| journey of a molecular biologist. | my journey into a research career began in fermentation biochemistry in an applied science department during the difficult post-world war ii time in japan. subsequently, my desire to do research in basic science developed. i was fortunate to be a postdoctoral fellow in the united states during the early days of molecular biology. from 1957 to 1960, i worked with three pioneers of molecular biology, sol spiegelman, james watson, and seymour benzer. these experiences helped me develop into a basic ... | 2010 | 21456966 |
| sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process. | the juice from sweet sorghum cultivar sil-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. nanofiltration (nf) of an ultrafiltration (uf) permeate using an ntr-7450 membrane (nitto denko corporation, osaka, japan) concentrated the juice and produced a su ... | 2017 | 28488116 |
| detection of retinoic acid receptor agonistic activity and identification of causative compounds in municipal wastewater treatment plants in japan. | retinoic acid (ra) receptor (rar) agonists are potential toxicants that can cause teratogenesis in vertebrates. to determine the occurrence of rar agonists in municipal wastewater treatment plants (wwtps), we examined the rarĪ± agonistic activities of influent and effluent samples from several municipal wwtps in osaka, japan, using a yeast two-hybrid assay. significant rarĪ± agonistic activity was detected in all the influent samples investigated, suggesting that municipal wastewater consistently ... | 2011 | 22095885 |
| detection of genistein as an estrogenic contaminant of river water in osaka. | the estrogenic activity in water at various localities on lake biwa-yodo river, a representative watershed in japan, was measured using a recombinant yeast that expresses the human estrogen receptor. the yeast bioassay revealed that the activities of 13 water samples had an average value of 14 pmol/l (3.8 ng/l) (17beta-estradiol equivalent) with a very wide range from 0 to 72 pmol/l (0-19.6 ng/ l), and two of the samples had prominent levels of activity (72 pmol/l (19.6 ng/l) and 56 pmol/l (15.2 ... | 2004 | 15597900 |