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[studies on the strain differences of schistosoma japonicum in the mainland of china. xi. enzyme linked immunoelectrotransfer blot analysis].antigens prepared from s. japonicum adult worms of different isolates i. e. anhui, hubei, guangxi, yunnan and sichuan by origin were analyzed by enzyme linked immunoelectrotransfer blot (eitb) probed with rabbit anti-snail antibody (figs 1,2). anti-sera against oncomelania h. hupensis from anhui, hubei, yunnan and sichuan localities were prepared separately and used in the tests. the eitb patterns were similar in s. j. isolates of anhui and hubei, and it was also the case among s. j. isolates of ...19921303328
characteristics of different isolates of schistosoma japonicum from china in the final hosts.the present paper deals with the susceptibility of common laboratory animals, such as mouse, rat, hamster, jird, rabbit and rhesus monkey, to infection with different isolates of schistosoma japonicum in the mainland of china under laboratory conditions. with the exception of the rat, all the animals under study were permissive hosts for different isolates though their worm recovery rates varied. the mean body length of pair-worms of the yunnan isolate was considerably smaller than that of the a ...19911948285
[studies on the strain differences of schistosoma japonicum in the mainland of china. iv. fuzzy cluster analysis on some biological characters of different isolates of schistosoma japonicum].in this paper, a computerized numerical systematics was used to analyse the 5 different isolates of schistosoma japonicum in the mainland of china by means of fuzzy clustering method with 32 quantitative data of each isolate about the morphological and biological characters. a dendrogram of the 5 isolates of s. japonicum was constructed on the basis of similarity coefficients as shown. the phylogenetic relationships revealed by the present study show that the two isolates of anhui and hubei get ...19911959166
[studies on the strain differences of schistosoma japonicum in the mainland of china. ii. susceptibility of mammalian hosts].six species of animals were percutaneously exposed to cercariae obtained from pools of naturally infected snails from different isolates of s. japonicum in the mainland of china as shown in tables 1 and 2. our results indicated that with the exception of rat, all animals under study were permissive host though their worm recovery rates varied with different isolates. the mean prepatent period in different host species were 35.0 +/- 0.8 to 36.4 +/- 1.0 days for anhui isolate; 34.5 +/- 1.2 to 36.4 ...19902099256
[main reservoirs of infection of the hemorrhagic fever renal syndrome in endemic areas of anhui province]. 19852862997
sds-page protein pattern and its antigenicity analysis of different isolates of schistosoma japonicum in china.homogenates prepared from s. japonicum adult worms of different isolates from anhui, hubei, guangxi, yunnan and sichuan provinces were analyzed by sds-page and enzyme linked immunoelectrotransfer blot (eitb) tested with rabbit anti-snails antibody. the results of sds-page indicated that with silver staining both male and female worms of guangxi isolate showed some definite differences in their protein profile, namely, absence of one band between 50-75 kda in male worms and marked reduction in qu ...19948187569
variation in the sequence of a mitochondrial nadh dehydrogenase i gene fragment among six natural populations of schistosoma japonicum from china.the present study investigated the level of genetic variation among schistosoma japonicum populations of different geographical origins from mainland china. polymerase chain reaction-based methods were employed to determine the sequence for a subunit of the mitochondrial nadh dehydrogenase i gene for populations from zhejiang, anhui, jiangxi, hunan, hubei and sichuan. no variation was detectable in the nadh dehydrogenase i sequence within populations from zhejiang and hubei, whereas sequence var ...19989925274
an electrophoretic comparison of schistosoma japonicum (trematoda) from different provinces in the people's republic of china suggests the existence of cryptic species.schistosoma japonicum from the people's republic of china is considered to represent a single species comprising either 1 or 4 'strains'. we conducted an allozyme electrophoretic study to examine the extent of genetic variation in s. japonicum from mainland china. the allelic profiles of s. japonicum from 7 provinces were established at 16 enzyme loci. s. japonicum from sichuan had 3-5 (19-31%) fixed differences compared with those from zhejiang, anhui, jiangxi, hunan, hubei and yunnan, suggesti ...199910581616
issr, an effective molecular approach for studying genetic variability among schistosoma japonicum isolates from different provinces in mainland china.in the present study, inter-simple sequence repeats (issrs) markers were used to examine the genetic variability of schistosoma japonicum isolates from different provinces in mainland china, using s. japonicum from japan and s. mansoni from puerto rico for comparison. of the 30 primers screened, 4 produced highly reproducible issr fragments. using these primers, 107 discernible dna fragments were generated with 105 (98.13%) being polymorphic, indicating considerable genetic variation among the e ...200919539784
[sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china].to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application.201020818074
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