Publications

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farm factors associated with the presence of mycobacterium paratuberculosis infection in dairy herds on the new york state paratuberculosis control program.an extensive questionnaire was developed and used to collect data from 33 herds that were on the new york state paratuberculosis control program, to study farm factors associated with the presence of mycobacterium paratuberculosis infection in dairy herds. the results of the last whole herd paratuberculosis fecal culture were used to indicate presence of infection in a herd, with herds having one or more animals positive classified as 'infected'. the average prevalence within herds was 5.2%. fou ...19979443331
cow-level evaluation of a kinetics elisa with multiple cutoff values to detect fecal shedding of mycobacterium avium subspecies paratuberculosis in new york state dairy cows.in control programs for mycobacterium avium subsp. paratuberculosis (map), the infection status of the cows in a herd is often obtained by testing (a sample of) the herd with an elisa that may lack some sensitivity and specificity but that is fast and inexpensive. in new york state (nys), an unabsorbed kinetics elisa (kela) has been used extensively for map control. the objective of this study was to determine the relative sensitivity and specificity of the kela for detection of fecal shedding o ...200516169619
a whole genome association analysis identifies loci associated with mycobacterium avium subsp. paratuberculosis infection status in us holstein cattle.the purpose of this study was to identify loci associated with mycobacterium avium subspecies paratuberculosis (map) infection status in us holsteins using the illumina bovinesnp50 beadchip whole genome single nucleotide polymorphism (snp) assay. two hundred forty-five cows from dairies in new york, pennsylvania and vermont enrolled in longitudinal herd studies between january 1999 and november 2007 were assessed for the presence of map in both faecal and tissue samples. an animal was considered ...200919422364
correlation between herrold egg yolk medium culture and real-time quantitative polymerase chain reaction results for mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental samples.real-time quantitative polymerase chain reaction (qpcr) testing for mycobacterium avium subspecies paratuberculosis (map) in fecal samples is a rapid alternative to culture on herrold egg yolk medium (heym), the traditional antemortem reference test for map. although the sensitivity and specificity of these 2 tests have been estimated based on dichotomized test results, the correlation between real-time qpcr threshold cycle (ct) values and colony-forming units (cfu) on heym for fresh and thawed ...201020807920
environmental contamination with mycobacterium avium subsp. paratuberculosis in endemically infected dairy herds.environmental contamination with mycobacterium avium subsp. paratuberculosis (map) is thought to be one of the primary sources of infection for dairy cattle. the exact link between fecal shedding of map by individual cows and environmental contamination levels at the herd level was explored with a cross-sectional analysis of longitudinally collected samples on 3 dairy farms. composite samples from multiple environmental sites in 3 commercial dairy herds in the northeast us were cultured quarterl ...201121775002
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