| diagnosis of brazilian vesiculoviruses by reverse transcription-polymerase chain reaction. | we describe a reverse transcription-polymerase chain reaction (rt-pcr) and a nested-pcr for diagnosis of piry, carajás, cocal, and alagoas vesiculoviruses from brazil. the rna extracts of viral and clinical samples were submitted to a rt-pcr using vesiculovirus g primers that amplify part of the glycoprotein gene. the rt-pcr produced amplicons of expected size, 290 base pair, for the four studied viruses. the rt-pcr showed a high sensitivity being 151.3 times (2.18 log) more sensitive for the de ... | 2005 | 16021308 |
| characterization of the full-length genomic sequences of vesicular stomatitis cocal and alagoas viruses. | in brazil and argentina, vesicular stomatitis (vs) is caused by distinct viral strains serologically related to the classical vesicular stomatitis virus indiana (vsiv), namely vs indiana-2 (vsiv-2) and vs indiana-3 (vsiv-3). here we describe the full-length genomic sequences and organization of the prototype strains of vsiv-2 cocal virus (cocv) and vsiv-3 alagoas virus (vsav). these viruses showed similar genomic organizations to vsiv field isolates except that the non-structural c'/c proteins, ... | 2008 | 18512001 |