| importance of primer selection in the application of pcr technology to the diagnosis of bovine leukemia virus. | the polymerase chain reaction (pcr) was used to detect bovine leukemia virus in bovine blood samples. when applied to leucocytes extracted from the blood samples, the standard method of dna extraction gave good correlation with agar gel immunodiffusion, but a method in which 5 microliters of blood was the starting material was unreliable. selection of the primers was important, and differences in results were observed when the pcr method was applied to blood samples from different geographic are ... | 1994 | 7948198 |