| host preference of mosquito vectors of japanese encephalitis. | the host preference of 4 culex mosquito species collected in miaoli and pingtung counties, taiwan was studied by capillary precipitin method. antisera to alum-precipitated sera of man, bovine, swine, rabbit, horse, dog, cat, mouse, chicken, duck, and pigeon were produced in rabbits and reacted with 758 mosquito blood meals among which reactions to one or more antisera. culex annulus and culex tritaeniorhynchus summorosus showed a great avidity for pig, and culex fuscocephala for bovine. culex pi ... | 1975 | 181218 |
| reovirus antibodies among animals in taiwan. | serum specimens for reovirus antibody survey were collected from 60 pigs, 50 buffalos, 30 rabbits, 30 guinea pigs, 20 albino rats, 60 swiss mice, 15 taiwan monkeys, 14 goats and 12 dogs. serum antibodies were measured by the hemagglutination inhibition (hi) test. hi antibodies to reovirus were found in a high percentage of pigs, albino rats, swiss mice, dogs and goats, and less frequently in rabbits, guinea pigs, taiwan monkeys and buffalos. | 1976 | 827427 |
| a review of the biology of the chinese and formosan strains of schistoma japonicum. | | 1976 | 1025722 |
| [preparation of toxoid from taiwan cobra (naja naja atra) venom]. | potency of sixty antitoxic unit was reached after two immunizations in 2-week intervals of rabbits and horses with 10-25 mg taiwan cobra (naja naja atra) venom which was detoxified by 0.125% glutaraldehyde. now this procedure has become a routine antivenine-producing method by which snake bivalent neurotropic antivenine is produced. the stability test showed that taiwan cobra toxoid kept at 37 degrees c for 40 days, the antigenicity increased by 24% and toxicity decreased by 10% as compared to t ... | 1990 | 2176918 |
| differentiation of poliovirus type i isolates in the 1982 epidemic from sabin-i strain by antigenic and temperature markers. | poliovirus type 1 strains recovered from patients with poliomyelitis during the epidemic of 1982, were distinct antigenically from sabin-1 strain by comparing homologous and heterologous neutralization rate constants of sabin-1 specific antiserum. with the reproductive capacity at 40 degrees c, these isolates could be easily differentiated from vaccine strain. it was suggested that the epidemic of poliomyelitis in 1982 was not caused by vaccine strain. the 24 isolates reacted with lsc and/or sab ... | 1987 | 3036431 |
| correlation of placental microsomal activities with protein detected by antibodies to rabbit cytochrome p-450 isozyme 6 in preparations from humans exposed to polychlorinated biphenyls, quaterphenyls, and dibenzofurans. | placental tissues were obtained from chinese women in taiwan who had been exposed to contaminated rice oils containing polychlorinated biphenyls and their thermal degradative products. exposure via the diet occurred 4-5 years prior to pregnancy. placental microsomal fractions from eight of the nine exposed subjects studied showed marked elevation of benzo(a)pyrene hydroxylation and 7-ethoxyresorufin o-deethylation activities related to control subjects. placental microsomes from exposed subjects ... | 1986 | 3079671 |
| studies on the taiwan folk medicine; iii. a smooth muscle relaxant from onychium siliculosum, onitin. | | 1985 | 4034731 |
| antibodies to epstein-barr virus in a burkitt's lymphoma cell line in taiwan monkeys (macaca cyclopis). | the prevalence of taiwan monkeys with antibodies to epstein-barr virus, as detected by the indirect immunofluorescence technique, was lower than that of healthy persons in taiwan. | 1971 | 4324196 |
| further studies on the immunogenic properties of photooxidized formosan snake venoms, with special reference to a trial preparation of polyvalent antivenin using photooxidized multiple venom antigens--second report. | | 1973 | 4515138 |
| an outbreak of pseudorabies in swine in pingtung. | | 1972 | 4679460 |
| paragonimus and paragonimiasis. | | 1969 | 4935271 |
