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prevalence, disease associations and risk factors for colonization with intestinal spirochaetes (brachyspira spp.) in flocks of laying hens in north-eastern italy.the present study investigated the occurrence of anaerobic intestinal spirochaetes of the genus brachyspira in laying hen flocks in treviso province, north-eastern italy, with respect to prevalence, spirochaete species present, disease associations and risk factors for colonization. a total of 450 faecal samples from 45 sheds on 29 laying hen farms were cultured for intestinal spirochaetes. nineteen sheds on 12 farms contained chickens with symptoms consistent with avian intestinal spirochaetosi ...200818568654
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