enzyme-linked immunosorbent assays for detecting antibody to rickettsia australis in sera of various animal species. | new endemic areas of spotted fever-like rickettsial disease have been found in south-eastern australia (gippsland, victoria and flinders island, tasmania). the rickettsia responsible is currently unknown although it may be rickettsia australis. to investigate serological evidence of rickettsial exposure in various wild animal species, a competitive elisa was developed which detected antibodies to r. australis. it was based on inhibition of an indirect elisa detecting antibody to r. australis in ... | 1990 | 2292182 |
geographically diverse australian isolates of melissococcus pluton exhibit minimal genotypic diversity by restriction endonuclease analysis. | melissococcus pluton, the causative agent of european foulbrood is an economically significant disease of honey bees (apis mellifera) across most regions of the world and is prevalent throughout most states of australia. 49 isolates of m. pluton recovered from diseased colonies or honey samples in new south wales, queensland, south australia, tasmania and victoria were compared using sds-page, western immunoblotting and restriction endonuclease analyses. dna profiles of all 49 geographically div ... | 1999 | 10227161 |
first isolation of an aquatic birnavirus from farmed and wild fish species in australia. | during routine sampling and testing, as part of a systematic surveillance program (the tasmanian salmonid health surveillance program), an aquatic birnavirus was isolated from 'pin-head' (fish exhibiting deficient acclimatisation on transfer to saltwater) atlantic salmon salmo salar, approximately 18 mo old, farmed in net-pens located in macquarie harbour on the west coast of tasmania, australia. the isolate grows readily in a range of fish cell lines including chse-214, rtg-2 and bf-2 and is ne ... | 2000 | 11129376 |
serological responses against the pathogenic dimorphic fungus mucor amphibiorum in populations of platypus (ornithorhynchus anatinus) with and without ulcerative mycotic dermatitis. | mucor amphibiorum, a dimorphic fungus, causes ulcerative dermatitis and systemic infections in the platypus ornithorhynchus anatinus in some river systems in tasmania but apparently not in other regions of australia. as yet there are no suitable tests for population surveys, nor for detection of internal lesions in live animals. consequently, immunoglobulins were purified from the serum of platypuses and anti-immunoglobulin antisera were prepared in rabbits in order to develop an enzyme-linked i ... | 2002 | 12079747 |
cultured gill-derived neoparamoeba pemaquidensis fails to elicit amoebic gill disease (agd) in atlantic salmon salmo salar. | amoebic gill disease (agd) affects the culture of atlantic salmon salmo salar in the southeast of tasmania. the disease is characterised by the presence of epizoic neoparamoeba spp. in association with hyperplastic gill tissue. gill-associated amoebae trophozoites were positively selected by plastic adherence for culture in seawater, where they proliferated using heat-killed e. coli as a nutrient source. one isolate of gill-harvested amoebae designated np251002 was morphologically consistent to ... | 2005 | 16231639 |