Publications

detection of lassa virus antinucleoprotein immunoglobulin g (igg) and igm antibodies by a simple recombinant immunoblot assay for field use.	the nucleoprotein of lassa virus, strain josiah, was expressed in escherichia coli as an n-terminally truncated, histidine-tagged recombinant protein. following affinity purification the protein was completely denatured and spotted onto nitrocellulose membrane. a total of 1 microgram of protein was applied for detection of lassa virus antibodies (lva) in a simple immunoblot assay. specific anti-lassa immunoglobulin m (igm) antibodies could be detected by increasing the amount of protein to 5 mic ...	1998	9774554
[lassa fever].	a 22-year-old female german student was admitted with fever of unknown origin for 5 days to the hospital of her hometown immediately after returning from a 7-week journey under simple conditions through 4 west african countries. after exclusion of malaria and typhoid and nonrespondence to antibiosis, she was transferred on the 4th day to the department of tropical medicine in würzburg. after the clinical assumption of lassa fever, the virus was confirmed by pcr within 3 hours (bernhard nocht ins ...	2000	10935419