| comparison of naja naja siamensis and naja naja atra venoms. | | 1968 | 4966517 |
| factors responsible for in vitro transformation of inaba and hikojima from ogawa type el tor vibrio. | | 1966 | 5221282 |
| hepatitis-associated antigen and antibody in taiwan. | | 1971 | 5293623 |
| comparison of the genomes of pseudorabies (aujeszky's disease) virus strains by restriction endonuclease analysis. | the dna of pseudorabies virus (prv) strains from the united states and taiwan and attenuated vaccine strains from romania were compared by restriction endonuclease analysis. electrophoretically separated prv dna fragments of kpni and bamhi digests demonstrated cleavage pattern variations which clearly distinguished the 3 taiwan isolates from all other strains, as well as from each other. one type of variation involved the loss or gain of restriction endonuclease cleavage sites. examples of this ... | 1984 | 6098203 |
| studies on trypanosomes in the taiwan monkey. | fifty-three or 9% of 594 taiwan monkeys, macaca cyclopis, were found infected with a trypanosome species. the parasites were recovered from monkey blood in diaphasic blood-agar culture medium with a locke's solution overlay. trypanosomes were found on only a few thick blood smears and no dividing or multiplying forms were found. some of the organisms had a free-flagellum (4.7 microns), an undulating membrane and both ends of the body were pointed. the total length averaged 41.7 microns; nucleus ... | 1983 | 6673131 |
| production of type ii ribotoxins by aspergillus species and related fungi in taiwan. | a molecular investigation was conducted on the production of type ii ribotoxin of the species aspergillus and related fungi in taiwan. species that carried ribotoxin were confirmed by (1) cross-reactivity to anti-alpha-sarcin serum; (2) southern dot hybridization; (3) pcr amplification of genomic dna with specific primers; and (4) analysis of ribotoxic activity. five new strains, a. clavatus, a. oryzae var. effusus, a. ostianus, a. tamarii, and neosartorya fischeri var. spinosa, were identified ... | 1995 | 7778122 |
| cinnamophilin, a novel thromboxane a2 receptor antagonist, isolated from cinnamomum philippinense. | the pharmacological activity of cinnamophilin ((8r,8's)-4,4'-dihydroxy-3,3'-dimethoxy-7-oxo-8,8'-neolignan), isolated from cinnamomum philippinense, was studied in isolated rat aorta, guinea-pig trachea and rabbit platelets. cinnamophilin was found to be a thromboxane a2 receptor blocking agent in these tissues as revealed by its competitive antagonism of the u-46619 (9,11-dideoxymethanoepoxy-9 alpha,11 alpha-prostaglandin f2 alpha)-induced contraction of rat aorta and guinea-pig trachea and agg ... | 1994 | 8026563 |
| antiplatelet constituents of formosan rubia akane. | a known steroid, in addition to triterpenoids, anthraquinones, naphthalenes and a new anthraquinone glycoside, xanthopurpurin 3-o-beta-d-glucoside, were isolated from the roots of rubia akane grown in taiwan. mollugin, a naphthohydroquinone, showed strong inhibition of arachidonic acid (aa)-induced and collagen-induced platelet aggregation. in contrast, 2-methyl-1,3,6-trihydroxyl-9,10-anthraquinone, xanthopurpurin 3-o-beta-d-glucoside, and xanthopurpurin showed mainly strong inhibition of collag ... | 1994 | 8176404 |
| antiplatelet effects and vasorelaxing action of some constituents of formosan plants. | various xanthones as well as quercetin have been shown to exhibit antiplatelet activity. a series of anthraquinones analogues structurally related to xanthones and a series of quercetin-related compounds were tested for their antiplatelet effects. emodin, frangulin b, kaempferol tetraacetate, quercetin-3-o-galactoside octaacetate, rhamnazin triacetate, and rhamnetin tetraacetate were found to be potent antiplatelet agents, and 1,8-dihydroxy-6-methoxy-3-methylanthraquinone 8-o-rhamnosyl-(1-->2)-g ... | 1993 | 8350094 |
