| demonstration of virus in groundwater after effluent discharge onto soil. | the survival of virus present in secondary effluents discharged into a cypress dome was studied. isolations were made from concentrates of water drawn from 10-foot (304.80 cm) deep wells. data presented show vertical and lateral virus movement as well as survival within the dome for 28 days during a period of heavy rains when no effluent was being applied. due to the inefficiency of virus concentration procedures, it is proposed that much of the virus present was probably not demonstrated. a rap ... | 1975 | 168809 |
| virus detection in soils: a comparison of four recovery methods. | among nine eluents tested, 0.5% (w/v) isoelectric casein at ph 9.0 and 0.5% (w/v) non-fat dry milk (ph 9.0) were the most efficient in eluting poliovirus type 1 (sabin) from eustis fine sand. however, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fat dry milk, isoelectric casein, beef extract, and glycine-edta methods. high overall recovery (75%) of low input (200 pfu) of viruses from 100 g of soil was achieved by the isoelect ... | 1979 | 230890 |
| encephalomyocarditis virus infections in florida, 1960-1966. | | 1967 | 4293832 |
| an "outbreak" of juvenile diabetes mellitus: consideration of a viral etiology. | | 1974 | 4371445 |
| meningococcal and echo-9 meningitis. report of an outbreak. | | 1970 | 4983417 |
| enterovirus-associated illness--florida, arizona, tennessee, 1981. | | 1981 | 6267442 |
| isolation of enterovirus 70 from patient with acute hemorrhagic conjunctivitis-- key west, florida. | | 1981 | 6270522 |
| isolation of enterovirus 70 from patients with acute hemorrhagic conjunctivitis in key west, florida. | | 1981 | 6273726 |
| epidemic viral keratoconjunctivitis diagnosis and management. | the author reports an epidemic of adeno- and enteroviral eye infections at an overseas military complex in the third quarter of 1981. a similar epidemic was reported by the center for disease control, atlanta, ga to also have occurred in florida, latin america, and north carolina at approximately the same time. the differential diagnosis between epidemic keratoconjunctivitis, pharyngoconjunctival fever, acute hemorrhage conjunctivitis and chlamydial infections is described. epidemiology and mana ... | 1983 | 6302154 |
| oligonucleotide fingerprint analysis of enterovirus 70 isolates from the 1980 to 1981 pandemic of acute hemorrhagic conjunctivitis: evidence for a close genetic relationship among asian and american strains. | enterovirus 70 isolates obtained in asia and the americas between 1980 and 1981 from cases of acute hemorrhagic conjunctivitis were found to be very closely related by rnase t1 oligonucleotide fingerprinting. two closely related isolates from the first acute hemorrhagic conjunctivitis epidemic (1969 to 1972) differed by many oligonucleotides from the 1980 to 1981 pandemic strains. the strong similarities of oligonucleotide patterns of isolates from the same epidemic but from distant regions of t ... | 1983 | 6307877 |
| clinical findings and results of treatment in an outbreak of acute hemorrhagic conjunctivitis in southern florida. | an epidemic of acute hemorrhagic conjunctivitis in miami, florida, involved approximately 800 documented cases and more than 2,500 suspected cases. this epidemic was caused by an enterovirus 70 infection affecting primarily young black people residing within a high-risk area. acute hemorrhagic conjunctivitis is characterized by the rapid onset of swollen eyelids, foreign-body sensation, burning, watery discharge, and, usually, bilateral ocular involvement. signs include distinctive bulbar conjun ... | 1983 | 6849368 |
| detection of viral pathogens by reverse transcriptase pcr and of microbial indicators by standard methods in the canals of the florida keys. | in order to assess the microbial water quality in canal waters throughout the florida keys, a survey was conducted to determine the concentration of microbial fecal indicators and the presence of human pathogenic microorganisms. a total of 19 sites, including 17 canal sites and 2 nearshore water sites, were assayed for total coliforms, fecal coliforms, escherichia coli, clostridium perfringens, enterococci, coliphages, f-specific (f(+)) rna coliphages, giardia lamblia, cryptosporidium parvum, an ... | 1999 | 10473424 |
| detection, quantitation and identification of enteroviruses from surface waters and sponge tissue from the florida keys using real-time rt-pcr. | a method was developed for the quantitative detection of pathogenic human enteroviruses from surface waters in the florida keys using taqman (r) one-step reverse transcription (rt)-pcr with the model 7700 abi prism (r) sequence detection system. viruses were directly extracted from unconcentrated grab samples of seawater, from seawater concentrated by vortex flow filtration using a 100 kd filter and from sponge tissue. total rna was extracted from the samples, purified and concentrated using spi ... | 2002 | 12153016 |
