| studies on canine babesiosis in okinawa island. | | 1984 | 6492541 |
| clinical observations on babesia gibsoni and babesia canis infections in dogs. | thirty-five cases of babesia gibsoni infection and 11 cases of babesia canis infection were diagnosed and treated in dogs at kadena air base in okinawa, japan, between april 1979 and february 1980. diagnosis was made by demonstrating the organisms in blood smears and by serologic examination, using an indirect fluorescent antibody test. serologic findings correlated well with the occurrence of infection in mature dogs, but poorly in young (1- to 3-month-old) dogs. although these 2 intraerythrocy ... | 1982 | 7061333 |
| recent increase of human infections with dog heart worm dirofilaria immitis in japan. | the human infection with dirofilaria immitis, was reported for the first time in 1964 from a subcutaneous case, and in 1968 from a pulmonary case in japan. since that time, a total of human d. immitis cases amounted to 120 cases up to the first half of 1995. among these, only one infection with d. repens was reported from a southern island of okinawa. the parasitological aspects of these cases are reviewed along with clinical, pathological, immunological and epidemiological features. | 1997 | 9802097 |
| there are at least three genetically distinct small piroplasms from dogs. | the 18s nuclear subunit ribosomal rna (18s rrna) gene of small piroplasms isolated from dogs from okinawa (japan), oklahoma, north carolina, indiana, missouri, and alabama, was isolated and sequenced. phylogenetic analysis of these sequences and comparisons with sequences from other babesia, cytauxzoon, and theileria species revealed that all canine small babesial isolates, with the exception of isolates from california and spain, were placed in a group containing the babesia spp. sensu stricto. ... | 2000 | 11428342 |
| a nationwide survey of ixodid tick species recovered from domestic dogs and cats in japan in 2011. | a nationwide survey of ixodid ticks was performed in 2011, during which a total of 4237 and 298 ticks were recovered from 1162 dogs and 136 cats, respectively. haemaphysalis longicornis was the most frequently found tick species on canine hosts (739 dogs), followed by h. flava (166), ixodes ovatus (139), and rhipicephalus sanguineus sensu lato (70). h. hystricis, h. japonica, h. megaspinosa, h. formosensis, h. campanulata, h. ias, i. nipponensis, i. persulcatus, and amblyomma testudinarium were ... | 2014 | 25155339 |
| epidemiological survey of babesia species in japan performed with specimens from ticks collected from dogs and detection of new babesia dna closely related to babesia odocoilei and babesia divergens dna. | detection and analysis of babesia species from ticks recovered from dogs in japan were attempted by pcr and nucleotide sequence analysis based on the 18s rrna gene, respectively. a total of 1136 ticks were examined for babesia dna by 18s rrna-based pcr and nucleotide sequencing. partial sequences of babesia canis vogeli dna were detected from six ticks in aomori, nara, hiroshima, oita, and okinawa prefectures; and babesia gibsoni asia-1 dna was also detected in four ticks in osaka, hiroshima, mi ... | 2003 | 12904344 |
| ixodid tick species recovered from domestic dogs in japan. | the species of ixodid ticks (acari: ixodidae) recovered from domestic dogs in japan between september to november 2000 and april to june 2001 were identified. a total of 4122 ticks, including 1624 larvae, 1200 nymphs, 1016 females and 282 males were removed from 1221 dogs during these periods. haemaphysalis longicornis (neumann) was the most frequently found (40.3% of dogs), followed by h. flava (neumann) (16.1% of dogs), rhipicephalus sanguineus (latreille) (4.8% of dogs) and ixodes ovatus (neu ... | 2003 | 12680923 |
| demonstration of anaplasma (ehrlichia) platys inclusions in peripheral blood platelets of a dog in japan. | a free-roaming dog in okinawa island showed anaplasma (ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. the inclusions were positive in indirect fluorescence test with anti-a. phagocytophila serum. the platelet count of the dog was 170,000 microl(-1). the sequence analysis of the 16s rrna, citrate synthase and heat shock protein genes of dna from the infected platelets confirmed that the inclusions were a. platys. this is the first detection of a. platys inclus ... | 2002 | 12446100 |