| indolopyridoquinazoline alkaloids with antiplatelet aggregation activity from zanthoxylum integrifoliolum. | bioassay-guided fractionation led to the isolation of three indolopyridoquinazoline alkaloids, 1-hydroxyrutaecarpine, rutaecarpine, and 1-methoxyrutaecarpine as the active principles of antiplatelet aggregation in vitro, from the chloroform-soluble part of the fruit of zanthoxylum integrifoliolum (rutaceae). 1-hydroxyrutaecarpine exhibited antiplatelet activity induced by aa and showed an ic50 value of ca. 1-2 micrograms/ml. | 1996 | 8657756 |
| quality of medical care for noninsulin-dependent diabetic patients in a community teaching hospital in taiwan. | to evaluate the quality of diabetes management provided by a community teaching hospital in taipei county, the medical records of 208 noninsulin-dependent diabetes mellitus (niddm) patients who attended the department of internal medicine, taiwan provincial taipei hospital, from july to december 1994, were selected at random and reviewed. first visit examination results were compared to the practice guidelines established by the diabetes association, taiwan roc. the frequency of biochemical test ... | 1996 | 8688711 |
| novel antiplatelet naphthalene from rhamnus nakaharai. | a new naphthalene derivative, isotorachrysone [1], was isolated from the stem bark of rhamnus nakaharai along with several known compounds. the antiplatelet effects of isotorachrysone [1], isotorachrysone peracetate [2], 6-methoxysorigenin [3], quercetin 3-o-methyl ether [4], and quercetin 3-o-methyl ether peracetate [5] were studied using washed rabbit platelets. of the compounds tested, 1, 2, 4, and 5 showed potent antiplatelet effects on arachidonic acid (aa-) and collagen-induced platelet ag ... | 1995 | 8691213 |
| antiplatelet flavonoids from seeds of psoralea corylifolia. | the meoh extract of the seeds of psoralea corylifolia l. was found to inhibit the aggregation of rabbit platelets induced by arachidonic acid, collagen, and platelet activating factor. bioassay-directed fractionation led to the isolation of three flavonoids, isobavachalcone (1), neobavaiso-flavone (2), and bavachin (3). compounds 1 and 2 inhibited platelet aggregation. | 1996 | 8759164 |
| novel antiplatelet constituents from formosan moraceous plants. | sixteen constituents from formosan moraceous plants were tested for their antiplatelet activities in rabbit platelet suspension and human platelet-rich plasma. cycloartocarpin a, cycloheterophyllin, broussochalcone a, kazinol a, broussoaurone a, and broussoflavonol f showed strong inhibition of arachidonic acid (aa)-induced platelet aggregation. of the compounds tested, broussochalcone a exhibited the most potent inhibition of platelet aggregation induced by aa (ic50 = 6.8 microm). the antiplate ... | 1996 | 8864236 |
| viability of metacercariae of clonorchis sinensis in frozen or salted freshwater fish. | metacercariae of clonorchis sinensis were collected from freshwater fish (pseudorasbora parva) after storage in a freezer at -12 degrees c for 10-20 days, in a freezer at -20 degrees for 3-7 days, or treating with heavy salt (fish/salt = 10 gm/3 gm) at 26 degrees for 5-15 days. the metacercariae from the frozen fish were inoculated into 30 rats and eight rabbits, and those from the salted fish into 33 rats. metacercariae from freshwater fish stored at -12 degrees for 10-18 days or -20 degrees fo ... | 1998 | 9602382 |
| characterization of rabbit hemorrhagic disease virus field isolates in taiwan. | liver tissues from animals that were suspected to have died of rabbit hemorrhagic disease (rhd) were used for isolation and characterization of the causative agent. three strains of rhd virus were isolated as the supernatants of liver homogenates reacted positively by hemagglutination (ha) assays and were infective for rabbits after second passage in animals. following extraction of liver homogenates from animals infected with each of three isolates, each virus strain was purified by cscl densit ... | 1998 | 9628218 |
