| [molecular analysis of salmonella enteritidis by pulsed-field gel electrophoresis]. | to study the relationship between different strains of salmonella enteritidis, a molecular epidemiologic analysis method-pulsed-field gel electrophoresis was established and salmonella enteritidis isolated from food samples were tested. | 2005 | 16111047 |
| vaccines developed for h5 highly pathogenic avian influenza in china. | since the first detection of highly pathogenic h5n1 avian influenza virus from sick goose in guangdong province in china in 1996, scientists in china started to develop vaccines for avian influenza pandemic preparedness. an h5n2 inactivated vaccine was produced from a low pathogenic virus, a/turkey/england/n-28/73, and was used for the buffer zone vaccination in the h5n1 outbreaks in 2004 in china. we also generated a low pathogenic h5n1 reassortant virus a/harbin/re-1/2003 (re-1) that derives i ... | 2006 | 17135510 |
| [monitoring influenza a virus and newcastle disease virus in migratory waterfowls in sanjiang natural reserve of heilongjiang province]. | in order to monitor the present situation of avian influenza virus (aiv) and newcastle disease virus (ndv) in migratory waterfowls effectively, 158 tracheal and cloacal swab samples for wild birds were collected from sanjiang natural reserve during migratory seasons in october 2005, april 2006 and october 2006. | 2008 | 19160826 |
| temporal and spatial characteristics of highly pathogenic avian influenza outbreaks in china during 2004 to 2015. | identifying the temporal and spatial characteristics of highly pathogenic avian influenza (hpai) outbreaks is very important for developing effective and appropriate countermeasures against hpai and promoting sustainable development in the poultry industry. this study aimed to analyze four aspects of the temporal and spatial characteristics of hpai outbreaks in china, including the frequency of hpai outbreaks, numbers of dead animals (died or culled), types of hpai viruses, and species of infect ... | 2017 | 28453859 |
| sequence and phylogenetic analysis of a chinese very virulent infectious bursal disease virus. | the complete genome sequence of a chinese very virulent infectious bursal disease virus (vvibdv) strain, harbin-1, was determined. based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvibdv strains were identified. phylogenetic analysis indicated that a reassortment and/or recombination event may have occurred in the emergence of chinese vvibdv strains. | 2008 | 18622570 |
| assessment of genetic, antigenic and pathotypic criteria for the characterization of ibdv strains. | the aim of this work was the selection and comparison of representative infectious bursal disease virus (ibdv) strains. nine strains of ibdv, isolated at different times and from different geographic regions of europe and china, were characterized. batches of all strains were prepared following standardized protocols and checked for the absence of contaminating viruses. criteria used for their characterization were: (i) the nucleotide sequence of the vp2 variable region, (ii) binding to a panel ... | 2004 | 15545026 |
| [gene cloning and sequencing of chicken anemia virus(cav) isolated from harbin]. | a chicken anemia virus has been isolated from a chicken flock in harbin of china. the genome of the ivrus was cloned through polymerase chain reaction(pcr) and sequence of the genome was analyzed. the cycle genome is made of 2298 base pairs including three overlapping open reading frames(vp1, vp2, vp3) and a regulative region. comparing sequence of the genome through blast in genbank, this sequence exhibits 96.9% identity with other genome of ca vs and least. multiple alignment of this genome of ... | 2002 | 12557549 |
| molecular characteristics of full-length genomic segment a of three infectious bursal disease viruses in china: two attenuated strains and one virulent field strain. | the full-length cdna of genomic segment a of three infectious bursal disease viruses, two attenuated strains (hz2 and jd1) and one virulent field strain (zj2000), was amplified in a single step by reverse transcription-polymerase chain reaction, cloned into pgem-t easy vector, and sequenced. the full length of cloned segment a contains 3259 nucleotides, which includes two partially overlapping open reading fragments (orfs) orf1 and orf2, flanked by 5' and 3' noncoding regions. these strains shar ... | 2001 | 11785891 |
| serological survey of reticuloendotheliosis virus infection in chickens in china in 2005 to 2015. | reticuloendotheliosis virus (rev) causes an immunosuppressive, oncogenic, and runting syndrome in many avian hosts worldwide. the immunosuppression caused by rev can decrease the antibody levels of vaccines and subsequent increase susceptibility to secondary infections. there are no commercial vaccines or effective drugs to control rev infections at present. to investigate the rev infection status in chickens in china, 25,224 sera from 573 chicken flocks, collected between 2005 and 2015, were me ... | 2017 | 29050414 |
| fowl adenovirus species c serotype 4 is attributed to the emergence of hepatitis-hydropericardium syndrome in chickens in china. | since july in 2015, an emerging infectious disease of hepatitis-hydropericardium syndrome (hhs) was prevalent in chicken flocks in china. to confirm the causative agent and investigate the epidemiology of the disease, a total of 38 chicken flocks including 187 samples from jilin, liaoning, heilongjiang, henan, anhui, hubei, jiangxi, xinjiang, shandong and hunan provinces in china were collected and determined by pcr detection, sequencing, phylogenetic analysis and virus isolation. 81 samples (po ... | 2016 | 27615553 |
| characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in escherichia coli isolated from chickens. | extended-spectrum β-lactamase (esbl)-producing escherichia coli have been frequently isolated from food-producing animals and pose a serious threat to human health. this study collected 195 esbl-producing e. coli isolates from 20 chicken farms and 3 live-bird markets located in northeast china (heilongjiang, liaoning, jilin) and jiangsu province from february 2011 to october 2013. esbl genes, including blactx-m, blatem, and blashv, were detected and characterized, and the susceptibilities of the ... | 2015 | 25785885 |
| genotypic distribution and phylogenetic characterization of enterocytozoon bieneusi in diarrheic chickens and pigs in multiple cities, china: potential zoonotic transmission. | this study investigated diarrheic broiler and layer chickens (<50 days; n=14) and pigs of three age groups (preweaned <30 days, weaned ≈30 to 60 days, and growing >60 days; n=64) for e. bieneusi genotypes in northeast china and evaluated the potential roles of chickens and pigs in zoonotic transmission of microsporidiosis. two 45-day-old layer chickens in city jixi, heilongjiang province and one 23-day-old broiler chicken in city songyuan, jilin province were identified to harbor a human-pathoge ... | 2014 | 25255117 |
| isolation and characterization of a herpesvirus from feral pigeons in china. | a herpesvirus was isolated during a diagnostic investigation of severe cases of conjunctivitis in feral pigeons (columba livia f. domestica). isolates of the virus were recovered from throat swabs of the pigeons followed by inoculation of the swab samples in chicken embryo fibroblasts. pigeons inoculated with the isolated virus had similar clinical signs to those observed in naturally infected birds. transmission electron microscopy revealed viral structures with typical herpesvirus morphology. ... | 2015 | 26542366 |