| characterization of spotted fever group rickettsiae detected in dogs and ticks in okinawa, japan. | spotted fever group (sfg) rickettsial dnas were detected in 2.4% of 340 canine blood samples and a pool of 84 tick pool samples (229 ticks) collected in okinawa, japan by pcr using a citrate synthase and an sfg rickettsial 190-kda surface antigen gene primer pair. the sequences of both genes from canine blood and tick samples showed high levels of similarity with those of rickettsiajaponica and several sfg rickettsiae (r. aeschlimannii, r. massiliae, r. rhipicephali and bar-29 strain). phylogene ... | 2002 | 12061628 |
| [the population history of the japanese]. | this paper introduces a "dual structure model" that explains the population history of the japanese population including the okinawa islanders (ryukyus) and ainu under a single hypothesis. the model assumes that the first occupants of the japanese archipelago came from somewhere in southeast asia in the upper palaeolithic age and gave rise to the people in the neolithic jomon age, or jomonese; then the second wave of migration from north asia took place in and after the aeneolithic yayoi age; an ... | 1993 | 8295350 |
| incidence of brown dog ticks, rhipicephalus sanguineus, at a kennel in okayama prefecture. | twenty two adult dogs suffered from tick infestation at a kennel in okayama prefecture in the summer of 1994. four of them had been introduced from u.s.a. some dogs showed pyoderma, anemia, neutrophilia or eosinophilia. neither babesia gibsoni nor hepatozoon canis was detected on the smear samples of peripheral blood. the ticks detected were morphologically identified as rhipicephalus sanguineus. this case was a rare incidence of the tick in the other areas of japan than okinawa. | 1995 | 7548423 |
| the recession of gastric cancer and its possible causes. | the paper re-examines the hypothesis that excessively hot drinks constitute an important risk factor in the causation of gastric cancer. the recession of gastric cancer mortality rates in the united states in recent decades is attributed to dietary changes tending to supplant the traditional hot beverages. one such change was the appearance of domestic refrigerators, promoting iced drinks, another the popularisation of soft drinks. the example of okinawa is quoted where in 1972, after 27 years o ... | 1978 | 634179 |
| detection of rickettsial dna in ixodid ticks recovered from dogs and cats in japan. | dna from ticks recovered from 1137 dogs and 133 cats from all over japan were examined for rickettsia infection by citrate synthase gene (glta)-based pcr and partial nucleotide sequencing. a total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested pcr. sequence analysis was successfully performed on 102 samples. dna of rickettsia japonica or closely related rickettsia spp. strains were detected from 38 ticks in 16 prefectures mainly in weste ... | 2005 | 16397379 |
| serodiagnosis of babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated p50. | the surface antigen p50 of babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. in order to establish an effective diagnostic method for practical use, the gene encoding truncated p50 (p50t) lacking a signal peptide and c-terminal hydrophobic regions were cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase (gst). more than 90% portion of the gst-p50t was expressed as a soluble form, in contrast wit ... | 2004 | 15644601 |
| detection of ehrlichial infection by pcr in dogs from yamaguchi and okinawa prefectures, japan. | species-specific nested polymerase chain reaction (pcr) was used to detect the presence of possible canine ehrlichial agents (ehrlichia canis, e. chaffeensis, e. ewingii, e. equi and e. platys) and monocytic ehrlichial agents found in japan (e. muris and a recently discovered ehrlichia species detected from ixodes ovatus) in blood samples from dogs in yamaguchi and okinawa prefecture, japan. partial sequence of e. platys was detected from 1 of 67 dogs (1.5%) tested from yamaguchi prefecture and ... | 2001 | 11503912 |
| serological survey of neospora caninum infection among dogs in japan through species-specific elisa. | a seroepidemiological survey of neospora caninum infection among dogs in japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (nc-sag1t). among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from hokkaido to okinawa were positive to n. caninum infections, which were more frequently detected in females than males. siberian huskies showed the highest positive rate compared with the other breeds. dogs with pyometra and diabetes mel ... | 2008 | 18772569 |