| chemical constituents from cassytha filiformis ii. | using a bioassay-directed fractionation method, three new compounds, including an aporphine alkaloid, cassyformine (4); an oxoaporphine alkaloid, filiformine (8), and a lignan, (+)-diasyringaresinol (10), along with 14 known compounds, were further isolated and characterized from the meoh extract of the fresh herbs of cassytha filiformis. among the isolates of this plant, cathafiline (1), cathaformine (2), actinodaphnine (3), n-methylactinodaphnine (5), predicentrine (6), and ocoteine (7) exhibi ... | 1998 | 9677264 |
| chemical constituents and biological activities of the fruit of zanthoxylum integrifoliolum. | through continuing studies on the chemical constituents and antiplatelet aggregation principles of the fruit of the formosan zanthoxylum integrifoliolum, four new compounds-including two new lignans, (+)-pinoresinol-di-3,3-dimethylallyl ether (1), and (+)-pinoresinol-3,3-dimethylallyl ether (2); zanthonitrile (3), and one new flavonoid, 3,5-diacetyltambulin (4)-and 18 known compounds were isolated from the chcl3-soluble fraction. their structures were elucidated on the basis of spectral data and ... | 1999 | 10395498 |
| aristolactams and dioxoaporphines from fissistigma balansae and fissistigma oldhamii. | investigation of extracts of fissistigma balansae and fissistigma oldhamii resulted in the isolation of 11 aristolactams-stigmalactam (1), piperolactam a (2), piperolactam c (3), aristolactam aii (4), aristolactam aiiia (5), aristolactam bii (6), aristolactam biii (7), aristolactam fii (8), goniothalactam (9), enterocarpam i (10), and velutinam (11)-as well as two dioxoaporphines, noraristolodione (12) and norcepharadione b (13). the new compound 1 was identified by spectral data interpretation. ... | 2000 | 10978218 |
| differential expression and geographic variation of the venom phospholipases a2 of calloselasma rhodostoma and trimeresurus mucrosquamatus. | to investigate the geographic variations in venoms of two medically important pitvipers, we have purified and characterized the phospholipases a2 (pla2s) from the pooled venoms of calloselasma rhodostoma from malaysia, thailand, indonesia, and vietnam, as well as the individual venom of trimeresurus mucrosquamatus collected from both north and south taiwan. enzymatic and pharmacological activities of the purified pla2s were also investigated. the complete amino acid sequences of the purified pla ... | 2001 | 11370849 |
| quinoline alkaloids and other constituents of melicope semecarpifolia with antiplatelet aggregation activity. | three new quinoline alkaloids, 2-acetylevolitrine (1), 2-acetylpteleine (2), and semecarpifoline (3), along with 26 known compounds were isolated from the root bark of melicope semecarpifolia. the structures of 1-3 were elucidated by means of spectral analysis. in addition, (2s)-(--)-7,8-dimethoxyplatydesmine (4), cis-(+)-7,8-dimethoxymyrtopsine (5), and (3r)-(--)-8,9-dimethoxygeibalansine (6) were isolated as new natural products. several of these isolates were determined as exhibiting signific ... | 2001 | 11575945 |
| enrichment of the antibodies against the c-terminus of taiwan cobra cobrotoxin using dimeric glutaraldehyde-modified toxin as an immunogen. | the repertoire of antibodies producing by immunizing rabbits with cobrotoxin and dimeric glutaraldehyde-modified cobrotoxin (dga-cobrotoxin) was analyzed by studying the immunoreactivity of the two antibody preparations toward cobrotoxin, ga-cobrotoxin and recombinant cobrotoxin. the results of enzyme-linked immunoassay revealed that the two antibody preparations exhibited a higher reactivity against their cognate antigen. moreover, different behavior was observed for the reactivity of the two a ... | 2003 | 12565737 |
