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analysis of infectious laryngotracheitis virus isolates from ontario and new brunswick by the polymerase chain reaction.the polymerase chain reaction (pcr) was used to amplify dna of infectious laryngotracheitis virus (iltv) isolates obtained from field specimens. the examined 47 samples included 37 isolates representing 35 cases of infectious laryngotracheitis from ontario and 10 isolates originating from 10 field cases in new brunswick. the viruses were grown in either embryonated chicken eggs or cell culture, the dna extracted and amplified using primers designed from the sequence information of a 1.1 kb bamhi ...19989442943
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