| a continuing surveillance of enterovirus infections in healthy children in six united states cities. i. viruses isolated during 1960 and 1961. | | 1963 | 14071359 |
| presence of pathogens and indicator microbes at a non-point source subtropical recreational marine beach. | swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. this study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions at a subtropical, recreational marine beach in south florida impacted by non-point sources of pollution. twelve water and eight sand samples were collected during four sampling eve ... | 2010 | 19966020 |
| preliminary evidence for human fecal contamination in corals of the florida keys, usa. | corals and reef environments are under increased stress from anthropogenic activities, particularly those in the vicinity of heavily populated areas such as the florida keys. the potential adverse impacts of wastewater can affect both the environment and human health; however, because of the high decay rate of bacterial indicators in coral reef waters it has been difficult to document the presence of microbial contaminants and to assign risks in these environments. here we show initial evidence ... | 2002 | 12222890 |
| assessment and impact of microbial fecal pollution and human enteric pathogens in a coastal community. | the goals of this study were to assess watersheds impacted by high densities of osds (onsite sewage disposal systems) for evidence of fecal contamination and evaluate the occurrence of human pathogens in coastal waters off west florida. eleven stations (representing six watersheds) were intensively sampled for microbial indicators of fecal pollution (fecal coliform bacteria, enterococci, clostridium perfringens and coliphage) and the human enteric pathogens, cryptosporidium, giardia, and enterov ... | 2001 | 11381749 |
| acute hemorrhagic conjunctivitis investigation of a large-scale community outbreak in dade county, florida. | an outbreak of acute hemorrhagic conjunctivitis (ahc) due to enterovirus type 70 occurred in dade county, florida, from september to december 1981. younger age groups, members of larger households, and the poor were at significantly greater risk of acquiring ahc than others in the community. schoolchildren were most likely to introduce ahc into the household. after exclusion of all affected children from school, there was a dramatic reduction in reported cases, with attack ratesups, members of l ... | 1983 | 6827704 |
| virus transport and survival after land application of sewage sludge. | the survival and transport patterns of poliovirus 1 and echovirus 1 were studied in undisturbed soil cores which were treated with digested sludge and exposed to natural weather conditions prevailing in north central florida. it was shown that, under those experimental conditions, enteroviruses are relatively rapidly inactivated in the soil. a more rapid virus decline was observed during the warm and dry fall season than during the warm and wet summer season. the monitoring of soil core leachate ... | 1984 | 6331308 |
| neisseria gonorrhoeae conjunctivitis. an outbreak during an epidemic of acute hemorrhagic conjunctivitis. | ten patients with gonococcal conjunctivitis were examined during an epidemic of acute hemorrhagic conjunctivitis (ahc). eye cultures in all cases demonstrated neisseria gonorrhoeae, and seven also had isolates of n gonorrhoeae in genital specimens. all patients responded well to antimicrobial therapy. the patients had used a folk remedy in which they had applied urine to their eyes to treat the symptoms of ahc. | 1983 | 6308287 |
| acute hemorrhagic conjunctivitis, key west, florida. an assessment of risk factors for introduction of illness into households and secondary spread during the 1981 epidemic. | from august 22 to november 6, 1981, 989 cases of acute hemorrhagic conjunctivitis were identified in key west, florida. the outbreak probably began when an infected 12-year-old key west resident returned from the bahamas and transmitted the illness to several neighborhood playmates. the rate of illness was highest for school-aged children, and a high proportion of cases occurred among blacks living in two adjacent low-income housing tracts. a telephone survey of persons living in these two tract ... | 1984 | 6093501 |
