| dna and protein sequence conservation at the replication terminus in bacillus subtilis 168 and w23. | cloned dna from the replication terminus region of bacillus subtilis 168 was used to identify and construct a restriction map of the homologous region in b. subtilis w23. with this information, dna from the terminus region of w23 was cloned and the sequence was determined for a 1,499-base-pair segment spanning the expected terc site. the position of the site was then located more precisely. use of the cloned dna from strain w23 as a probe for digests of dna from exponentially growing cells of th ... | 1989 | 2493444 |
| expression of the rtp gene of bacillus subtilis is required for replication fork arrest at the chromosome terminus. | it was earlier proposed that clockwise replication fork arrest at the chromosome terminus in bacillus subtilis is dependent upon expression of the rtp gene adjacent to the site of arrest, terc [smith and wake, j. bacteriol. 170 (1988) 4083-4090]. a merodiploid strain of b. subtilis, in which rtp was placed under the control of the iptg-inducible spac-1 promoter, was constructed. replication fork arrest at terc, as monitored by the level of a forked dna molecule of predicted dimensions, was shown ... | 1989 | 2515996 |
| relocation of the replication terminus, terc, of bacillus subtilis to a new chromosomal site. | the terc-deletion strain of bacillus subtilis 168, su153 [iismaa and wake, j. mol. biol. 195 (1987) 299-310] was used for the re-insertion of a 1.75-kb segment of dna containing terc at a site approx. 25 kb from its original position. the relocated terc in the new strain, su160, was oriented normally with respect to the approaching clockwise replication fork, and was positioned such that this fork was the first to reach it. the relocated terc was effective in causing arrest of the clockwise fork ... | 1988 | 3139495 |
| impediment to replication fork movement in the terminus region of the bacillus subtilis chromosome. | the terminus regions of the chromosomes of three strains of bacillus subtilis 168 were radioactively labelled by supplying [3h]thymine towards the end of a round of replication. these strains lacked or contained the prophage sp beta c2. following restriction endonuclease digestion of the purified dna and fluorography, an sp beta c2-related perturbation of the terminus-labelling profile was observed, which was completely consistent with the previously suggested existence of an impediment to repli ... | 1984 | 6094834 |
| physical map of the bacillus subtilis replication terminus region: its confirmation, extension and genetic orientation. | the library of bacillus subtilis dna previously cloned in the cosmid vector phc79 has been screened for the replication terminus region using a higher level of terminus probe. 24 of 48 recombinant cosmids which gave a positive response had restriction fragment compositions consistent with their inserts originating from or extending into the terminus region for which a 150-kb restriction map has already been constructed (weiss and wake, 1983). dna spanning terc, the site of termination, appears t ... | 1984 | 6442251 |
| replication through the terminus region of the bacillus subtilis chromosome is not essential for the formation of a division septum that partitions the dna. | germinated and outgrowing spores of a temperature-sensitive dna initiation mutant of bacillus subtilis were allowed to initiate a single round of replication by being shifted from 34 to 47 degrees c at the appropriate time. the dna replication inhibitor 6-(parahydroxyphenylazo)-uracil was added to separate portions of the culture at various times during the round. samples were collected from each around the time of the first division septation for measurements of the extent of the round complete ... | 1995 | 7559364 |
| autoregulation of the gene encoding the replication terminator protein of bacillus subtilis. | one of two putative sigma a promoters identified previously in the region immediately upstream from the rtp gene (encoding the replication terminator protein) [smith and wake, j. bacteriol. 170 (1988) 4083-4090] has been shown by transcription start point (tsp) mapping to be the functional rtp promoter. in these tsp mapping experiments, it was observed that the level of mrna from this promoter, prtp, was increased by a factor of 30 in the absence of the replication terminator protein (rtp), cons ... | 1993 | 8406044 |