| detection of borrelia garinii, borrelia tanukii and borrelia sp. closely related to borrelia valaisiana in ixodes ticks removed from dogs and cats in japan. | ticks removed from 1136 dogs and 134 cats all over japan were examined for borrelia infection by pcr and sequencing. the 5s-23s rdna intergenic spacer of borrelia was detected from two ixodes persulcatus ticks from two dogs and two unidentified ixodes spp. from another two dogs in hokkaido, and two ixodes granulatus ticks from two cats in okinawa. additional 2 i. granulatus from the same cats also showed positive. sequence analysis of the pcr products revealed that the one from hokkaido was simi ... | 2007 | 17049744 |
| microsatellite polymorphism in japanese mongrel dogs. | the genetic variability of 182 unrelated mongrel dogs living in various areas of japan (from hokkaido to okinawa) was studied by collecting their blood. ten microsatellite loci were chosen from different autosomal chromosomes. after combining a few rare adjoining alleles to allelic classes, it was confirmed that the hardy-weinberg equilibrium was attained in each locus. the polymorphic information contents (pics) of the loci, ren37a11, ren48e01, ahtk253, zubeca30, ren277k09, ren42n13, aht130, pe ... | 2005 | 16276064 |
| molecular survey of babesia infection in dogs in okinawa, japan. | a total of 80 free-roaming dogs on okinawa island, japan, were examined for babesia infection using the polymerase chain reaction (pcr) and sequence analysis. of 80 samples, 12 were positive in a babesia genus-specific pcr. consequent species-specific pcr for b. canis and b. gibsoni revealed that 5 (6.3%) and 7 (8.8%) dogs were infected with b. canis and b. gibsoni, respectively. sequence analysis of the pcr products revealed that the 18s rrna gene sequence of b. canis detected from dogs in okin ... | 2004 | 15135876 |
| analysis of 16s rrna gene sequences of ehrlichia canis, anaplasma platys, and wolbachia species from canine blood in japan. | in the present study, three canine blood samples from japan, that were suspected to be ehrlichia positive were examined. after sequencing the 16s rrna genes, each dog was found to be infected either with ehrlichia canis (kagoshima 1), anaplasma platys (okinawa 1), or wolbachia sp. (okinawa 2). phylogenic analysis was performed on these sequences. the nearly entire 16s rrna sequence of kagoshima 1 was found to be most similar to the sequences from oklahoma and venezuela e. canis strains (1 base p ... | 2003 | 12860708 |
| first detection of ehrlichia platys in dogs and ticks in okinawa, japan. | we investigated ehrlichia platys infection of dogs and ticks in okinawa, japan. using e. platys specific primers, e. platys and he3-r, pcr-positive results were obtained with 32.0% (64/200) of blood samples of dogs and 3.8% (3/77) of ticks. the nucleotide sequences of the amplified dna fragment from the dogs and the ticks infesting them were identical, and the sequence corresponded to that of the e. platys gzh981 strain. we concluded that there is a cyclic maintenance of e. platys between dogs a ... | 2001 | 11270612 |
| seroprevalence of antibodies against spotted fever group rickettsia among dogs and humans in okinawa, japan. | we evaluated serum antibodies against rickettsia japonica in 517 dogs (430 stray dogs and 87 pet dogs) and 164 humans in okinawa, japan, by indirect immunofluorescence assay. the seropositive rate in stray dogs was significantly higher than that in pet dogs (30.7 versus 4.6%, p<0.01). this high prevalence rate is attributed to the understandably frequent environmental exposure of stray dogs to tick infestation. human samples obtained from okinawa and sapporo also showed a significant difference ... | 2001 | 11270611 |
| detection of ehrlichia platys dna in brown dog ticks (rhipicephalus sanguineus) in okinawa island, japan. | thirty-two rhipicephalus sanguineus females collected from eight free-roaming dogs in okinawa island, japan, were examined for ehrlichial dna by 16s rrna-based pcr and subsequent sequencing. partial sequences of ehrlichia platys 16s rrna (678 to 679 bp) were detected in three ticks (9.4%) from two dogs. this is the first report of detection of e. platys in japan, and also the first report of detection in ticks. | 2000 | 11060094 |
| distribution of leptospirosis among stray dogs in the okinawa islands, japan: comparison of the microcapsule and microscopic agglutination tests. | three hundred and thirty-eight sera collected from stray dogs in the okinawa islands were examined for antibodies against leptospira interrogans using the microscopic agglutination test (mat) and the one-point microcapsule agglutination test (mcat). seventy-eight sera (23%) showed a positive reaction to at least one of the six serovar antigens, and 69 of these reacted with serovar canicola by microcapsule agglutination test. the mixed microcapsule agglutination test detected 68 of the microscopi ... | 1989 | 2790440 |