| identification and differentiation of fasciola hepatica and fasciola gigantica using a simple pcr-restriction enzyme method. | accurate morphological differentiation between the liver fluke species fasciola hepatica and fasciola gigantica is difficult. we evaluated pcr-restriction enzyme profiles of internal transcribed spacer 1 (its1) that could aid in their identification. fifty f. hepatica and 30 f. gigantica specimens were collected from different hosts in three provinces of iran. for dna extraction, we crushed fragments of the worms between two glass slides as a new method to break down the cells. dna from the crus ... | 2010 | 19769969 |