| a novel rt-multiplex pcr for enteroviruses, hepatitis a and e viruses and influenza a virus among infants and children with diarrhea in vietnam. | a novel reverse transcription-multiplex polymerase chain reaction (rt-multiplex pcr) assay that can detect enteroviruses, hepatitis a and e viruses and influenza a virus from various hosts (avian species, human, swine and horse) was developed. the identification of that group of viruses was performed with the mixture of four pairs of published specific primers (f1 and r1, p3 and p4, 2s and 2as, mmu42 and mmu43) for amplifying viral genomes and specifically generated four different amplicon sizes ... | 2005 | 15645373 |
| epidemiologic and virologic investigation of hand, foot, and mouth disease, southern vietnam, 2005. | during 2005, 764 children were brought to a large children's hospital in ho chi minh city, vietnam, with a diagnosis of hand, foot, and mouth disease. all enrolled children had specimens (vesicle fluid, stool, throat swab) collected for enterovirus isolation by cell culture. an enterovirus was isolated from 411 (53.8%) of the specimens: 173 (42.1%) isolates were identified as human enterovirus 71 (hev71) and 214 (52.1%) as coxsackievirus a16. of the identified hev71 infections, 51 (29.5%) were c ... | 2007 | 18217559 |
| poliomyelitis-like illness due to japanese encephalitis virus. | acute flaccid paralysis remains common among vietnamese children despite a pronounced fall in the incidence of poliomyelitis. | 1998 | 9660579 |
| the emergence of rotavirus g12 and the prevalence of enteric viruses in hospitalized pediatric diarrheal patients in southern vietnam. | diarrhea is a major cause of childhood morbidity and mortality in developing countries, and the majority of infections are of viral etiology. we aimed to compare the etiological prevalence of the major enteric viruses in an urban and a rural setting in southern vietnam. we simultaneously screened fecal specimens from 362 children in ho chi minh city and dong thap province that were hospitalized with acute diarrhea over a 1-month-long period for four viral gastrointestinal pathogens. rotavirus wa ... | 2011 | 21976585 |
| viral etiology of encephalitis in children in southern vietnam: results of a one-year prospective descriptive study. | acute encephalitis is an important and severe disease in children in vietnam. however, little is known about the etiology while such knowledge is essential for optimal prevention and treatment. to identify viral causes of encephalitis, in 2004 we conducted a one-year descriptive study at children's hospital number one, a referral hospital for children in southern vietnam including ho chi minh city. | 2010 | 21049060 |
| development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus a16. | human enterovirus 71 (hev71) and coxsackievirus a16 (cva16) are two major aetiological agents of hand, foot and mouth disease (hfmd) in children. recently there have been several large outbreaks of hfmd in vietnam and the asia-pacific region. in this study, a multiplex rt-pcr assay was developed in order to detect simultaneously hev71, cva16 and other human enteroviruses. enterovirus detection was performed with a mixture of three pairs of oligonucleotide primers: one pair of published primers f ... | 2010 | 20863857 |
| identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in ho chi minh city, vietnam. | a total of 276 fecal specimens collected from infants and children admitted to hospital with acute gastroenteritis in ho chi minh city, vietnam from october 2002 to september 2003, were tested for the presence of enteroviruses by rt-pcr and virus isolation. enteroviruses were detected in 27 patients by rt-pcr corresponding to 9.8%. however, only four enterovirus strains could be isolated by cell culture with two different cell lines caco2 and vero, showing specific cytopathic effect (cpe). the r ... | 2005 | 16121381 |
| viral etiologies of acute respiratory infections among hospitalized vietnamese children in ho chi minh city, 2004-2008. | the dominant viral etiologies responsible for acute respiratory infections (aris) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by aris are highest. improved etiological insight is needed to improve clinical management and prevention. | 2011 | 21455313 |
| the seroprevalence and seroincidence of enterovirus71 infection in infants and children in ho chi minh city, viet nam. | enterovirus 71 (ev71)-associated hand, foot and mouth disease has emerged as a serious public health problem in south east asia over the last decade. to better understand the prevalence of ev71 infection, we determined ev71 seroprevalence and seroincidence amongst healthy infants and children in ho chi minh city, viet nam. in a cohort of 200 newborns, 55% of cord blood samples contained ev71 neutralizing antibodies and these decayed to undetectable levels by 6 months of age in 98% of infants. th ... | 2011 | 21765891 |