| epidemiological and virological studies on outbreaks of acute gastroenteritis associated with rotavirus in primary schools in osaka. | there have been three recent outbreaks of acute gastroenteritis in primary schools in osaka prefecture caused by rotavirus: one in a school (ta) in april, 1974 and two in two other schools (te and k) in may 1975. the morbidity from the disease was 2.4--15.8% for all age groups in the schools, and 20.1--34.1% for a certain age group. the disease lasted for 4 (k) to 14 days (te). the first cases in schools ta and te were followed by successive cases. the main clinical symptoms were higher frequenc ... | 1979 | 231434 |
| emergence of new variant rotavirus g3 among infants and children with acute gastroenteritis in japan during 2003-2004. | a total of 402 fecal specimens from infants and children with acute gastroenteritis in five places (tokyo, maizuru, saga, sapporo, and osaka) in japan from july 2003 to june 2004 were collected and then tested for the presence of rotavirus by rt-pcr. of these, 83 were positive for rotavirus and this accounted for 20.6%. rotavirus was further characterized to g-types (vp7 genotypes) and p-types (vp4 genotypes). interestingly, an emergence of rotavirus g3 was identified with an exceptionally high ... | 2007 | 17323824 |
| molecular and epidemiological trend of rotavirus infection among infants and children in japan. | this study investigated the prevalence of group a, b, and c rotavirus (rav, rbv, rcv) and adenovirus (adv) infections in infants and children with acute gastroenteritis in japan from july 2006 to june 2007. a total of 628 fecal specimens collected from infants and children with acute gastroenteritis in five different places (maizuru, tokyo, sapporo, saga and osaka) in japan during the period of july 2006-june 2007 were examined for rav, rbv, rcv and adv by rt-multiplex pcr. rav was further chara ... | 2009 | 19560560 |
| detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in japan, 2007-2009. | the molecular epidemiology of rotavirus infections in non-hospitalized children in five different regions (sapporo, saga, tokyo, osaka, and maizuru) of japan during 2007-2009 was investigated. overall, rotavirus was detected in 156 out of 1008 (15.5%) specimens. the rotavirus infection in 2007-2008 (19.3%) was higher than those in 2008-2009 (12.1%). g1p[8] was the most prevalent (62.8%), followed by g3p[8] (21.8%), g9p[8] (14.7%), and g2p[4] (0.7%). interestingly, the number of g3p[8] strains in ... | 2010 | 21145986 |
| detection of human parechovirus in stool samples collected from children with acute gastroenteritis in japan during 2007-2008. | of 477 stool specimens, which had been screened for rotavirus, adenovirus, norovirus, sapovirus and astrovirus, collected from infants and children with acute gastroenteritis in pediatric clinics encompassing five localities (sapporo, tokyo, maizuru, osaka, and saga) in japan from july 2007 to june 2008, 247 negative samples (51.7%) were subjected to screening for human parechovirus. human parechovirus (hpev) was detected by rt-pcr using a primer pair to amplify 5'utr region of its genome and wa ... | 2011 | 21181931 |
| genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan in 2004-2005. | a total of 752 fecal specimens collected from july 2004 to june 2005 from children with acute gastroenteritis in four localities in japan (maizuru, tokyo, sapporo, and osaka) were screened for group a rotavirus by rt-pcr. it was found that 82 (10.9%) specimens were positive for group a rotavirus. the g-(vp7 genotypes) and p-(vp4 genotypes) types were further investigated. the p-types of 18 rotavirus strains, which could not be typed by rt-pcr, were determined by sequencing analysis. of these, 94 ... | 2007 | 17110174 |
| outbreak of sapovirus infection among infants and children with acute gastroenteritis in osaka city, japan during 2004-2005. | one hundred and twenty five fecal specimens were collected from sporadic cases of acute gastroenteritis in a pediatric clinic in osaka city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (19.2%, 24 of 125), followed by group a rotavirus (18.4%, 23 of 125), astrovirus (1.6%, 2 of 125), and adenovirus (0.8%, 1 of 125), respectively. ... | 2006 | 16628578 |
| three-year survey of the epidemiology of rotavirus, enteric adenovirus, and some small spherical viruses including "osaka-agent" associated with infantile diarrhea. | studies were made by electron microscopy (em) on the viruses associated with diarrhea of outpatients at a pediatric clinic in osaka prefecture during the three year period from 1980 through 1982. the viruses detected by em by negative staining with phosphotungstic acid (pta) were classified morphologically into 6 groups: rotavirus, adenovirus and four kinds of small spherical viruses, calicivirus, astrovirus, picornavirus/parvovirus (p/p)-like agent and osaka-agent. osaka-agent seems to be a new ... | 1985 | 3000350 |
| viral diarrhea in japanese children: results from a one-year epidemiologic study. | a total of 557 fecal specimens from infants and children with acute gastroenteritis in five places (maizuru, tokyo, sapporo, saga and osaka) in japan from july 2002 to june 2003 were tested for the presence of diarrheal viruses by rt-pcr, prha, rna-page and latex agglutination methods. of these, 56.4% (314) were found positive for diarrheal viruses. among them, group a rotavirus was the most prevalent (43.6%, 137 of 314) followed by norovirus (29.9%, 94 of 314), adenovirus (7.6%, 24 of 314), gro ... | 2005 | 15819174 |
| etiologic agents of acute gastroenteritis among japanese infants and children: virus diversity and genetic analysis of sapovirus. | a total of 371 fecal specimens from infants and children with acute gastroenteritis in maizuru, tokyo and osaka, japan from july 2002 to june 2003 were tested for the presence of diarrheal viruses by reverse transcription-polymerase chain reaction (rt-pcr), reverse passive hemagglutination (prha), rna-polyacrylamide gel electrophoresis (page), latex agglutination and sequence analysis methods. among diarrheal viruses detected, group a rotavirus was the most prevalent (42.2%) followed by noroviru ... | 2005 | 15789264 |
| serotypes of human rotaviruses in 7 regions of japan from 1984 to 1997. | human rotavirus (hrv) serotypes were studied from diarrheal stool specimens in children in 7 regions of japan (sapporo, tokyo, maizuru, osaka, kagawa, kurume, and saga) from 1984 to 1997 by enzyme immunoassay (eia) with serotype-specific monoclonal antibodies against serotypes 1, 2, 3, and 4. in addition, reverse transcription-polymerase chain reaction (rt-pcr) was conducted for analysis of "others" which included nonserotypable and mixed-serotype rotavirus specimens by eia. in 3756 rotavirus-po ... | 1999 | 10077900 |