| the characterization of microsporidian isolates (nosematidae: nosema) from five important lepidopteran pests in taiwan. | microsporidian isolates from five lepidopteran pests-spodoptera litura, spodoptera exigua, helicoverpa armigera, plutella xylostella, and pieris spp.-were compared by spore morphology, infectivity to s. litura, western-blot banding patterns, the sequences of small subunit rrna gene (ssurrna sequence), and random amplified polymorphic dna polymerase chain reaction (rapd-pcr). all the isolates could infect experimentally and multiply in the larvae of s. litura. the s. exigua isolate showed the hig ... | 2003 | 12725812 |
| development of a recombinant protein-based enzyme-linked immunosorbent assay and its applications in field surveillance of rodent mice for presence of immunoglobulin g against orientia tsutsugamushi. | a recombinant protein containing the immunodominant conserved epitope region of the 56-kda outer membrane protein of the karp strain of orientia tsutsugamushi was purified to near homogeneity using recombinant dna techniques. the purified protein was used to immunize rabbits and produced an antibody that could recognize different strains of o. tsutsugamushi, as demonstrated both by western blotting and immunofluorescence assay. an enzyme-linked immunosorbent assay (elisa) based on this recombina ... | 2003 | 12738648 |
| characterization of new o3:k6 strains and phylogenetically related strains of vibrio parahaemolyticus isolated in taiwan and other countries. | we analysed the genetic divergence in the pandemic new o3:k6 and phylogenetically related (new o3:k6-like) strains and compare these two groups in terms of virulence and other biological traits. | 2005 | 15715859 |
| synthesis of baicalein derivatives as potential anti-aggregatory and anti-inflammatory agents. | the direct acylation of trimethoxyphenol (1) with substituted cinnamoyl chlorides followed by fries rearrangement and cyclization afforded a practical route for the synthesis of novel baicalein derivatives 4 functionalized on the b-ring in good overall yields. in the methylthiazoletetrazolium bromide (mtt) assay, none of the synthetic polyhydroxyflavonoids were cytotoxic at concentrations up to 200 microm on lipopolysaccharide (lps)-activated murine raw 264.7 macrophages over 24 h, while in the ... | 2005 | 15720786 |
| identification of oleosins as major allergens in sesame seed allergic patients. | the prevalence of sesame allergy is increasing in european countries. cases of severe allergy lack any evidence of specific immunoglobulin (ig)es by prick tests and capsystem-feia. the reasons for this negativity are unknown. | 2006 | 16436145 |
| sapinmusaponins f-j, bioactive tirucallane-type saponins from the galls of sapindus mukorossi. | five new tirucallane-type saponins, sapinmusaponins f-j (1-5), were isolated from the galls of sapindus mukorossi. the structures of these saponins were elucidated on the basis of spectroscopic analysis including 1d and 2d nmr techniques ((1)h-(1)h cosy, hmqc, hmbc, tocsy, and noesy). compounds 1-5 showed anti-platelet-aggregation effects, but no obvious cytotoxic activity for platelets as assayed by lactate dehydrogenase (ldh) leakage. compounds 1-5 also showed moderate activity in a 12-o-tetra ... | 2006 | 16724837 |
| therapeutic drug monitoring of cyclosporine neoral in de novo chinese cardiac transplant recipients treated with an everolimus-cyclosporine immunosuppressive regimen. | this study determined cyclosporine neoral (csa) pharmacokinetics and the accuracy of a limited sampling strategy to predict the 12-hour csa area-under-the-curve (auc) to provide a practical method for more accurate therapeutic drug monitor of csa among de novo chinese heart transplant recipients treated with an everolimus-csa immunosuppressive regimen. methods: blood samples were collected at 0, 0.5, 1, 2, 3, 4, 6, 8, 10, and 12 hours after oral administration of csa in six de novo heart recipie ... | 2006 | 16980022 |
