laboratory and field studies of an antigen capture elisa for bluetongue improved bluetongue antigen capture elisa (btace) technique was evaluated for its ability to detect the full range of 24 bluetongue (blu) serotypes. the btace detected all 24 serotypes in cell culture fluids, including eight serotypes where the representative strains originated from both australia and also from the south african reference collection. the amount of infectious virus required to obtain a positive btace result varied between 100-1000 tcid50. this was approximately 10-fold more se ...200010716347
identification of seven serotypes of bluetongue virus from the people's republic of serotypes (1, 2, 3, 4, 12, 15 and 16) of bluetongue virus were isolated from the blood of sheep and cattle in the people's republic of china between 1986 and 1996. six of these viruses were isolated in yunnan province. the sheep from which serotypes 1 and 16 were isolated showed obvious signs of bluetongue disease, whereas the cattle from which serotypes 2, 3, 4, 12 and 15 were isolated were clinically normal. phylogenetic analyses of these viruses indicate that they are more closely relat ...199910755588
studies on the epidemiology of bluetongue virus in china.sentinel herds of large ruminants were established at five centres in yunnan province, peoples republic of china, between 1995 and 1997. the application of a sensitive antigen capture elisa to facilitate virus isolation procedures led to the isolation of 108 strains of bluetongue (blu) virus. serotypes isolated included types 1, 2, 3, 4, 9, 11, 12, 15, 16, 21 and 23. virus transmission occurred over a period of 1-3 months at each of the four positive sites, giving an overall blu virus transmissi ...200212002544
[melanin traits of yunnan black bone sheep and tyr gene polymorphism].the "black bone and muscle" is cardinal melanin trait of black bone sheep. the black bone sheep and the native sheep in lanping and luomuni sheep were chosen as materials for the measurement of tyr activity of blood and melanin content of tissues and organs. moreover, we compared characteristic structure of melanin of black bone sheep with silky fowls. the results showed as follows: tyr activity was significantly different between black and non-black bone sheep (p<0.05); the total character of i ...200616551595
genetic differentiation of native sheep populations in east and south asia.variations of four sheep populations in china were examined by multiloci electrophoresis, and similar data are quoted to analyze the degree of genetic differentiation of native sheep populations in east and south asia. among 15 populations, the average heterozygosity is 0.2746, and the effective number of alleles is 1.559. mongolian sheep possess the highest genetic diversity, and diversity decreases sequentially in the chinese, vietnamese, bangladeshi, and nepalese populations. coefficients of ...200717318371
genomic reconstruction of the history of native sheep reveals the peopling patterns of nomads and the expansion of early pastoralism in east asia.china has a rich resource of native sheep (ovis aries) breeds associated with historical movements of several nomadic societies. however, the history of sheep and the associated nomadic societies in ancient china remains poorly understood. here, we studied the genomic diversity of chinese sheep using genome-wide snps, mitochondrial and y-chromosomal variations in > 1,000 modern samples. population genomic analyses combined with archeological records and historical ethnic demographics data reveal ...201728645168
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