| determination of hepatitis c virus genotypes in the united states by cleavase fragment length polymorphism analysis. | we describe the application of a new dna-scanning method, which has been termed cleavase fragment length polymorphism (cflp; third wave technologies, inc., madison, wis.), for the determination of the genotype of hepatitis c virus (hcv). cflp analysis results in the generation of structural fingerprints that allow discrimination of different dna sequences. we analyzed 251-bp cdna products generated by reverse transcription-pcr of the well-conserved 5'-noncoding region of hcv. we determined the g ... | 1997 | 9399512 |
| hepatitis c virus transmission in hemodialysis units: importance of infection control practices and aseptic technique. | we investigated 4 hepatitis c virus (hcv) infection outbreaks at hemodialysis units to identify practices associated with transmission. apparent failures to follow recommended infection control precautions resulted in patient-to-patient hcv transmission, through cross-contamination of the environment or intravenous medication vials. fastidious attention to aseptic technique and infection control precautions are essential to prevent hcv transmission. | 2009 | 19642900 |
| immunoassays to study prevalence of antibody against gb virus c in blood donors. | immunoassays were developed to determine the seroprevalence of antibody against human gb virus c (gbv-c). the antigenic target in each assay was a 44.6-kda glycosylated protein representing the first 315 amino acids encoded by the gbv-c e2 gene. sera or plasma were assayed for e2 antibody using an anti-human eia format in which antigen-coated polystyrene beads were reacted with sample, and bound antibody was detected by addition of enzyme labelled goat anti-human igg. the presence of anti-e2 ant ... | 1997 | 9395139 |