| genetic and antigenic analyses of enterovirus 71 isolates in taiwan during 1998-2005. | enterovirus 71 (ev71) infections can lead to devastating clinical outcomes in children, with an increasing number of severe cases worldwide. the genetic and antigenic variability of ev71 strains isolated in taiwan in 1998-2005 was evaluated using partial nucleotide sequence analysis of the vp1 gene and the neutralisation assay. phylogenetic analyses revealed that most ev71 isolates from the 1998 epidemic belonged to sub-genogroup c2, with a minority belonging to sub-genogroup b4. between 1999 an ... | 2007 | 17488328 |
| treatment of angiostrongyliasis. | angiostrongyliasis, caused by angiostrongylus cantonensis, is endemic in northeastern thailand and southern and eastern taiwan and is also reported throughout the world. humans get infected by eating raw freshwater snails or other paratenic hosts. the three main clinical forms of angiostrongyliasis are: eosinophilic meningitis (eom), eosinophilic encephalitis (eoe) and ocular angiostrongyliasis. eom, the most common form, causes acute severe headache, and corticosteroid is the cornerstone treatm ... | 2008 | 18501934 |
| phylogenetic relationships of the g gene sequence of bovine ephemeral fever virus isolated in japan, taiwan and australia. | the g gene encoding the neutralization antigen of bovine ephemeral fever virus (befv) was characterized in order to define the virus's molecular epidemiology in japan and the genetic relationships among the japanese, taiwanese and australian isolates. the nucleotide and amino acid sequences of the gene were highly conserved among the japanese strains, regardless of the year of isolation, and were closely related to the taiwanese strains. by phylogenetic analysis, the japanese and taiwanese strai ... | 2009 | 19201554 |
| molecular characterization of dermatophytes of the trichophyton mentagrophytes complex found in taiwan with emphasis on their correlation with clinical observations. | fungi of the trichophyton mentagrophytes complex are important pathogenic dermatophytes capable of causing various human skin infections. there are many species and varieties involved in this complex. due to morphological similarity and existing intermediate forms and variants, unequivocally separating these dermatophytes is not always straightforward, and sampling appropriate isolates for research is often troublesome. communicating epidemiological statistics of these dermatophytes is greatly i ... | 2010 | 20649795 |
| identification of the dc-sign-interactive domains on the envelope glycoprotein of hiv-1 crf07_bc. | dc-sign, a c-type lection expressed on dendritic cells, enhances hiv-1 infection in cis and in trans. hiv-1 circulating recombinant form (crf) 07_bc viruses have been the predominant strain found among injection drug users in southern china and taiwan. the goal of this study was to map the dc-sign-interactive domain on the gp120 of crf07_bc. pseudotyped viruses containing single (n233q, n275q, n330q, n351q, n355q, n381q, and n387q), double (n233q + n275q, n233q + n351q, n275q + n351q), or triple ... | 2011 | 21142800 |
| expression and prognostic significance of gastric-specific annexin a10 in diffuse- and intestinal-type gastric carcinoma. | annexin a10 (anxa10) and its liver-specific short isoform (anxa10s) had tissue-restricted expression. the downregulation of anxa10s is correlated with tumor progression and poor prognosis in hepatocellular carcinoma. the aim of the present study was to validate the tissue distribution and explore the role of the anxa10 protein expression in gastric carcinoma. | 2011 | 21175800 |
| prevalence, infectivity and oocyst sporulation time of rabbit-coccidia in taiwan. | prevalence of eimeria species parasitizing rabbits (oryctolagus cuniculus) in taiwan were investigated. six eimeria species, namely: eimeria media (prevalence, 158/642; 24.6%), eimeria magna (101/642; 15.7%), eimeria perforans (58/642; 9.0%), eimeria coecicola (46/642; 7.2%), eimeria piriformis (16/642; 2.5%), and eimeria exigua (9/642; 1.4%) were observed. the overall prevalence of these coccidial infections in rabbits from pet shops and farms was 46.2% and 41.7%, respectively. concurrent infec ... | 2010 | 21399582 |