Publications

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evaluation of culicoides insignis (diptera: ceratopogonidae) as a vector of bluetongue virus.based upon epidemiological evidence, culicoides insignis lutz is a probable biological vector of bluetongue viruses (btv) in south florida, the caribbean region and central america. the vector potential of this species for btv was evaluated in the laboratory in a series of experiments using insects caught in the field. although there was great variation in the percentage of flies that fed from any one catch, it was demonstrated that c. insignis became infected after membrane feeding on a mixture ...19921325085
bluetongue in the united states.the virus of bluetongue (bt) was 1st isolated and identified in the us in 1952 from sheep in california. a disease in sheep in texas (soremuzzle) was observed in 1947, reported in 1952 and similarities to bt were discussed. it is possible bt existed in texas for several years prior to that time. there are 23 immunologic serotypes of bt, and 5 are known to occur in the us. these are types 10, 11, 13, 17 and 2, the latter having been recognized only in 1983 in cattle in florida. in the us, bt was ...19852989874
host responses of worm-free florida native and rambouillet lambs experimentally infected with haemonchus contortus. 19725017872
validation of the famacha eye color chart for detecting clinical anemia in sheep and goats on farms in the southern united states.recent studies on sheep and goat farms in the southern united states indicate that multiple-anthelmintic resistance in haemonchus contortus is becoming a severe problem. though many factors are involved in the evolution of resistance, the proportion of the parasite population under drug selection is believed to be the single most important factor influencing how rapidly resistance develops. therefore, where prevention of resistance is an important parallel goal of worm control, it is recommended ...200415265575
rabies surveillance in the united states during 2004.during 2004, 49 states and puerto rico reported 6,836 cases of rabies in nonhuman animals and 8 cases in human beings to the cdc, representing a 4.6% decrease from the 7,170 cases in nonhuman animals and 3 cases in human beings reported in 2003. approximately 92% of the cases were in wildlife, and 8% were in domestic animals (compared with 91% and 9%, respectively, in 2003). relative contributions by the major animal groups were as follows: 2,564 raccoons (37.5%), 1,856 skunks (27.1%), 1,361 bat ...200516379626
accuracy of the famacha system for on-farm use by sheep and goat producers in the southeastern united states.famacha is a practical on-farm system designed to provide small ruminant producers a tool for improving their management of haemonchus contortus infections. although this system has become very popular and widely accepted by small ruminant producers in many regions of the southern united states, there is very limited data reported on the effectiveness of the famacha system when performed by farmers. the objective of this study was to evaluate the accuracy of the famacha system for on-farm use by ...200717482368
growth and development of the ovine conceptus.this study was designed to characterize development of the ovine conceptus throughout gestation to establish the temporal relationships in metabolites, electrolytes, fluid volumes within the placenta, and hormonal changes with fetal growth. length and weight of placentae, weight of cotyledons, and uterine weight increased between d 25 and d 80 of gestation in advance of increases in fetal growth between d 80 and d 140 of gestation. allantoic fluid volumes changed (p < 0.01) between d 25 (21 ml) ...201121841083
introduction of potential heartwater vectors and other exotic ticks into florida on imported reptiles.following the discovery of establishment of the african tortoise tick amblyomma marmoreum in florida, the present study was undertaken to determine the extent of introduction of exotic ticks into florida on imported reptiles. exotic ticks were identified on 29 (91%) of 32 reptile premises in 18 counties of florida. the ticks, found on a variety of imported tortoises, snakes, and monitor lizards, belonged to 4 amblyomma species (a. marmoreum, amblyomma nuttalli, amblyomma sabanerae, and amblyomma ...200010958443
serological observations on the epidemiology of bluetongue virus infections in the caribbean and florida.serological surveys of cattle, sheep and goats have confirmed that infection with bluetongue virus (btv) is common in florida, puerto rico and st. croix in the usa, in the caribbean countries of jamaica, st. kitts/nevis, antigua, st. lucia, barbados, grenada, trinidad and tobago, and the bahamas, and in the south american countries of guyana and suriname. in most countries, over 50% of ruminant livestock have antibody to btv as assessed by the bluetongue immunodiffusion (btid) test. a sentinel a ...19852989905
seasonal transmission of fasciola hepatica in north central florida (u.s.a.).to assess seasonal transmission of fasciola hepatica 37 groups of fluke-free tracer sheep, one group of four sheep each month in succession, grazed an infected pasture in north central florida (u.s.a.) from june 1984 to june 1987. at the end of each month, tracers were moved to a fluke-free barn for 2 months, then necropsied and flukes counted. fluke transmission mostly occurred during the first half of the year, with peak transmission from february to april. no transmission occurred during the ...19902228432
