| discovery of an enzootic cycle of borrelia burgdorferi in neotoma mexicana and ixodes spinipalpis from northern colorado, an area where lyme disease is nonendemic. | an intensive enzootic cycle of borrelia burgdorferi was seen in populations of the mexican wood rat, neotoma mexicana, and ixodes spinipalpis ticks in northern colorado. cultures of rodent ear tissue and ticks yielded 63 spirochetal isolates: 38 n. mexicana, 2 peromyscus difficilis, and 23 i. spinipalpis. all 63 isolates were identified as b. burgdorferi sensu lato by polymerase chain reaction; a representative subset was characterized as b. burgdorferi by sds-page and immunoblotting. a tick-der ... | 1994 | 8077722 |
| culturing selects for specific genotypes of borrelia burgdorferi in an enzootic cycle in colorado. | in colorado, borrelia burgdorferi sensu stricto, the etiologic agent of lyme disease, is maintained in an enzootic cycle between ixodes spinipalpis ticks and neotoma mexicana rats (27). the frequencies of flagellin (fla), 66-kda protein (p66), and outer surface protein a (ospa) alleles were examined in 71 b. burgdorferi isolates from samples from colorado. approximately two-thirds of these samples were isolates from i. spinipalpis ticks that had been cultured in bsk-h medium prior to dna extract ... | 1997 | 9276416 |
| an interstate outbreak of tick-borne relapsing fever among vacationers at a rocky mountain cabin. | in july 1995, an outbreak of acute febrile illness affected 11 (48%) of 23 family members from nebraska and kansas who had vacationed at a colorado cabin in june. similar symptoms were identified among five (17%) of 30 additional persons from nebraska, kansas, florida, and texas who had vacationed at the same cabin. symptoms suggested tick-borne relapsing fever (tbrf). although no spirochetes were detected in available blood smears from five case-patients, borrelia hermsii was cultured from the ... | 1998 | 9660457 |
| population genetics and phylogenetic analysis of colorado borrelia burgdorferi. | borrelia burgdorferi is transmitted in an enzootic cycle in colorado between the tick ixodes spinipalpis and the woodrat neotoma mexicana. the genetic relationship of colorado isolates to other b. burgdorferi isolates is unknown nor have relationships among various colorado isolates been determined. portions of the flagellin (fla), 66-kd protein, and outer surface protein a (ospa) genes were amplified from 71 colorado isolates, screened for genetic variability using single strand conformation po ... | 1999 | 10348251 |
| transmission of the agent of human granulocytic ehrlichiosis by ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with borrelia burgdorferi in northern colorado. | previous work described an enzootic cycle of borrelia burgdorferi sensu lato (hereafter referred to as b. burgdorferi) maintained by the rodent neotoma mexicana and the tick ixodes spinipalpis in northern colorado. we investigated the incidence of coinfection among rodents with the agent of human granulocytic ehrlichiosis (aohge). aohge was detected in 23.5% of 119 rodent spleens examined. biopsy results indicated that 78 (65.5%) of the 119 rodents were positive for b. burgdorferi, whereas 22 (7 ... | 2000 | 10915099 |
| borrelia isolates in northern colorado identified as borrelia bissettii. | previous work described borrelia burgdorferi sensu lato group dn127 as a new genospecies, borrelia bissettii, and prompted the present study to identify the borrelia spp. that exist in northern colorado. to determine the genospecies present, we analyzed two specific intergenic spacer regions located between the 5s and 23s and the 16s and 23s ribosomal genes. phylogenetic analysis of the derived sequences clearly demonstrated that these isolates, originating from rodents captured in the foothills ... | 2000 | 10921989 |
| serologic survey for selected infectious disease agents in swift and kit foxes from the western united states. | a serologic survey of swift fox (vulpes velox) and kit fox (v. macrotis) from the western usa was conducted for 12 infectious diseases. samples from swift fox were collected between 1987 and 1992 from colorado (n = 44), kansas (n = 10), and wyoming (n = 9). samples from kit fox were collected in california (n = 86), new mexico (n = 18), utah (n = 9), and arizona (n = 6). overall antibody prevalence rates were 33 of 110 (30%) for canine parvovirus (cpv), 9 of 72 (13%) for canine distemper virus ( ... | 2000 | 11085448 |
| babesia microti and borrelia bissettii transmission by ixodes spinipalpis ticks among prairie voles, microtus ochrogaster, in colorado. | an endemic transmission cycle of babesia microti was discovered in colorado in the foothills of the rocky mountains. b. microti were found by pcr in 4 of 25 ixodes spinipalpis tick pools tested (a 3.2 % minimum infection rate) and in 87% (13 of 15) of microtus ochrogaster (the prairie vole) spleen and blood samples. using naturally infected i. spinipalpis collected from wild-caught m. ochrogaster as vectors, b. microti and borrelia bissettii were successfully transmitted to laboratory-born m. oc ... | 2000 | 11155930 |
| isolation and characterization of borrelia parkeri in ornithodoros parkeri (ixodida: argasidae) collected in colorado. | this study describes the identification of borrelia parkeri spirochetes in colorado. two isolates of b. parkeri (6230 and 6232) were recovered from ornithodoros parkeri cooley ticks collected at an inactive prairie dog town in moffat county. both isolates were partially characterized by sequencing and subsequent parsimony and neighbor-joining analyses of appropriate regions of the 16s ribosomal rna, flagellin and p66 genes. analyses of the 16s gene sequences from the colorado isolates indicated ... | 2001 | 11580039 |
| use of a sentinel host system to study the questing behavior of ixodes spinipalpis and its role in the transmission of borrelia bissettii, human granulocytic ehrlichiosis, and babesia microti. | ixodes spinipalpis maintains borrelia bissettii spirochetes in colorado in a cycle involving wood rats and deer mice. this tick has been described as nidicolous, remaining either attached to its rodent hosts or in the rodent nest. nidicolous ticks pose little risk of pathogen transmission to humans if they do not actively quest for hosts. to investigate the questing potential of i. spinipalpis, sentinel mice were placed in an area where i. spinipalpis had been commonly found on wood rats and dee ... | 2001 | 11693872 |
| novel potential reservoirs for borrelia sp. and the agent of human granulocytic ehrlichiosis in colorado. | previous work demonstrated that ixodes spinipalpis ticks maintained an enzootic cycle of borrelia bissettii and the agent of human granulocytic ehrlichiosis (aohge) within woodrats (neotoma mexicana) and deer mice (peromyscus maniculatus) in northern colorado (usa). because i. spinipalpis is the only known vector of b. bissettii and aohge in colorado, this study was designed to determine the reservoir status of other hosts of i. spinipalpis in five distinct ecological zones along the front range ... | 2002 | 12038153 |
| detection and characterization of borrelia bissettii in rodents from the central california coast. | this is the first report of borrelia burgdorferi sensu lato in rodents from san luis obispo county, with most isolates obtained from a previously unreported host, neotoma lepida thomas. b. burgdorferi sensu lato was identified in seven rodent species, including the california vole, microtus californicus peale; dusky-footed woodrat, neotoma fuscipes baird; desert woodrat, neotoma lepida thomas; brush mouse, peromyscus boylii baird; california mouse, peromyscus californicus gambel; deer mouse, per ... | 2004 | 15311469 |