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[epidemiological study on toxoplasma infection in human beings and animals in shandong province].a total of number of 6822 samples of human serum and 3093 samples of animal serum collected from 7 prefectures and cities were detected for the anti-toxoplasma antibodies by using the indirect hemagglutination (iha) test in shandong province. the positive rate of population was 4.06 +/- 0.24%. the antibody positive rates of swine, sheep, rabbits, chicken and ducks were 14.05%, 8.52%, 2.43%, 2.98% and 23.33% respectively. after the data were treated with groups analysis and stratified analysis, i ...19892736611
[the investigation of the first outbreak of tularemia in shandong peninsula]. 19873449210
isolation and characterization of clostridium perfringens from apparently healthy animals of the shandong province of china.in a pilot study the presence and frequency of clostridium (c.) perfringens was investigated among apparently healthy farm animals in the shandong province of china. 748 faecal samples were collected from 9 pig-, 4 sheep-, 7 cattle- and 5 rabbit farms. c. perfringens was isolated from 124 samples (16.6%). the isolates were classified into major toxin types by using pcr analysis detecting the genes encoding these toxins. all isolates were identified as c perfringens toxin type a. there are also s ...200717970339
[sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china].to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application.201020818074
prokaryotic expression and potential application of the truncated pcv-2 capsid protein.three pairs of specific primers were designed to amplify the f2-1, f2-2 and xf2-2 truncated sequences of orf2 which encodes the capsid protein of porcine circovirus type 2 (pcv-2). the f2-1 sequence had most of the nls region of orf2, but the f2-2 and xf2-2 genes had the nls region deleted. truncated genes were subcloned into pet-32a(+) vectors to construct recombinant fusion expression vectors. the vectors were then transformed into rosetta(de3) e. coli and expressed by induction of iptg. expre ...201020960305
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