epidemiological survey of babesia species in japan performed with specimens from ticks collected from dogs and detection of new babesia dna closely related to babesia odocoilei and babesia divergens dna. | detection and analysis of babesia species from ticks recovered from dogs in japan were attempted by pcr and nucleotide sequence analysis based on the 18s rrna gene, respectively. a total of 1136 ticks were examined for babesia dna by 18s rrna-based pcr and nucleotide sequencing. partial sequences of babesia canis vogeli dna were detected from six ticks in aomori, nara, hiroshima, oita, and okinawa prefectures; and babesia gibsoni asia-1 dna was also detected in four ticks in osaka, hiroshima, mi ... | 2003 | 12904344 |
serodiagnosis of babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated p50. | the surface antigen p50 of babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. in order to establish an effective diagnostic method for practical use, the gene encoding truncated p50 (p50t) lacking a signal peptide and c-terminal hydrophobic regions were cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase (gst). more than 90% portion of the gst-p50t was expressed as a soluble form, in contrast wit ... | 2004 | 15644601 |
detection of antibodies against japanese encephalitis virus in raccoons, raccoon dogs and wild boars in japan. | japanese encephalitis virus (jev) infects numerous animal species including humans, horses and pigs. in this study, antibodies against jev in feral raccoons (procyon lotor), wild boars (sus scrofa) and raccoon dogs (nyctereutes procyonoides) in japan were examined. the results showed that 40.7% (22 out of 54), 64.5% (40 out of 62), 69.1% (47 out of 68) and 0% (0 out of 20) of raccoons in hyogo, osaka, wakayama and hokkaido, respectively, had virus-neutralizing antibodies against jev. in addition ... | 2009 | 19721354 |
cryptosporidium infection in dogs in osaka, japan. | cryptosporidium parvum is a zoonotic pathogen composed of genetically distinct but morphologically identical genotypes. recent molecular study indicates that dogs may transmit the cattle genotype, which is known to be pathogenic to humans. although large-scale studies of cryptosporidium infection in dogs have been performed in several countries, the isolates were not accurately identified because of the lack of a method for molecular analysis. it is important to identify the isolates harbored in ... | 2002 | 12237137 |
a survey of blastocystis sp. in livestock, pets, and zoo animals in japan. | the prevalence of blastocystis sp. was examined in fecal samples collected from cattle, pigs, dogs, and a variety of zoo animals (primates, carnivores, herbivores, pheasants, and ducks) by direct observation of fresh fecal suspensions or cultured materials, using light microscopy. the cattle and pigs were randomly sampled from 11 and 12 commercial farms, respectively, located in the western region of japan. the dog material used in this study was obtained from pets housed in an animal shelter in ... | 2002 | 12062509 |
incidence of otitis externa in dogs and cats in japan. | the incidence of otitis externa in dogs and cats admitted to the animal hospital of the university of osaka prefecture was investigated and the bacteria isolated were tested for antibiotic susceptibility. of the various breeds examined, the incidence of otitis externa was highest in miniature poodles and cocker spaniels and himalayan and persian cats. the organisms most commonly associated with otitis externa were coagulase-negative staphylococci, followed by coagulase-positive staphylococci, st ... | 1981 | 7027593 |
Prevalence of Corynebacterium ulcerans in dogs residing in Osaka, Japan. | Diphtheria-like human illness caused by Corynebacterium ulcerans is an emerging threat in developed countries, with incidence sometimes higher than that of diphtheria caused by Corynebacterium diphtheriae. Companion animals are considered a potential source of human infections. In order to determine the prevalence of C. ulcerans among dogs, we performed a screening for the bacterium in 583 dogs in the custody of the Osaka Prefectural government. Forty-four dogs (7.5%) were positive for the bacte ... | 2011 | 21921111 |
survey and molecular characterization of cryptosporidium and giardia spp. in owned companion animal, dogs and cats, in japan. | compared with other countries, surveys of these parasites have been rarely performed in companion animals of japan in spite of their significance for public health. here, we investigated pet dogs and cats in japan for the first time, and genetically analyzed the isolates to evaluate the risk of zoonotic infections. seventy-seven fecal samples were collected from privately owned dogs and 55 samples from owned cats in osaka city, japan. cryptosporidium oocysts were identified in 3/77 dogs (3.9%) a ... | 2010 | 20934255 |
breastfeeding and atopic eczema in japanese infants: the osaka maternal and child health study. | epidemiological studies associated with breastfeeding have provided conflicting results about whether it is preventive or a risk factor for atopic eczema in children. the current prospective study investigated the relationship between breastfeeding and the risk of atopic eczema in japan. a birth cohort of 763 infants was followed. the first survey during pregnancy and the second survey between 2 and 9 months postpartum collected information on potential confounding factors and atopic eczema stat ... | 2009 | 19438982 |
breastfeeding and the risk of wheeze and asthma in japanese infants: the osaka maternal and child health study. | epidemiological evidence for an effect of breastfeeding on asthma continues to be inconclusive. the present prospective study examined the relationship between breastfeeding and the risk of wheeze and asthma in japanese infants. a birth cohort of 763 infants was followed. the first survey during pregnancy and the second survey between 2 and 9 months postpartum collected information on potential confounding factors. data on breastfeeding, wheeze, and asthma were obtained from questionnaires in th ... | 2008 | 18266830 |
survey of cryptosporidium spp. and giardia spp. infections in various animals at a zoo in japan. | a total of 284 fecal samples of 89 species (43 mammalian species and 46 avian species) were examined for cryptosporidium oocysts and giardia cysts from 1999 to 2002. each sample was collected at the zoo located at osaka in japan and examined by microscopy after performing the sucrose flotation method and by two immunofluorescent assay kits for detection of cryptosporidium oocysts and giardia cysts. cryptosporidium spp. was found only in a raccoon dog (nyctereutes procyonoides), and giardia spp. ... | 2005 | 17323580 |