| detection of spotted fever group rickettsiae in ticks and rodents by polymerase chain reaction technique in people's republic of china. | the polymerase chain reaction (pcr) technique for amplification of genomic fragments of spotted fever group (sfg) rickettsiae directly from field samples of ticks, tick ova, tick larvae, tick faeces and organs of wild mice was employed for the first time in p.r. of china. ticks and rodents were collected in beijing and heilongjiang, hainan and hebei provinces. the pcr primers were designed from the dna sequence encoding the 190 k protein of r. rickettsii for a 532 bp long product. seven of ten t ... | 1995 | 8722295 |
| [seroprevalence of antibody against human metapneumovirus in beijing]. | to understand the seroprevalence of antibody against the newly identified human metapneumovirus (hmpv) in beijing. | 2005 | 16412351 |
| the w-beijing lineage of mycobacterium tuberculosis overproduces triglycerides and has the dosr dormancy regulon constitutively upregulated. | the beijing family of mycobacterium tuberculosis strains has been associated with epidemic spread and an increased likelihood of developing drug resistance. the characteristics that predispose this family to such clinical outcomes have not been identified, although one potential candidate, the phenolic glycolipid pgl-tb, has been shown to mediate a fulminant lethal disease in mice and rabbits due to lipid-mediated immunosuppression. however, pgl-tb is not uniformly expressed throughout the beiji ... | 2007 | 17237171 |
| [prokaryotic expression and antigenic activity analysis on the matrix protein genes of two strains of human metapneumovirus recently identified in beijing]. | human metapneumovirus (hmpv) is a recently identified respiratory virus more like human respiratory syncytial virus in clinical symptoms. matrix protein (m) is one of the most important structural proteins. for further studying of hmpv, the full length of m genes from the recombinant plasmid pucm-m1816 and pucmm1817 were cloned by pcr and sub-cloned into the pet30a(+) vector, which is a prokaryotic expression vector, after dual-enzyme digestion with bam hi and xho i. the positive recombinated pl ... | 2007 | 17886723 |
| [seroprevalence of antibody against human bocavirus in beijing, china]. | to find out the importance of human bocavirus (hbov) as an infectious agent for population in beijing, china, seroprevalence study was conducted by using expressed recombinant major capsid vp2 protein as an antigen. | 2008 | 19099684 |
| study on prevalence and genotype of hepatitis e virus isolated from rex rabbits in beijing, china. | summary. a novel genotype of hepatitis e virus (hev) isolated from rabbits is reported. the aim of this study was to confirm and further investigate the prevalence of the novel hev genotype in rabbits in china. sera and faecal samples were collected from farmed rex rabbits in beijing, china. all serum samples were tested for anti-hev antibody by eia. both the serum and the faecal samples were evaluated for detection of hev rna using a nested rt-pcr assay. the nucleotide sequences of rabbit hev w ... | 2010 | 20609076 |
| demo-experiments of bonghan ducts in beijing. | | 2009 | 20648726 |
| [sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china]. | to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application. | 2010 | 20818074 |