haemonchus contortus resistance to ivermectin and netobimin in brazilian sheep.suffolk, texel, hampshire down and ile de france sheep from the municipalities of porto amazonas, piraquara and araucaria in the state of paraná, and bagé in the state of rio grande do sul were brought to sobral, state of ceará, to be used in a cross-breeding project. on arrival they had clinical signs of nematode parasitosis, and one suffolk female died. the animals were treated orally with ivermectin (0.2 mg kg-1) and fifteen days later with netobimin (20.0 mg kg-1). neither drug reduced the e ...19921485411
occurrence of resistance to anthelmintics in sheep in paraná state, brazil. 19968923717
prevalence of neospora caninum and toxoplasma gondii in sheep and dogs from guarapuava farms, paraná state, brazil.sheep and dog blood samples were collected from nine farms in the county of guarapuava, paraná, brazil. the indirect fluorescent antibody test (ifat) was used to detect neospora caninum and toxoplasma gondii antibodies. herein, serum samples from 305 sheep were evaluated, being 29 (9.5%) and 157 (51.5%) seropositives to n. caninum and t. gondii, respectively. seven (29.1%) and five (20.8%) out of 24 dogs were seropositives to n. caninum and t. gondii, respectively. there were no differences amon ...200717266999
[occurrence of anti-toxoplasma gondii antibodies in swine and ovine slaughtered at municipality of guarapuava in the state of paraná, brazil].this study evaluated sero-occurrence of toxoplasmosis in swine and ovine slaughtered in the municipality of guarapuava in the state of paraná. blood samples from 117 swine and 157 ovine were collected. indirect immunofluorescence assay (ifa) was used to detect igg anti-toxoplasma gondii antibodies. serum samples were considered positive at dilutions >or=1:64. out of 117 swine blood sera, 10 (8.54%) were positive, while 11 out of 157 ovine samples (7.0%) showed a reaction to t. gondii antibodies. ...200717588324
prion protein gene polymorphisms in sheep in the state of paraná, determine the polymorphisms of the prion protein gene in sheep from the state of paraná, brazil, 323 animals of meat breeds (suffolk, hampshire down, texel, ile de france, dorper, dorset, santa inês and crossbreds) were genotyped by restriction fragment length polymorphism (rflp) analysis. the most frequent allele was arq, with a frequency of 0.61, followed by arr (0.30). vrq and ahq alleles were present at very low frequencies (0.13 and 0.05 respectively), and the arh allele was not found. s ...200818752582
[occurrence of anti-toxoplasma gondii antibodies in ovine from urban and periurban areas from curitiba, parana state].this study evaluated occurrence of the toxoplasma gondii infection in ovine from urban and periurban areas of curitiba, state of paraná, southern of brazil. blood samples from 167 sheep from three farms were collected. elisa assay was used to detect igg anti-toxoplasma gondii antibodies. of 167 ovine samples, 43 (25.75%) were positive. the parasite is widespread among sheep from urban and periurban areas of curitiba.200920040195
triclabendazole resistance involving fasciola hepatica in sheep and goats during an outbreak in almirante tamandare, paraná, brazil.fasciolosis is a disease of extreme importance, occurring throughout brazil, with great economic losses to the animal industry. this study aims to determine the effectiveness of treatment against fasciola hepatica on a sheep and goat farm during an outbreak of fasciolosis, in which a high proportion of deaths occurred. the farm harbored 33 sheep and 60 goats of mixed breeds with varying weights and ages. parasite control was based on suppressive, monthly treatments with moxidectin. over the cour ...200820059835
preliminary evaluation of hitchens-pike-todd-hewitt medium (hpth) for detection of group b streptococci in pregnant women.we aimed to compare hitchens-pike-todd-hewitt (hpth) enrichment broth, todd-hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (aba) in screening group b streptococci (gbs) carriage in pregnant women. from april to june 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at santa casa de misericórdia hospital, maringá, paraná, brazil, were screened by gbs using hpth, todd- ...201021089171
virulence determinants in vancomycin-resistant enterococcus faecium vana isolated from different sources at university hospital of londrina, paraná, brazil.enterococcus faecium, especially those showing multidrug resistance, has emerged as a significant cause of healthcare-associated infections worldwide. however, relatively little is known about the virulence and pathogenesis of this species. the aim of this study was to determine the occurrence of four putative virulence determinants of e. faecium and to correlate them with phenotypic traits. using forty e. faecium vana-type isolates from hospitalized patients and their environmental vicinity, we ...201021221940
Serological survey of paracoccidioidomycosis in sheep.The objective of this study was to evaluate the prevalence of antibodies against Paracoccidioides brasiliensis in sheep from Guarapuava, Paraná State, Brazil. The seroepidemiological study was carried out in 262 sheep. The samples were analyzed by ELISA and immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively. Initially, two sheep were immunized with P. brasiliensis to evaluate whether contact with the fungal cells could induce a humoral immune response agains ...201221850559
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