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metschnikowia hawaiiensis sp. nov., a heterothallic haploid yeast from hawaiian morning glory and associated drosophilids.a new haploid, heterothallic yeast species was isolated repeatedly from morning glory (ipomoea acuminata) flowers and from two associated drosophilid species, scaptomyza calliginosa and drosophila floricola, in a hawaiian kipuka. haploid strains of this organism multiply asexually by budding and, under nutrient deprivation, by the formation of long germ tubes that develop into branching true mycelia. mating compatibility is controlled by two alleles of a single locus. plasmogamy between compatib ...19902275857
gastrostomy tube infections in a community hospital.percutaneous gastric feeding tubes are becoming increasingly more common to provide nutrition in debilitated patients, while they decrease the risk of aspiration associated with nasogastric tubes.19968902109
pichia lachancei sp. nov., associated with several hawaiian plant species.a description is given of pichia lachancei sp. nov., a new species of yeast that occurs in association with several hawaiian plant species of the genera tetraplasandra, cheirodendron and clermontia. the new species is heterothallic and occurs in nature in the haploid as well as the diploid state. upon conjugation of complementary mating types, zygotes are formed that reproduce by budding as diploid cells. when placed on sporulation medium, four hat-shaped spores are produced which are rapidly re ...199910425793
on the biogeography of yeasts in the wickerhamiella clade and description of wickerhamiella lipophila sp. nov., the teleomorph of candida lipophila.we describe the new yeast species wickerhamiella lipophila, the teleomorph of candida lipophila, a haploid heterothallic yeast previously isolated from insects associated with morning glories in hawaii. both mating types were recovered in the eastern region of maui, and a single strain was found in the waimea region of kauai. we reexamined the mating compatibility of the several strains of candida lipophila previously collected on the island of hawaii and found them to be fertile mating types th ...200011142405
metschnikowia lochheadii and metschnikowia drosophilae, two new yeast species isolated from insects associated with flowers.two new haplontic heterothallic species of metschnikowia were isolated from floricolous insects and flowers. metschnikowia lochheadii was recovered from insects found in various flowers on the hawaiian islands of kauai and maui, and from conotelus sp. (coleoptera: nitidulidae) in northwestern guanacaste province, costa rica. the morphology, physiology, and sexual cycle are typical of the large-spored metschnikowia species, and the partial ribosomal dna large subunit (d1d2) sequences suggest that ...200111261488
biogeography of the yeasts of ephemeral flowers and their insects.we studied specific yeast communities vectored by beetles, drosophilids, and bees that visit ephemeral flowers, mostly in the genus hibiscus and in the families convolvulaceae and cactaceae, in the neotropical, nearctic, and australian biogeographic regions. the communities consist mostly of yeasts in four clades centered around the genera metschnikowia, kodamaea, wickerhamiella, and starmerella. the largest geographic discontinuity occurs as a function of the nitidulid beetle species that domin ...200112702457
hanseniaspora meyeri sp. nov., hanseniaspora clermontiae sp. nov., hanseniaspora lachancei sp. nov. and hanseniaspora opuntiae sp. nov., novel apiculate yeast species.fourteen apiculate yeast strains isolated from various sources in south africa, north america and the hawaiian islands were found to be genetically divergent from other hanseniaspora-kloeckera species by using randomly amplified polymorphic dna (rapd)-pcr. after cluster analysis of the rapd-pcr fingerprints, five groups were recognized. dna reassociation values among representatives of these groups and strains of hanseniaspora-kloeckera species revealed that the strains represent five novel spec ...200313130067
geography and niche occupancy as determinants of yeast biodiversity: the yeast-insect-morning glory ecosystem of kīpuka puaulu, hawai'i.biodiversity theory proposes two types of hypotheses to account for the species composition of a given community. the first encompasses geographic and historical factors. for example, local species richness is thought to be affected by area, proximity to large landmasses, dispersal mechanisms, and climatic history, collectively known as biogeography. the second type, termed niche occupancy rules, deals with the intrinsic properties of the species as they affect their interaction with the habitat ...200314554202
candida leandrae sp. nov., an asexual ascomycetous yeast species isolated from tropical plants.the novel yeast species candida leandrae is described based on eight isolates from decaying fruits of leandra reversa cogn. (melastomataceae) in an atlantic rainforest site in brazil, one from a convolvulaceae flower in costa rica and one from a drosophilid in hawai'i. the strains differed in their colony morphology, one being butyrous and smooth and the other being filamentous and rugose. sequences of the d1/d2 domains of the large-subunit rrna gene from both morphotypes were identical. c. lean ...200415545490
metschnikowia hamakuensis sp. nov., metschnikowia kamakouana sp. nov. and metschnikowia mauinuiana sp. nov., three endemic yeasts from hawaiian nitidulid beetles.three heterothallic, haplontic yeast species, metschnikowia hamakuensis, metschnikowia kamakouana and metschnikowia mauinuiana, are described from isolates associated with endemic nitidulid beetles living on various endemic plants on three hawaiian islands. as morphospecies, they are similar to metschnikowia hawaiiensis, but based on mating compatibility and ascospore formation, they can be assigned clearly to distinct biological species. analysis of its/5.8s and d1/d2 large subunit rdna sequenc ...200515879284
