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identification of nontuberculous mycobacteria in clinical samples using molecular methods: a 3-year study.nontuberculous mycobacteria (ntm) are being increasingly isolated in clinical laboratories and present technical and therapeutic challenges. in the present study, we report our experience with the identification of ntm received from 12 lisbon hospitals over a 3-year period using genotype mycobacterium (cm/as) assays (hain lifescience gmbh, nehren, germany). together, the two kits identified 96.6% of all ntm isolates tested. among the 18 ntm species identified, mycobacterium avium complex was the ...201019832711
is1245 rflp analysis of strains of mycobacterium avium subspecies hominissuis isolated from pigs with tuberculosis lymphadenitis in portugal.from the end of 2004 increasing numbers of tuberculosis-like lesions were detected in regular meat inspections of pigs at regional abattoirs. mycobacterium avium subspecies hominissuis was identified as the cause of this outbreak of tuberculosis lymphadenitis by is901 and is1245 duplex pcr and fr300 pcr. in order to detect the source and route of infection, is1245 restriction fragment length polymorphism (rflp) analysis was used to type 54 strains (50 from pigs, one from a cow, two from wild boa ...200919168882
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