| geographical clustering of trypanosoma cruzi i groups from colombia revealed by low-stringency single specific primer-pcr of the intergenic regions of spliced-leader genes. | a low-stringency single-primer polymerase chain reaction (lssp-pcr) typing procedure targeted to the intergenic regions of spliced-leader genes (sl) was designed to profile trypanosoma cruzi i stocks from endemic regions of colombia. comparison between sl-lssp-pcr profiles of parasite dna from vector faeces and cultures isolated from those faeces showed more conservative signatures than profiles using lssp-pcr targeted to the minicircle variable regions (kdna). this was also observed by analysin ... | 2009 | 18850114 |