two electropherotypes of bluetongue virus serotype 2 from naturally infected calves.the first isolation of bluetongue virus (btv) serotype 2 in the u.s.a. was in 1982 from a sentinel herd of cattle at ona, florida. electrophoretic analysis of genome rna revealed that all 16 serotype 2 isolates obtained from this focus of infection had one of two electropherotypes (designated ona a and ona b). all genome segments of ona a and ona b had detectable differences in electrophoretic mobility, with major differences noted for segments 1, 4, 5, 7, 8, 9 and 10. electrophoretic comparison ...19852989416
outbreak of shiga toxin-producing escherichia coli o157 infection associated with a day camp petting zoo--pinellas county, florida, may-june 2007.on june 7, 2007, the pinellas county health department in central florida was notified by a private physician regarding a positive laboratory result for shiga toxin-producing escherichia coli o157 (stec o157) infection in a child aged 9 years. initial interviews revealed the child had attended a week-long session at a day camp and had come into contact with animals in the camp's petting zoo. on june 8, an investigation was begun by the pinellas county health department; the same day, the petting ...200919407735
petting zoos. 200616719427
value of desiccated swabs for streptococcal epidemiology in the field.streptococcal surveys in foreign countries or remote areas may be greatly enhanced by the use of calcium alginate swabs desiccated in sterile silica gel. delays of up to 4 weeks before return to a base laboratory are feasible, and the need for fresh media or laboratory facilities in the field may be eliminated. comparison of direct plating on crystal violet blood agar versus delayed silica gel preservation during surveys in uganda, haiti, colombia, and miami, fla., showed no significant loss of ...19734346975
isolation and identification of bluetongue virus: a serotype new to the u.s.there are 5 known serotypes of bluetongue (bt) virus (btv) in the us: 2, 10, 11, 13 and 17. the most recently discovered us serotype, btv 2, was isolated from blood collected from cattle at ona, florida in 1982. isolations were made from the september through november bleedings and from 1 pool of culicoides insignis lutz collected in october. seventeen viral isolates were obtained by injecting the samples into embryonated chicken eggs (ece) followed by serial passage onto bhk-21 cells. a single ...19852989869
persistence of bluetongue virus serotype 2 (btv-2) in the southeast united states.the prototype united states (us) strains of bluetongue virus serotype 2 [btv-2 (onaa) and btv-2 (onab)] made in florida in 1982 were compared to a recent btv-2 (fl99) isolate made in florida in 1999 to determine if the original strain(s) had persisted or if a new strain of btv-2 had been re-introduced into the southeast us. viral rna and protein electropherotypes, and sequence analysis of five rna genome segments for these early and later btv-2 isolates were compared. these comparisons indicated ...200515946757
pseudoepidemic of q fever at an animal research facility.serum samples from people exposed to sheep at a research facility were evaluated by a commercial laboratory and resulted in an overall coxiella burnetii seroprevalence of 75%. we interviewed individuals to determine exposure history and compatible illness, and retested their sera. analysis indicated that the commercial laboratory was misinterpreting its results; when corrected, the seroprevalence dropped to 27%. test kits of the brand used by the commercial laboratory gave equivalent results to ...200415682517
129i and 137cs fission products in thyroids of animals, 1984-1991.iodine is intensely concentrated in the thyroid of animals, while 137cs is not. in this study, 129i and 137cs concentrations were determined in animal thyroids from selected areas during 1984-1991. the thyroids were from deer killed within the savannah river site (srs) in south carolina; from the environs of oak ridge (or), tennessee; west tennessee; and florida. thyroids from sheep slaughtered routinely in birmingham, england (uk), were also tested. the glands were analyzed by x-ray spectroscop ...19938416215
crystallization and toxicology of t34: a major toxin from florida's red tide organism (ptychodiscus brevis). 19817199210
observations on larval habitats of suspected culicoides vectors of bluetongue virus in florida.potential breeding sites for culicoides variipennis (coquillett) and c. insignis lutz, suspected vectors of bluetongue virus (btv) in florida, were examined at 7 livestock facilities located in different geographic regions of the state. the 2 most productive habitats were mud contaminated by effluent from milking parlors (92.3 larvae/sample) and margins of vegetated ponds (18.8 larvae/sample). four species of biting midges, c. crepuscularis malloch, c. haematopotus malloch, c. insignis, and c. v ...19852989856
orbiviruses from culicoides in florida.between 1980 and 1983, 45,484 culicoides spp. collected in florida near cattle have been examined for orbiviruses by attempted isolation in cell cultures and intravenous (iv) inoculations of embryonated chicken eggs. bluetongue virus (btv) serotype 2 was isolated from a pool of culicoides insignis trapped at ona. this is the 1st recovery of btv from this species representing the 2nd new world species of culicoides from which bt viruses have been isolated. c. insignis is a neotropical form which ...19852989853
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