| [prevalence of anti-hev among swine, sheep and chickens]. | to investigate the prevalence of anti-hev among swine, sheep and chickens. | 2004 | 15340498 |
| amantadine-resistance among h5n1 avian influenza viruses isolated in northern china. | we tested the amantadine-resistance among avian influenza a (h5n1) viruses isolated from chicken in hebei province of northern china from 2001 to 2005, and investigated the amantadine use in this area. plague reduction assay in mdck cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. the m2 sequence analysis revealed that four of five resistant isolates contained the point mutations (ser to asn) at position 31 that could confer resistance to amantadine. these results indica ... | 2008 | 17897729 |
| phylogenetic characterization of newcastle disease virus isolated in the mainland of china during 2001-2009. | twenty newcastle disease virus (ndv) isolates from infected chicken flocks during 2001 to 2009 in china were biologically and genetically characterized. all the 20 ndvs were categorized into velogenic (n=17) and lentogenic (n=3) viruses, respectively, according to the mean death time (mdt) of chicken embryos. velogenic viruses carry the motif (112)r-r-q-k-r/f(117) (n=14) or (112)g-r-q-g-r/l(117) (n=3) at the f0 cleavage site, while all the lentogenic virus had a sequence motif of (112)g-r-q-g-r/ ... | 2010 | 19833457 |
| isolation and analysis of two naturally-occurring multi-recombination newcastle disease viruses in china. | two newcastle disease viruses (ndv), designated qg/hebei/07 and xd/shandong/08, were isolated from infected chicken flocks in china in 2007 and 2008, respectively. the results of phylogenetic and recombination analyses on complete ndv genome sequences (excluding terminal segments) show that the qg/hebei/07 isolate had evidence of recombination in the m and f genes, and recombination in the xd/shandong/08 isolate in the f, l genes and the non-coding region between the hn and l genes. these two na ... | 2010 | 20363269 |
| genetic variation of major histocompatibility complex blb2 gene exon 2 in hebei domestic chicken. | genetic variation of mhc blb2 gene exon 2 in hebei domestic chicken was investigated, after pcr and sequencing of a 374bp fragment (containing entire exon 2 (270bp) of blb2 gene) in 76 individuals. the results showed that along this fragment, there were 69 variable sites, of which 18 were novel variations, and 82 estimated haplotypes with the diversity of 0.960. in hebei domestic chicken, the nucleotide diversity (π), the average number of nucleotide differences (k), the average number of nucleo ... | 2010 | 21075409 |
| prokaryotic expression and identification of 3-1e gene of merozoite surface antigen of eimeria acervulina. | eimeria acervulina was isolated from chicken at hebei province, china. the gene of merozoite surface antigen 3-1e was amplified and cloned into pet28a(+) vector and then transformed into escherichia coli bl21 strain. results showed that 3-1e fusion protein band of about 22 kda was identified by sds-page. western blot analysis indicated that the recombinant protein specifically reacted with e. acervulina polyclonal antibody. | 2011 | 21499749 |
| novel reassortant highly pathogenic h5n2 avian influenza viruses in poultry in china. | there has been multiple evidence that domestic poultry may act as a vessel for the generation of novel influenza a viruses. in this study, we have analyzed the evolution and pathogenicity of 4 h5n2 avian influenza viruses isolated from apparently healthy poultry from h5n1 virus endemic areas in china. phylogenetic analysis revealed that two of these viruses, a/duck/eastern china/1111/2011 (dk/ec/1111/11) and a/goose/eastern china/1112/2011 (gs/ec/1112/11) were derived from reassortment events in ... | 2012 | 23049973 |
| nitrous oxide emissions from a maize field during two consecutive growing seasons in the north china plain. | nitrous oxide (n2o) emissions from a maize field in the north china plain (wangdu county, hebei province, china) were investigated using static chambers during two consecutive maize growing seasons in the 2008 and 2009. the n2o pulse emissions occurred with duration of about 10 days after basal and additional fertilizer applications in the both years. the average n20 fluxes from the ck (control plot, without crop, fertilization and irrigation), np (chemical n fertilizer), sn (wheat straw returni ... | 2012 | 22783628 |
| temporal and spatial characteristics of highly pathogenic avian influenza outbreaks in china during 2004 to 2015. | identifying the temporal and spatial characteristics of highly pathogenic avian influenza (hpai) outbreaks is very important for developing effective and appropriate countermeasures against hpai and promoting sustainable development in the poultry industry. this study aimed to analyze four aspects of the temporal and spatial characteristics of hpai outbreaks in china, including the frequency of hpai outbreaks, numbers of dead animals (died or culled), types of hpai viruses, and species of infect ... | 2017 | 28453859 |