| a cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (brachyspira spp.) in three flocks of laying hens. | a cross-sectional study was conducted on a commercial egg-producing farm with a history of wet litter. a total of 600 fresh caecal faecal samples were obtained from under cages of laying hens in three sheds each containing flocks of approximately 5400 hens. samples were cultured for intestinal spirochaetes, and growth on the primary isolation plate was observed under a phase contrast microscope and subjected to pcrs specific for the intestinal spirochaetes brachyspira intermedia and brachyspira ... | 2004 | 15708815 |