[an epidemiologic analysis of brucellosis in liaoning province].in order to understand the increasing incidence rate of brucellosis, the relationship between the epidemic trend and physical conditions of humans, data regarding brucellosis in liaoning province, in 1990-1996 was analysed. results showed that the major cause which led to the brucellosis recurrence was the importation of sheep named short-tail cold-resistant. the distribution of disease was widely spread. however, most endemic foci were being expanded. the spots in tieling and fushun area were m ...199810322712
at least two genetically distinct large babesia species infective to sheep and goats in china.a fatal disease of sheep and goats in the northern part of china has been reported to be due to babesia ovis. however, some characteristics of the causative agent in recent reports are not in accordance with the original attributes ascribed to this parasite. therefore, the 18s small subunit ribosomal rna (18s rrna) genes of a number of babesia isolates in china were sequenced and compared with that of other babesia and theileria species in an attempt to clarify their taxonomic position. in the p ...200717531391
seroepidemiology and genetic characterization of hepatitis e virus in the northeast of china.the aim of this study was to analyze the prevalence of infection and genotype of hepatitis e virus (hev) in people and animals in the northeast of china (heilongjiang, jilin and liaoning provinces). this seroepidemiological study was conducted using enzyme immunoassays and human sera positive for hev antigen or anti-hev igm, and animal sera positive for hev antigen or with an s/co <or=10 for anti-hev were tested for hev rna using real-time rt-pcr and nested rt-pcr. in humans, the overall prevale ...200919460321
mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau--focused on the qinghai area, investigation was conducted to identify the distribution of mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau, china from july to august in 2007. a total of 8,147 mosquitoes representing six species from three genera (aedes, culex, and anopheles) were collected in three locations (geermu city, altitude of 2,780 m; xining city, 2,200 m; minhe county, 1,700 m). six virus isolates were obtained including tahyna virus (tahv), liaoning virus, and culex pipiens pallens densovir ...201020348523
characterization of the ghr gene genetic variation in chinese indigenous goat breeds.the aim of the present work was to investigate single nucleotide polymorphism (snp) of growth hormone receptor (ghr) gene exon 10, characterize the genetic variation in three chinese indigenous goat breeds, and search for its potential association with cashmere traits. in this study, a polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) protocol has been developed for rapid genotyping of the ghr gene in goats. one hundred seventy-eight goats from liaoning cashmere (96), ...201120364329
mlva typing and antibiotic susceptibility of brucella human isolates from liaoning, china.we have characterized clinical isolates of brucella melitensis from liaoning, china, using multiple-locus variable-number repeat analysis of 16 loci (mlva-16) and evaluated the antimicrobial susceptibilities in vitro. all 31 isolates shared the panel 1 genotype 42 profile suggesting that they belong to the 'east mediterranean' group. in vitro antibiotic susceptibility was determined by the broth microdilution testing. all isolates were sensitive to tested antibiotics (rifampin, doxycycline, cipr ...201020832094
polymorphisms of caprine gdf9 gene and their association with litter size in jining grey goats.the exons 1, 2 and flanking region of growth differentiation factor 9 (gdf9) gene in five randomly selected does of jining grey, boer and liaoning cashmere goats were amplified and analyzed. thirteen nucleotide differences were identified in gdf9 gene between sheep (af078545) and goats. four snps (g3288a in intron 1, g423a, a959c [gln320pro] and g1189a [val397ile] in exon 2) were detected in four goat breeds with different prolificacy, in which g3288a was a new snp in goats. the results showed t ...201021181498
seroprevalence of toxoplasma gondii infection in domestic sheep in liaoning province, northeastern china.there is a lack of information regarding the prevalence of toxoplasma gondii infection in sheep from northeastern china. in the present study, serum samples from 566 domestic sheep were collected from liaoning province, northeastern china, to investigate the prevalence of t. gondii antibodies using an indirect hemagglutination antibody test. twenty-five of 566 samples (4.4%) were seropositive at the cutoff of 1:64 serum dilution. no difference was found among 6 geographic regions (p > 0.05). thi ...201322765359
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