| a quantitative taqman pcr assay for the detection of mycoplasma suis. | aim: to develop a taqman probe-based, highly sensitive and specific quantitative pcr (qpcr) assay for the detection and quantification of m. suis in the blood of pigs. methods and results: primers and probes specific to m. suis 16s rrna gene were designed. the qpcr assay's specificity, detection limit, intra- and inter-assay variability were evaluated and its performance was compared to a m. suis conventional pcr assay (cpcr). blood of two experimentally infected pigs, 40 indiana pigs, 40 braz ... | 2011 | 21575112 |