penicillium species endophytic in coffee plants and ochratoxin a production.tissues from coffea arabica, c. congensis, c. dewevrei and c. liberica collected in colombia, hawaii and at a local plant nursery in maryland were sampled for the presence of fungal endophytes. surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. dna was extracted from a set of isolates visually recognized as penicillium, and the internal transcribed spacer region and partial lsu-rdna was amplified and sequenced. comparison of dna sequ ...200616800302
molecular-genetic diversity of the ascomycetous yeast genus arthroascus: arthroascus babjevae sp. nov., arthroascus fermentans var. arxii var. nov. and geographical populations of arthroascus schoenii.using molecular and genetic analyses, 28 arthroascus strains were analysed, isolated from widely different geographical localities in europe, north america, far-east asia and hawaii. most of the strains have been assigned to the species arthroascus schoenii. pcr-rapd revealed two japanese arthroascus strains (ucd 67-278 and ifo 10138) to have peculiar patterns. comparative rdna (d1/d2 26s, its1 and its2) sequence analysis showed that the two strains respectively represent a novel species and a n ...200616902043
metschnikowia orientalis sp. nov., an australasian yeast from nitidulid beetles.a novel species, metschnikowia orientalis sp. nov., is described for haploid, heterothallic yeasts isolated from nitidulid beetles sampled in flowers in rarotonga in the cook islands, and the cameron highlands of malaysia. as evidenced by analysis of d1/d2 large subunit rdna sequences, the species is related to candida hawaiiana, to which it is similar in growth responses. cylindrical, conjugated asci and acicular ascospores of moderate size are formed. rudimentary mating reactions were observed ...200617012584
biogeography and population structure of the neotropical endemic yeast species metschnikowia lochheadii.the genetic structure of populations of the out-crossing haplontic yeast species metschnikowia lochheadii was investigated. the species is associated with floricolous beetles in central america and hawaii. the objective was to determine whether sexual reproduction is prevalent and to what extent the geographic distribution of genotypes can be viewed as historical. the genetic markers examined include the mating type (h (+) or h (-)) and nine polymorphic dna loci. the data were used to assess pop ...200818536979
sympodiomycopsis lanaiensis sp. nov., a basidiomycetous yeast (ustilaginomycotina: microstromatales) from marine driftwood in hawai'i.a previously unknown basidiomycetous yeast is described for which the name sympodiomycopsis lanaiensis is proposed. the type strain, lm418(t), was isolated from driftwood collected on a beach on lana'i (hawai'i). on the basis of ribosomal dna sequence analysis [large subunit (lsu), internal transcribed spacer (its) 1 & 2, and 18s], lm418(t) belongs to the order microstromatales, which includes the genera sympodiomycopsis, rhodotorula, microstroma, volvocisporium and quambalaria. the strain is de ...200819054136
population structure of two beetle-associated yeasts: comparison of a new world asexual and an endemic nearctic sexual species in the metschnikowia clade.the genetic structure of two related yeast species, one sexual and one asexual, was compared using polymorphic dna markers. although both yeasts propagate by asexual budding of haploid cells, metschnikowia borealis reproduces sexually when compatible strains come in contact. to what extent this has occurred in nature was not known. as candida ipomoeae is a closely related, asexual species, the two yeasts provide an excellent model system to assess the role of sexual reproduction in a biogeograph ...200919319659
cryptococcus gattiivgi in a spinner dolphin (stenella longirostris) from hawaii.a spinner dolphin (stenella longirostris) was found stranded in hawaii with cutaneous nodules and enlarged lymph nodes. numerous cryptococcus gattii vgi yeast were observed in multiple organs with minimal inflammation. this case represents the first reported infection of c. gattii in a dolphin from hawaii.201020722278
lehualides e-k, cytotoxic metabolites from the tongan marine sponge plakortis sp.spectroscopy-guided chemical analysis of a marine sponge from the genus plakortis, collected in tonga, yielded seven new metabolites of polyketide origin, lehualides e-k (5-11), four of which incorporate various sulfur functionalities. the structures of compounds 5-11 were elucidated by interpretation of spectroscopic data and spectral comparison with model compounds. the biological activities of compounds 6-9 were investigated against human promyeloid leukemic hl-60 cells and two yeast strains, ...201121351759
citeromyces hawaiiensis sp. nov., a new ascosporic yeast associated with myoporum sandwicense in hawaii.citeromyces hawaiiensis sp. nov. (nrrl y-11581, cbs 12303, type strain) is described from 12 strains isolated from flux of the sandalwood (myoporum sandwicense) and adjacent soil in hawaii, usa. analysis of gene sequences from the d1/d2 domains of nuclear large subunit rrna, its, mitochondrial small subunit rrna and translation elongation factor-1α each separated the proposed new species from c. matritensis and c. siamensis, the other known species of the genus citeromyces. the three species are ...201121828013
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