Publications

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prevalence of newly isolated, cytopathic small round virus (aichi strain) in japan.cytopathic small round virus (aichi strain), isolated from a patient with oyster-associated gastroenteritis, showed no reaction in the polymerase chain reaction method for enteroviruses or in the enzyme-linked immunosorbent assay (elisa) for the five serotypes of astroviruses. our elisa was sensitive in detecting the aichi strain antigen in stool samples, but there was no reaction in this elisa with any non-aichi strains of enteric viruses, with such origins as enterovirus, rotavirus, norwalk vi ...19938263178
detection of human enteric viruses in japanese clams.a total of 57 clam packages that were collected from supermarkets and fish markets from 11 different sites in western japan between 8 december 2005 and 6 september 2006 were examined for human enteric viruses (i.e., norovirus, aichi virus, rotavirus, adenovirus, hepatitis a virus, and astrovirus), using pcr and reverse transcription pcr. sixty-one percent of the packages were contaminated with one type of virus, 9% had two different types of viruses, 28% had three different types of viruses, and ...200818724766
a novel rt-multiplex pcr for detection of aichi virus, human parechovirus, enteroviruses, and human bocavirus among infants and children with acute gastroenteritis.a novel reverse transcription-multiplex polymerase chain reaction assay was developed to detect aichi virus, human parechovirus, enteroviruses, and human bocavirus. a mixture of four pairs of published specific primers, 6261 and 6779, ev22(+) and ev22(-), f1 and r1, 188f and 542r, was used to amplify the viral genomes and specifically generate four different amplicon sizes of 519, 270, 440, and 354 bp for aichi virus, human parechovirus, enteroviruses, and human bocavirus, respectively. a total ...201020691209
a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea.a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ...201121349292
isolation and molecular characterization of aichi viruses from fecal specimens collected in japan, bangladesh, thailand, and vietnam.aichi virus is a new member of the family picornaviridae, genus kobuvirus, and is associated with human gastroenteritis. this study detected aichi virus in 28 of 912 fecal specimens which were negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus and were collected in japan, bangladesh, thailand, and vietnam during 2002 to 2005.200717522267
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