| ionophore antibiotic (narasin) poisoning in rabbits. | outbreaks of narasin poisoning in rabbits from several commercial rabbit-raising farms in the state of parana, brazil, are reported. approximately 5,000/35,000 rabbits died after having consumed a pelleted ration to which poultry ration premix had been added. clinical signs included apathy, anorexia, muscle weakness, impaired walking, diarrhea, respiratory distress, and opistothonus. gross findings were not remarkable, but varying degrees of degeneration, necrosis and regeneration of skeletal mu ... | 1994 | 7839570 |
| leishmania braziliensis: isolation of carbohydrate-containing antigen and possibility of its use in the immunodiagnosis of american cutaneous leishmaniasis. | leishmania braziliensis is a causative agent of american cutaneous leishmaniasis (acl). the 034-jcg strain, isolated from a patient from the northern region of paraná state, brazil, was cultivated in blood agar base medium, lyophilized and submitted to phenol-water extraction. the extract was treated with rnase i. the carbohydrate containing-antigen (ag-cho) was immunogenic to rabbits and showed at least a fraction with some negative charge at ph 8.2. this antigen showed cross-reactivity with th ... | 1995 | 8525271 |
| bovine herpesvirus type 1 abortions detected by a semi-nested pcr in brazilian cattle herds. | bovine herpesvirus type 1 (bhv-1) was investigated by a semi-nested polymerase chain reaction (sn-pcr) and by mdbk cell culture virus isolation in organ fragments from 55 aborted fetuses collected from beef and dairy cattle herds with history of reproductive problems in the north of paraná state, brazil. a 425 bp amplicon of the bhv-1 glycoprotein d gene was detected in 14 (25.4%) aborted fetuses. bhv-1 was isolated in mdbk cells from the tissue of 5 (9.1%) fetuses. the specificity of positive r ... | 2004 | 15894030 |
| cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil. | anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ... | 2007 | 17278086 |
| [preliminary study on feeding preference of nyssomyia neivai (pinto) and nyssomyia whitmani (antunes & coutinho) (diptera: psychodidae) in a rural area of the state of paraná, south brazil]. | due to the importance of sandflies in the american cutaneous leishmaniasis epidemiology, we investigated the feeding preference of sandflies related to domestic animals common in domiciliary environments in endemic rural areas, where this disease occurs. the sandflies were collected with falcão light traps, installed in cages, each one containing one domestic animal kind (pig, dog, rabbit or chicken), in recanto marista, in doutor camargo municipality. forty five collections were made, totaling ... | 2007 | 18246272 |