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identification and quantification of uncultivated proteobacteria associated with pyrene degradation in a bioreactor treating pah-contaminated soil.uncultivated bacteria associated with the degradation of pyrene in a bioreactor treating soil contaminated with polycyclic aromatic hydrocarbons (pah) were identified by dna-based stable-isotope probing (sip) and quantified by real-time quantitative pcr. most of the 16s rrna gene sequences recovered from (13)c-enriched dna fractions clustered phylogenetically within three separate groups of beta- and gamma-proteobacteria unassociated with described genera and were designated "pyrene groups 1, 2 ...200616958754
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