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immunoelectrophoretic study on common antigens of são lourenço da mata and belo horizonte strains of schistosoma mansoni adult worms and biomphalaria snails.immunoelectrophoretic studies on common antigens were carried out by using rabbits sera immunized against são lourenço da mata and belo horizonte strains of schistosoma mansoni adult worms and antigens of biomphalaria glabrata pigmented (jaboatão--pe); b. glabrata albino (belo horizonte--mg) and b. straminea (são lourenço da mata, pe). furthermore, the reverse approach was proceeded, namely, sera anti biomphalaria snails produced in rabbits were tested against both strains of schistosoma adult w ...19921284896
the threat of reintroduction of natural transmission of chagas' disease in bambuí, minas gerais state, brazil, due to panstrongylus megistus.in the epidemiological surveillance program in the county of bambuí, minas gerais, between august 1986 and december 1988, 154 panstrongylus megistus were captured by the local population in both peridomicile and intradomicile environments. fifteen (9.8%) of the p. megistus harboured trypanosoma cruzi. precipitin tests showed that the most frequent triatomine blood meal sources were birds, but other sources were dogs, men and cats. the isoenzyme characterization of 13 t. cruzi strains showed that ...19921308572
studies on common antigenicities between the belo horizonte strain, brazil of schistosoma mansoni eggs and biomphalaria snails by immunoelectrophoresis.immunoelectrophoretic studies of common antigenicities were carried out by using rabbit sera immunized against the belo horizonte strain of schistosoma mansoni eggs and crude antigens of biomphalaria snails and vice versa. with regard to common antigenicities between s. mansoni eggs and biomphalaria snails, s. mansoni eggs produced 4 to 5 bands with biomphalaria glabrata pigmentado, 3 to 4 bands with b. glabrata albino and only 1 band with b. straminea. in our laboratory, the infection rate of s ...19921381846
cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil.anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ...200717278086
rickettsial infections of dogs, horses and ticks in juiz de fora, southeastern brazil, and isolation of rickettsia rickettsii from rhipicephalus sanguineus ticks.the present study was performed in an area endemic for brazilian spotted fever (bsf) in juiz de fora, state of minas gerais, brazil, during the years 2007 and 2008, when fatal cases of bsf (caused by rickettsia rickettsii) were reported. adult ticks (acari: ixodidae) identified as rhipicephalus sanguineus (latreille) and amblyomma cajennense (fabricius) were collected from dogs and horses, respectively, and tested by polymerase chain reaction (pcr). overall, 13.1% of the rh. sanguineus ticks and ...201020950356
aspects of the maintenance of the life cycle of fasciola hepatica in lymnaea columella in minas gerais, brazil.fascioliasis is a parasitic disease of domestic ruminants that occurs worldwide. the lymnaeid intermediate hosts of fasciola hepatica include lymnaea columella, which is widely distributed in brazil. a colony of l. columella from belo horizonte, mg, was reared in our laboratory to be used in studies of the f. hepatica life cycle, the intermediate host-parasite relationship and development of an anti-helminthic vaccine. in the first experiment 1,180 snails were exposed to miracidia of f. hepatica ...200212048573
intraspecific variation in the venoms of the south american rattlesnake (crotalus durissus terrificus).the venom of eight individual crotalus durissus terrificus snakes from the state of minas gerais, brazil, in addition to pooled venom from butantan institute, were compared. snakes were captured in distinct locations, some of them 600 km apart: conselheiro lafaiete, entre rios de minas, itauna, itapecerica, lavras, patos de minas, paracatu, and santo antonio do amparo. the crude venoms were tested for proteolytic, phospholipase a2, platelet aggregating, and hemagglutinating activities. the venom ...200011081410
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