| detection of nucleic acids homologous to human immunodeficiency virus in wastewater. | raw wastewaters were obtained from the cities of belle glade, ocala and gainesville in the state of florida and were concentrated using several established methods for the recovery of human enteroviruses. the nucleic acids were then extracted from the wastewater concentrates, suspended in 2 x ssc with and without 2 n naoh (for the detection of dna and both dna and rna, respectively), and dot blotted onto hybridization membranes. these membranes were then hybridized with three 32p-end-labeled 18- ... | 1991 | 1783678 |
| evaluation of sewage source and fate on southeast florida coastal reefs. | water, sponge and coral samples were collected from stations impacted by a variety of pollution sources and screened for human enteric viruses as conservative markers for human sewage. while human enteroviruses and adenoviruses were not detected, noroviruses (nov; human genogroups i and ii) were detected in 31% of samples (especially in sponge tissue). stations near inlets were the only ones to show multiple sample types positive for nov. fecal indicator bacteria and enteric viruses were further ... | 2011 | 21962922 |
| Daily measures of microbes and human health at a non-point source marine beach. | Studies evaluating the relationship between microbes and human health at non-point source beaches are necessary for establishing criteria which would protect public health while minimizing economic burdens. The objective of this study was to evaluate water quality and daily cumulative health effects (gastrointestinal, skin, and respiratory illnesses) for bathers at a non-point source subtropical marine recreational beach in order to better understand the inter-relationships between these factors ... | 2011 | 21976192 |
| human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the upper florida keys. | to address the issue of human sewage reaching corals along the main reef of the florida keys, samples were collected from surface water, groundwater and coral [surface mucopolysaccharide layers (sml)] along a 10 km transect near key largo, fl. samples were collected semi-annually between july 2003 and september 2005 and processed for faecal indicator bacteria (faecal coliform bacteria, enterococci and clostridium perfringens) and human-specific enteric viruses (enterovirus rna and adenovirus dna ... | 2010 | 20105219 |
| groundwater quality impacts from the land application of treated municipal wastewater in a large karstic spring basin: chemical and microbiological indicators. | geochemical and microbiological techniques were used to assess water-quality impacts from the land application of treated municipal wastewater in the karstic wakulla springs basin in northern florida. nitrate-n concentrations have increased from about 0.2 to as high as 1.1 mg/l (milligrams per liter) during the past 30 years in wakulla springs, a regional discharge point for groundwater (mean flow about 11.3 m(3)/s) from the upper floridan aquifer (ufa). a major source of nitrate to the ufa is t ... | 2009 | 19232432 |
| analysis of multiple enteric viral targets as sewage markers in coral reefs. | water and coral mucus samples were collected from throughout the florida keys national marine sanctuary and the dry tortugas for three years and were analyzed for human enteric viruses (enteroviruses, noroviruses, hepatitis a virus and adenoviruses) as conservative markers of human sewage using molecular methods. of the 100 coral and water samples collected, 40 contained genetic material from one or more human enteric viruses. dna-based adenoviruses were detected widely, in 37.8% of samples and ... | 2007 | 17881013 |
| microbiological assessment of ambient waters and proposed water sources for restoration of a florida wetland. | this study evaluated the microbial quality of reclaimed and storm water as proposed sources for restoration of a florida wetland. bacterial indicators, bacteriophages and waterborne pathogenic microorganisms (cryptosporidium, giardia and infectious enteric viruses) were analysed during a 1-year period in order to determine potential public health risks associated with exposure to the proposed water sources for restoration. ambient waters within the wetland (four active water wells and four major ... | 2005 | 16075936 |
| presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the florida keys. | concerns about the presence of enteric viruses in the surface waters of the florida keys prompted analyses of virus stability and persistence in these waters. in an in vitro study we evaluated the survival of poliovirus and stability of viral rna in filtered natural seawater (fsw), unfiltered natural seawater (usw), artificial seawater (asw) and di water. this study compared cell culture infectivity with direct reverse transcription-polymerase chain reaction analysis. attenuated poliovirus was s ... | 2004 | 15041426 |