophytes included adult plant leaves, various parts of the berry (e.g., crown, pulp, peduncle and seed), and leaves, stems, and roots of seedlings. some of the bacteria also occurred as ep ...200516187260
penicillium species endophytic in coffee plants and ochratoxin a production.tissues from coffea arabica, c. congensis, c. dewevrei and c. liberica collected in colombia, hawaii and at a local plant nursery in maryland were sampled for the presence of fungal endophytes. surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. dna was extracted from a set of isolates visually recognized as penicillium, and the internal transcribed spacer region and partial lsu-rdna was amplified and sequenced. comparison of dna sequ ...200616800302
flight activity and field infestation relationships for coffee berry borer in commercial coffee plantations in kona and kau districts, hawaii.the coffee berry borer (cbb), hypothenemus hampei ferrari (coleoptera: curculionidae: scolytinae) is a recent invader to hawaii. to date, limited information regarding the seasonal phenology of this pest on the islands limits the implementation of integrated control strategies. as part of a coffee farmer training program, we monitored cbb flight activity in 15 coffee plantations (kona and kau districts) over 10 mo with methanol-ethanol (3:1 ratio) baited traps. concurrently, we quantified cbb in ...201729029300
penicillium coffeae, a new endophytic species isolated from a coffee plant and its phylogenetic relationship to p. fellutanum, p. thiersii and p. brocae based on parsimony analysis of multilocus dna sequences.penicillium coffeae is described as a novel endophyte isolated from a coffea arabica l. plant in hawaii. the species is slow growing with short, vesiculate, monoverticillate conidiophores. phylogenetic analysis using three loci shows that p. coffeae forms a strongly supported clade with p. fellutanum, p. charlesii, p. chermesinum, p. indicum, p. phoeniceum and p. brocae. phenotypic ally these species are quite similar but can be distinguished. the ef-1alpha gene from p. fellutanum, p. charlesii, ...200616392254
efficacy of traps, lures, and repellents for xylosandrus compactus (coleoptera: curculionidae) and other ambrosia beetles on coffea arabica plantations and acacia koa nurseries in hawaii.the black twig borer, xylosandrus compactus (eichhoff) (coleoptera: curculionidae: scolytinae), is a pest of coffee and many endemic hawaiian plants. traps baited with chemical attractants commonly are used to capture ambrosia beetles for purposes of monitoring, studying population dynamics, predicting outbreaks, and mass trapping to reduce damage. the objectives of this research were to optimize trapping systems for x. compactus and other ambrosia beetles such as xylosandrus crassiusculus (mots ...201222525068
dispersion and sequential sampling plan for xylosandrus compactus (coleoptera: curculionidae) infesting hawaii coffee plantations.the black twig borer, xylosandrus compactus (eichhoff) (coleoptera: curculionidae: scolytinae), is a serious pest of coffee (coffea arabica l.) in the kona region of the island of hawaii, the center of the largest area of coffee production within the state of hawaii. this study indirectly characterizes the spatial distribution of x. compactus in coffee plantations through assessment of twig borer damage, and presents a sequential sampling plan for monitoring x. compactus population densities. ta ...201323575018
response of female ceratitis capitata (diptera: tephritidae) to a spinosad bait and polymer matrix mixture with extended residual effect in hawaii.the effectiveness of foliar applications of protein baits against pestiferous fruit flies (tephritidae) can be adversely affected by a rapid loss of attractive volatile compounds and by rainfall due to the high water solubility of the baits. in a large coffee, coffea arabica l., plantation in hawaii with high and low populations of mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae), the relative attractiveness of gf-120 nf naturalyte fruit fly bait as either a 40% (vo ...201122299345
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