faecal shedding and strain diversity of listeria monocytogenes in healthy ruminants and swine in northern spain.listeria monocytogenes is among the most important foodborne bacterial pathogens due to the high mortality rate and severity of the infection. l. monocytogenes is a ubiquitous organism occasionally present in the intestinal tract of various animal species and faecal shedding by asymptomatically infected livestock poses a risk for contamination of farm environments and raw food at the pre-harvest stages. the aim of this study was to determine the prevalence and strain diversity of l. monocytogene ...200919133125
flock-prevalence of border disease virus infection in basque dairy-sheep estimated by bulk-tank milk analysis.bulk-tank milk (btm) samples from 154 sheep flocks were used to estimate bdv prevalence in the basque country in spain using an elisa and a rt-pcr test. the proportion of antibody-positive flocks was 68% but varied significantly between provinces and was 93% in araba and 54-55% in bizkaia and gipuzkoa. most elisa-positive flocks had very low antibody inhibition percentage (aip) indicating high seroprevalence and recent bdv exposure. however, only 9% flocks were pcr-positive suggesting few infect ...200616979308
genetic typing and prevalence of border disease virus (bdv) in small ruminant flocks in spain.between 2001 and 2002, samples from 1,413 animals in 21 spanish small ruminant flocks, most of them with animals showing clinical signs compatible with border disease (bd), were screened for the presence of pestivirus antigen and antibodies by an indirect peroxidase monolayer assay (ipma) and the virus neutralization test (vnt), respectively. although all flocks harboured seropositive animals, virus could only be isolated from animals in five of the flocks. between 4 and 11 months later all anim ...200616857326
tick-borne zoonotic bacteria in wild and domestic small mammals in northern spain.the prevalence and diversity of tick-borne zoonotic bacteria (borrelia spp., anaplasma phagocytophilum, coxiella burnetii, and spotted fever group rickettsiae) infecting 253 small mammals captured in the basque country (spain) were assessed using pcr and reverse line blot hybridization. trapping sites were selected around sheep farms (study 1, 2000 to 2002) and recreational parks (study 2, 2003 to 2005). the majority of the studied mammals (162) were wood mice (apodemus sylvaticus), but six othe ...200717693556
short communication: investigation of coxiella burnetii occurrence in dairy sheep flocks by bulk-tank milk analysis and antibody level estimate the prevalence of coxiella burnetii in the dairy sheep population from the basque country (northern spain), a study was carried out combining molecular and serological techniques. first, bulk-tank milk samples from 154 flocks belonging to the latxa breed farmers association were analyzed by pcr, with 22% of flocks testing positive for c. burnetii. then, a selection of 34 flocks (7 pcr positive and 17 negative) was investigated for the presence of serum antibodies by elisa test on 1,0 ...200919307639
dog echinococcosis in northern spain: comparison of coproantigen and serum antibody assays with coprological exam.a large sheep-dog population from the province of alava (northern spain) has been investigated in order to determine the prevalence of the cestode parasite echinococcus granulosus. worms were detected in 14.0% of 721 dog faecal supernatants by coproantigen elisa, and in 9.1% of 754 dog serum samples by serum antibody elisa. a weak but statistically significant correlation (spearman's rho=0.103, 95% ci: 0.023-0.178) between the two immunoassay results was found. in addition, eggs of the family ta ...200616863681
prevalence and seasonal activity of ixodes ricinus (acari:ixodidae) on domestic ruminants of the basque country, spain.ixodes ricinus on domestic ruminants was sampled through a natural year at several points in the basque country, north spain. the sampling points were comprised of the different natural regions, according to climate and vegetation, into which the region is commonly divided. both the immature and adult stages were collected on cattle and sheep at five of the eight collecting points, namely in areas with a medium elevation, mild summers and a medium to high annual rainfall. the adults of this tick ...19979057304
identification, genetic diversity and prevalence of theileria and babesia species in a sheep population from northern spain.the genetic diversity and prevalence of virtually all theileria and babesia species in a sheep population were studied using a specifically designed reverse line blot macroarray. the amplified hypervariable v4 region of the 18s rrna gene was hybridised against generic and species-specific probes. in a first screening (study i), 320 apparently healthy animals corresponding to 32 flocks located in the basque country (northern spain) were analysed. the survey demonstrated a high prevalence of subcl ...200415313132
genetic parameters and trends for milk production of blond-faced latxa sheep using bayesian analysis.the genetic progress attained with the breeding scheme of the blond-faced latxa dairy sheep in the spanish basque country was assessed by a bayesian approach based on the marginal posterior distributions of parameters achieved via gibbs sampling. the data file included 49,056 milk yield records of 22,363 ewes. normal distributions were assumed for ebv and fixed effects, and scaled inverted chi-square distributions were assumed for variance components or additive, permanent environment, and resid ...19969029366
two ovine mitochondrial dnas harboring a fifth 75/76 bp repeat motif without altered gene expression in northern spain.the basque country is home to the latxa sheep breed, which is divided in several varieties such as latxa black face (lbkf) and latxa blonde face (lblf). mitochondrial dna control region analysis of 174 male sheep (97 lbkf and 77 lblf) was performed with the objective of characterizing the maternal lineages of these two varieties that are the basis to produce the cheese with idiazabal quality label. the percentage of unique haplotypes was 77.32% in lbkf and 67.53% in lblf. most of the individuals ...201727990652
first record of culicoides imicola, the main vector of bluetongue virus in europe, in the basque country (northern spain). 200818567930
prp polymorphisms in basque sheep breeds determined by pcr-restriction fragment length polymorphism and real-time pcr.two new pcr-based methods were developed to decode prion protein (prp) gene polymorphisms at codons 136, 154 and 171: a pcr-restriction fragment length polymorphism (rflp) analysis consisting of two pcr reactions followed by three enzymatic digestions, and a real-time pcr consisting of four reactions with seven fluorogenic probes. both methods were used to study the distribution of prp gene polymorphisms in a representative sample (1297 animals) of the populations of the two native breeds of she ...200415214515
atypical/nor98 scrapie in the basque country: a case report of eight outbreaks.since 2002, an active surveillance program for transmissible spongiform encephalopathy in small ruminants in european union countries allowed identification of a considerable number of atypical cases with similarities to the previously identified atypical scrapie cases termed nor98.201020346155
q fever: prevalence of antibodies to coxiella burnetii in the basque country.the aim of this study was to ascertain the prevalence of coxiella burnetii infection in the basque population. to this end a stratified sampling was carried out taking as a criterion the size of the population in a given area of residence. residents in sparsely populated areas were found to have a prevalence of 38.5%, significantly greater than in highly populated areas (odds ratio [or] = 1.56; 95% confidence interval [ci]: 1.08-2.27 and or = 1.58; 95% ci: 1.09-2.30). the prevalence was signific ...19938144303
comparison of models for describing the lactation curve of latxa sheep and an analysis of factors affecting milk yield.the objectives of this work were 1) to compare the goodness-of-fit of empirical models of the lactation curve and 2) to analyze the factors affecting the shapes of the lactation curves, the parameters describing them, and the overall milk yield of latxa dairy sheep. a total of 14,699 records from 2711 ewes, collected during three consecutive years (1995 to 1997) by the milk recording program of the latxa ewe of the basque country (spain), were used. six mathematical models and three fitting proc ...200011104292
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